Oxidative metabolism and muscle biochemical profile of polo horses supplemented with an ADE vitamin complex

Horses used for the game of polo experience abrupt and frequent changes in exercise intensity. To meet this variable energy demand, the horses use both aerobic and anaerobic pathways in varying proportions and intensities. In this context, there must be a balance between the formation of reactive oxygen species (ROS) and the action of antioxidants to prevent oxidative stress and its consequences. The effect of supplementation with an ADE vitamin complex on oxidative metabolism was evaluated in 18 crossbred horses randomly divided between a treated group (TG) and a control group (CG). The TG animals received the ADE vitamin complex (1mL/50 kg of body weight) by deep intramuscular injection at 30 and 15 days before the game. The CG horses received 10ml of saline by the same administration route and schedule. During the polo match, the animals played for a total of 7.5 min. Blood samples were collected on the same days as the treatments were administered, and immediately before and at 15, 90 and 180 minutes after the game. The concentrations of creatine phosphokinase (CK), lactate dehydrogenase (LDH), lactate, glucose, aspartate aminotransferase (AST), glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured in the blood samples. After the game, the TG demonstrated higher levels of AST, lactate and glucose than the CG, suggesting more efficient energy use by the treated animals. The higher GSH and lower lactate levels in the TG before the game suggest the presence of a greater antioxidant supply in the treated animals. The maintenance of the MDA levels indicates that neither of the groups exhibited oxidative stress.

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Main Authors: Weigel,Rebeca A., Lima,Alessandra Silva, Morgado,Aline Alberti, Nunes,Giovanna Rocha, Miriam,Maurício M., Hagen,Stefano F., Fernandes,Wilson R., Sucupira,Maria C.A.
Format: Digital revista
Language:English
Published: Colégio Brasileiro de Patologia Animal - CBPA 2013
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2013001300010
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spelling oai:scielo:S0100-736X20130013000102014-04-07Oxidative metabolism and muscle biochemical profile of polo horses supplemented with an ADE vitamin complexWeigel,Rebeca A.Lima,Alessandra SilvaMorgado,Aline AlbertiNunes,Giovanna RochaMiriam,Maurício M.Hagen,Stefano F.Fernandes,Wilson R.Sucupira,Maria C.A. ADE vitamin complex oxidative metabolism muscle biochemical profile polo Equidae Horses used for the game of polo experience abrupt and frequent changes in exercise intensity. To meet this variable energy demand, the horses use both aerobic and anaerobic pathways in varying proportions and intensities. In this context, there must be a balance between the formation of reactive oxygen species (ROS) and the action of antioxidants to prevent oxidative stress and its consequences. The effect of supplementation with an ADE vitamin complex on oxidative metabolism was evaluated in 18 crossbred horses randomly divided between a treated group (TG) and a control group (CG). The TG animals received the ADE vitamin complex (1mL/50 kg of body weight) by deep intramuscular injection at 30 and 15 days before the game. The CG horses received 10ml of saline by the same administration route and schedule. During the polo match, the animals played for a total of 7.5 min. Blood samples were collected on the same days as the treatments were administered, and immediately before and at 15, 90 and 180 minutes after the game. The concentrations of creatine phosphokinase (CK), lactate dehydrogenase (LDH), lactate, glucose, aspartate aminotransferase (AST), glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured in the blood samples. After the game, the TG demonstrated higher levels of AST, lactate and glucose than the CG, suggesting more efficient energy use by the treated animals. The higher GSH and lower lactate levels in the TG before the game suggest the presence of a greater antioxidant supply in the treated animals. The maintenance of the MDA levels indicates that neither of the groups exhibited oxidative stress.info:eu-repo/semantics/openAccessColégio Brasileiro de Patologia Animal - CBPAPesquisa Veterinária Brasileira v.33 suppl.1 20132013-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2013001300010en10.1590/S0100-736X2013001300010
institution SCIELO
collection OJS
country Brasil
countrycode BR
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access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Weigel,Rebeca A.
Lima,Alessandra Silva
Morgado,Aline Alberti
Nunes,Giovanna Rocha
Miriam,Maurício M.
Hagen,Stefano F.
Fernandes,Wilson R.
Sucupira,Maria C.A.
spellingShingle Weigel,Rebeca A.
Lima,Alessandra Silva
Morgado,Aline Alberti
Nunes,Giovanna Rocha
Miriam,Maurício M.
Hagen,Stefano F.
Fernandes,Wilson R.
Sucupira,Maria C.A.
Oxidative metabolism and muscle biochemical profile of polo horses supplemented with an ADE vitamin complex
author_facet Weigel,Rebeca A.
Lima,Alessandra Silva
Morgado,Aline Alberti
Nunes,Giovanna Rocha
Miriam,Maurício M.
Hagen,Stefano F.
Fernandes,Wilson R.
Sucupira,Maria C.A.
author_sort Weigel,Rebeca A.
title Oxidative metabolism and muscle biochemical profile of polo horses supplemented with an ADE vitamin complex
title_short Oxidative metabolism and muscle biochemical profile of polo horses supplemented with an ADE vitamin complex
title_full Oxidative metabolism and muscle biochemical profile of polo horses supplemented with an ADE vitamin complex
title_fullStr Oxidative metabolism and muscle biochemical profile of polo horses supplemented with an ADE vitamin complex
title_full_unstemmed Oxidative metabolism and muscle biochemical profile of polo horses supplemented with an ADE vitamin complex
title_sort oxidative metabolism and muscle biochemical profile of polo horses supplemented with an ade vitamin complex
description Horses used for the game of polo experience abrupt and frequent changes in exercise intensity. To meet this variable energy demand, the horses use both aerobic and anaerobic pathways in varying proportions and intensities. In this context, there must be a balance between the formation of reactive oxygen species (ROS) and the action of antioxidants to prevent oxidative stress and its consequences. The effect of supplementation with an ADE vitamin complex on oxidative metabolism was evaluated in 18 crossbred horses randomly divided between a treated group (TG) and a control group (CG). The TG animals received the ADE vitamin complex (1mL/50 kg of body weight) by deep intramuscular injection at 30 and 15 days before the game. The CG horses received 10ml of saline by the same administration route and schedule. During the polo match, the animals played for a total of 7.5 min. Blood samples were collected on the same days as the treatments were administered, and immediately before and at 15, 90 and 180 minutes after the game. The concentrations of creatine phosphokinase (CK), lactate dehydrogenase (LDH), lactate, glucose, aspartate aminotransferase (AST), glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured in the blood samples. After the game, the TG demonstrated higher levels of AST, lactate and glucose than the CG, suggesting more efficient energy use by the treated animals. The higher GSH and lower lactate levels in the TG before the game suggest the presence of a greater antioxidant supply in the treated animals. The maintenance of the MDA levels indicates that neither of the groups exhibited oxidative stress.
publisher Colégio Brasileiro de Patologia Animal - CBPA
publishDate 2013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2013001300010
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