Phylogenetic and pathotypic characterization of newcastle disease virus in Tibetan chickens, China

Phylogenetic and pathotypic characterization of newcastle disease virus in Tibetan chickens, China

ABSTRACT: Chickens are considered to be potential reservoirs of Newcastle disease virus (NDV). In this study, six Newcastle disease virus strains were isolated and characterized in Tibetan chickens. The HN gene was sequenced, and phylogenetic relationship to reference strains was studied. The phylogenetic analysis demonstrated that these six isolated strains were closely related to NDV isolates of the reference strains GQ245823, KT002186, KU527561, KJ563939, AY225110, EU305607, KM056357, Y18898, GQ245832, AF077761 and lasota strain. Among them, EU305607, KJ563939 and KM056357 were isolated from India, while lasota strain came from attenuated vaccine widely used in China. Then, mean death time (MDT) and intracerebral pathogenicity index (ICPI) were used to estimate the pathogenicity of the isolates. Pathogenicity experiment showed HNH1 and HN17 to be virulent. Our results indicated that genetically diverse viruses circulate in Tibetan chickens, and based upon the phlogeographic analysis, we estimated the origin of ancestral viruses of the isolates and its sister strains located in India and China (lasota strain). It indicates the importance of continuous surveillance to enhance current understanding of the genetic evolution of the NDV strains.

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Main Authors: Zhu,Hongyun, Zhang,Hui, Wang,Yajing, Ciren,Danba, Dong,Hailong, Wu,Qingxia, Rehman,Mujeeb Ur, Nabi,Fazul, Mehmood,Khalid, Li,Jiakui
Format: Digital revista
Language:English
Published: Colégio Brasileiro de Patologia Animal - CBPA 2018
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000100037
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spelling oai:scielo:S0100-736X20180001000372018-04-09Phylogenetic and pathotypic characterization of newcastle disease virus in Tibetan chickens, ChinaZhu,HongyunZhang,HuiWang,YajingCiren,DanbaDong,HailongWu,QingxiaRehman,Mujeeb UrNabi,FazulMehmood,KhalidLi,Jiakui Newcastle disease virus (NDV) Tibetan chickens phylogenetic analysis pathogenicity ABSTRACT: Chickens are considered to be potential reservoirs of Newcastle disease virus (NDV). In this study, six Newcastle disease virus strains were isolated and characterized in Tibetan chickens. The HN gene was sequenced, and phylogenetic relationship to reference strains was studied. The phylogenetic analysis demonstrated that these six isolated strains were closely related to NDV isolates of the reference strains GQ245823, KT002186, KU527561, KJ563939, AY225110, EU305607, KM056357, Y18898, GQ245832, AF077761 and lasota strain. Among them, EU305607, KJ563939 and KM056357 were isolated from India, while lasota strain came from attenuated vaccine widely used in China. Then, mean death time (MDT) and intracerebral pathogenicity index (ICPI) were used to estimate the pathogenicity of the isolates. Pathogenicity experiment showed HNH1 and HN17 to be virulent. Our results indicated that genetically diverse viruses circulate in Tibetan chickens, and based upon the phlogeographic analysis, we estimated the origin of ancestral viruses of the isolates and its sister strains located in India and China (lasota strain). It indicates the importance of continuous surveillance to enhance current understanding of the genetic evolution of the NDV strains.info:eu-repo/semantics/openAccessColégio Brasileiro de Patologia Animal - CBPAPesquisa Veterinária Brasileira v.38 n.1 20182018-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000100037en10.1590/1678-5150-pvb-5039
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Zhu,Hongyun
Zhang,Hui
Wang,Yajing
Ciren,Danba
Dong,Hailong
Wu,Qingxia
Rehman,Mujeeb Ur
Nabi,Fazul
Mehmood,Khalid
Li,Jiakui
spellingShingle Zhu,Hongyun
Zhang,Hui
Wang,Yajing
Ciren,Danba
Dong,Hailong
Wu,Qingxia
Rehman,Mujeeb Ur
Nabi,Fazul
Mehmood,Khalid
Li,Jiakui
Phylogenetic and pathotypic characterization of newcastle disease virus in Tibetan chickens, China
author_facet Zhu,Hongyun
Zhang,Hui
Wang,Yajing
Ciren,Danba
Dong,Hailong
Wu,Qingxia
Rehman,Mujeeb Ur
Nabi,Fazul
Mehmood,Khalid
Li,Jiakui
author_sort Zhu,Hongyun
title Phylogenetic and pathotypic characterization of newcastle disease virus in Tibetan chickens, China
title_short Phylogenetic and pathotypic characterization of newcastle disease virus in Tibetan chickens, China
title_full Phylogenetic and pathotypic characterization of newcastle disease virus in Tibetan chickens, China
title_fullStr Phylogenetic and pathotypic characterization of newcastle disease virus in Tibetan chickens, China
title_full_unstemmed Phylogenetic and pathotypic characterization of newcastle disease virus in Tibetan chickens, China
title_sort phylogenetic and pathotypic characterization of newcastle disease virus in tibetan chickens, china
description ABSTRACT: Chickens are considered to be potential reservoirs of Newcastle disease virus (NDV). In this study, six Newcastle disease virus strains were isolated and characterized in Tibetan chickens. The HN gene was sequenced, and phylogenetic relationship to reference strains was studied. The phylogenetic analysis demonstrated that these six isolated strains were closely related to NDV isolates of the reference strains GQ245823, KT002186, KU527561, KJ563939, AY225110, EU305607, KM056357, Y18898, GQ245832, AF077761 and lasota strain. Among them, EU305607, KJ563939 and KM056357 were isolated from India, while lasota strain came from attenuated vaccine widely used in China. Then, mean death time (MDT) and intracerebral pathogenicity index (ICPI) were used to estimate the pathogenicity of the isolates. Pathogenicity experiment showed HNH1 and HN17 to be virulent. Our results indicated that genetically diverse viruses circulate in Tibetan chickens, and based upon the phlogeographic analysis, we estimated the origin of ancestral viruses of the isolates and its sister strains located in India and China (lasota strain). It indicates the importance of continuous surveillance to enhance current understanding of the genetic evolution of the NDV strains.
publisher Colégio Brasileiro de Patologia Animal - CBPA
publishDate 2018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000100037
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AT zhanghui phylogeneticandpathotypiccharacterizationofnewcastlediseasevirusintibetanchickenschina
AT wangyajing phylogeneticandpathotypiccharacterizationofnewcastlediseasevirusintibetanchickenschina
AT cirendanba phylogeneticandpathotypiccharacterizationofnewcastlediseasevirusintibetanchickenschina
AT donghailong phylogeneticandpathotypiccharacterizationofnewcastlediseasevirusintibetanchickenschina
AT wuqingxia phylogeneticandpathotypiccharacterizationofnewcastlediseasevirusintibetanchickenschina
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AT nabifazul phylogeneticandpathotypiccharacterizationofnewcastlediseasevirusintibetanchickenschina
AT mehmoodkhalid phylogeneticandpathotypiccharacterizationofnewcastlediseasevirusintibetanchickenschina
AT lijiakui phylogeneticandpathotypiccharacterizationofnewcastlediseasevirusintibetanchickenschina
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