Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil

ABSTRACT: Since the first isolation of canine parvovirus type 2 (CPV-2) in late 70’s new virus types as CPV-2a and CPV-2b have been emerged and becoming prevalent in natural canine population and more recently, a third subtype was identified , CPV-2c. The main purpose of this study was to detect and characterize canine parvovirus currently present in Central-West region of São Paulo state, in Brazil. Fecal samples were collected of vaccinated and non-vaccinated dogs, clinically suspected of having CPV infection brought to the Infectious Diseases Service, Veterinary Hospital of FMVZ-UNESP. All samples (n=30) were screening for canine parvovirus through hemagglutination test and those resulting as positive (n=20) were submitted to PCR and the products were subsequently sequenced for subtype characterization. Results were tested for association with age, hematological values, viral hemagglutination titers in the feces, vaccination status and survival. Leukopenia was found in all animals, death occurred in 30% of unvaccinated dogs and in 42% of vaccinated ones. In a total of 20 positive sequenced samples, 18 were classified as CPV-2b, one as CPV-2c, and one as CPV-2a, being CPV2a and CPV2c detected in unvaccinated puppies. Compared to the reference samples amino acid change at position 426 in those circling virus was identified. The study results demonstrate the predominance of CPV-2b and the presence of CPV-2a and CPV-2c in naturally infected, vaccinated and unvaccinated dogs in in São Paulo region.

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Main Authors: Monteiro,Kyssia, Allendorf,Susan D., Vicente,Acácia F., Appolinário,Camila M., Peres,Marina G., Cortez,Adriana, Heinemann,Marcos B., Megid,Jane
Format: Digital revista
Language:English
Published: Colégio Brasileiro de Patologia Animal - CBPA 2016
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016001201181
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spelling oai:scielo:S0100-736X20160012011812017-03-02Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, BrazilMonteiro,KyssiaAllendorf,Susan D.Vicente,Acácia F.Appolinário,Camila M.Peres,Marina G.Cortez,AdrianaHeinemann,Marcos B.Megid,Jane Canine parvovirus PCR viral type dogs. ABSTRACT: Since the first isolation of canine parvovirus type 2 (CPV-2) in late 70’s new virus types as CPV-2a and CPV-2b have been emerged and becoming prevalent in natural canine population and more recently, a third subtype was identified , CPV-2c. The main purpose of this study was to detect and characterize canine parvovirus currently present in Central-West region of São Paulo state, in Brazil. Fecal samples were collected of vaccinated and non-vaccinated dogs, clinically suspected of having CPV infection brought to the Infectious Diseases Service, Veterinary Hospital of FMVZ-UNESP. All samples (n=30) were screening for canine parvovirus through hemagglutination test and those resulting as positive (n=20) were submitted to PCR and the products were subsequently sequenced for subtype characterization. Results were tested for association with age, hematological values, viral hemagglutination titers in the feces, vaccination status and survival. Leukopenia was found in all animals, death occurred in 30% of unvaccinated dogs and in 42% of vaccinated ones. In a total of 20 positive sequenced samples, 18 were classified as CPV-2b, one as CPV-2c, and one as CPV-2a, being CPV2a and CPV2c detected in unvaccinated puppies. Compared to the reference samples amino acid change at position 426 in those circling virus was identified. The study results demonstrate the predominance of CPV-2b and the presence of CPV-2a and CPV-2c in naturally infected, vaccinated and unvaccinated dogs in in São Paulo region.info:eu-repo/semantics/openAccessColégio Brasileiro de Patologia Animal - CBPAPesquisa Veterinária Brasileira v.36 n.12 20162016-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016001201181en10.1590/s0100-736x2016001200007
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country Brasil
countrycode BR
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libraryname SciELO
language English
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author Monteiro,Kyssia
Allendorf,Susan D.
Vicente,Acácia F.
Appolinário,Camila M.
Peres,Marina G.
Cortez,Adriana
Heinemann,Marcos B.
Megid,Jane
spellingShingle Monteiro,Kyssia
Allendorf,Susan D.
Vicente,Acácia F.
Appolinário,Camila M.
Peres,Marina G.
Cortez,Adriana
Heinemann,Marcos B.
Megid,Jane
Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil
author_facet Monteiro,Kyssia
Allendorf,Susan D.
Vicente,Acácia F.
Appolinário,Camila M.
Peres,Marina G.
Cortez,Adriana
Heinemann,Marcos B.
Megid,Jane
author_sort Monteiro,Kyssia
title Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil
title_short Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil
title_full Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil
title_fullStr Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil
title_full_unstemmed Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil
title_sort viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in são paulo state, brazil
description ABSTRACT: Since the first isolation of canine parvovirus type 2 (CPV-2) in late 70’s new virus types as CPV-2a and CPV-2b have been emerged and becoming prevalent in natural canine population and more recently, a third subtype was identified , CPV-2c. The main purpose of this study was to detect and characterize canine parvovirus currently present in Central-West region of São Paulo state, in Brazil. Fecal samples were collected of vaccinated and non-vaccinated dogs, clinically suspected of having CPV infection brought to the Infectious Diseases Service, Veterinary Hospital of FMVZ-UNESP. All samples (n=30) were screening for canine parvovirus through hemagglutination test and those resulting as positive (n=20) were submitted to PCR and the products were subsequently sequenced for subtype characterization. Results were tested for association with age, hematological values, viral hemagglutination titers in the feces, vaccination status and survival. Leukopenia was found in all animals, death occurred in 30% of unvaccinated dogs and in 42% of vaccinated ones. In a total of 20 positive sequenced samples, 18 were classified as CPV-2b, one as CPV-2c, and one as CPV-2a, being CPV2a and CPV2c detected in unvaccinated puppies. Compared to the reference samples amino acid change at position 426 in those circling virus was identified. The study results demonstrate the predominance of CPV-2b and the presence of CPV-2a and CPV-2c in naturally infected, vaccinated and unvaccinated dogs in in São Paulo region.
publisher Colégio Brasileiro de Patologia Animal - CBPA
publishDate 2016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016001201181
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