The influence of nicotine in healing of small bowel anastomoses in rats: angiogenesis and miofibroblasts
ABSTRACT Objective: to know the effect of nicotine on angiogenesis and myofibroblast formation in anastomoses of the small bowel of rats. Methods: we randomly divided 60 Wistar rats into the groups Nicotine (N) and control (C), according to the proposed treatment. Each group was subdivided into three subgroups according to the time interval used for the evaluation (7, 14 or 28 days). The N group with 30 animals received nicotine subcutaneously at a dose of 2mg/kg body weight, diluted in 0.3ml of 0.9% saline, twice daily for 28 days prior to the operation, and for more 7, 14 or 28 days, depending on the subgroup. The C group (also 30 animals) received only saline on the same conditions and time intervals. After 28 days we carried out an end-to-end anastomosis 10cm distal to the duodenojejunal flexure in each rat. After 7, 14 or 28 days after surgery, we euthanized ten animals of each group, sent specimens of the anastomosis areas, 1cm proximal to 1cm distal, to counting of blood vessels and myofibroblasts through immunohistochemical staining by the application of monoclonal anti-factor VIII antibodies and anti-smooth muscle alpha-actin. Results: the administration of nicotine led to the decrease in the number of blood vessels measured on the 28th postoperative day and the number of myofibroblasts measured on the seventh day following completion of the anastomoses. Conclusion: administration of nicotine was deleterious on angiogenesis and myofibroblast formation in rats' small intestine anastomoses.
Main Authors: | , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Colégio Brasileiro de Cirurgiões
2016
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912016000200087 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0100-69912016000200087 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0100-699120160002000872016-05-25The influence of nicotine in healing of small bowel anastomoses in rats: angiogenesis and miofibroblastsSkinovsky,JamesMalafaia,OsvaldoChibata,MauricioTsumanuma,FernandaPanegalli Filho,FlávioMartins,Marcus Vinícius Dantas de Campos Wound Healing. Nicotine. Intestine Small. Anastomosis Surgical. Rats. ABSTRACT Objective: to know the effect of nicotine on angiogenesis and myofibroblast formation in anastomoses of the small bowel of rats. Methods: we randomly divided 60 Wistar rats into the groups Nicotine (N) and control (C), according to the proposed treatment. Each group was subdivided into three subgroups according to the time interval used for the evaluation (7, 14 or 28 days). The N group with 30 animals received nicotine subcutaneously at a dose of 2mg/kg body weight, diluted in 0.3ml of 0.9% saline, twice daily for 28 days prior to the operation, and for more 7, 14 or 28 days, depending on the subgroup. The C group (also 30 animals) received only saline on the same conditions and time intervals. After 28 days we carried out an end-to-end anastomosis 10cm distal to the duodenojejunal flexure in each rat. After 7, 14 or 28 days after surgery, we euthanized ten animals of each group, sent specimens of the anastomosis areas, 1cm proximal to 1cm distal, to counting of blood vessels and myofibroblasts through immunohistochemical staining by the application of monoclonal anti-factor VIII antibodies and anti-smooth muscle alpha-actin. Results: the administration of nicotine led to the decrease in the number of blood vessels measured on the 28th postoperative day and the number of myofibroblasts measured on the seventh day following completion of the anastomoses. Conclusion: administration of nicotine was deleterious on angiogenesis and myofibroblast formation in rats' small intestine anastomoses.info:eu-repo/semantics/openAccessColégio Brasileiro de CirurgiõesRevista do Colégio Brasileiro de Cirurgiões v.43 n.2 20162016-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912016000200087en10.1590/0100-69912016002004 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Skinovsky,James Malafaia,Osvaldo Chibata,Mauricio Tsumanuma,Fernanda Panegalli Filho,Flávio Martins,Marcus Vinícius Dantas de Campos |
spellingShingle |
Skinovsky,James Malafaia,Osvaldo Chibata,Mauricio Tsumanuma,Fernanda Panegalli Filho,Flávio Martins,Marcus Vinícius Dantas de Campos The influence of nicotine in healing of small bowel anastomoses in rats: angiogenesis and miofibroblasts |
author_facet |
Skinovsky,James Malafaia,Osvaldo Chibata,Mauricio Tsumanuma,Fernanda Panegalli Filho,Flávio Martins,Marcus Vinícius Dantas de Campos |
author_sort |
Skinovsky,James |
title |
The influence of nicotine in healing of small bowel anastomoses in rats: angiogenesis and miofibroblasts |
title_short |
The influence of nicotine in healing of small bowel anastomoses in rats: angiogenesis and miofibroblasts |
title_full |
The influence of nicotine in healing of small bowel anastomoses in rats: angiogenesis and miofibroblasts |
title_fullStr |
The influence of nicotine in healing of small bowel anastomoses in rats: angiogenesis and miofibroblasts |
title_full_unstemmed |
The influence of nicotine in healing of small bowel anastomoses in rats: angiogenesis and miofibroblasts |
title_sort |
influence of nicotine in healing of small bowel anastomoses in rats: angiogenesis and miofibroblasts |
description |
ABSTRACT Objective: to know the effect of nicotine on angiogenesis and myofibroblast formation in anastomoses of the small bowel of rats. Methods: we randomly divided 60 Wistar rats into the groups Nicotine (N) and control (C), according to the proposed treatment. Each group was subdivided into three subgroups according to the time interval used for the evaluation (7, 14 or 28 days). The N group with 30 animals received nicotine subcutaneously at a dose of 2mg/kg body weight, diluted in 0.3ml of 0.9% saline, twice daily for 28 days prior to the operation, and for more 7, 14 or 28 days, depending on the subgroup. The C group (also 30 animals) received only saline on the same conditions and time intervals. After 28 days we carried out an end-to-end anastomosis 10cm distal to the duodenojejunal flexure in each rat. After 7, 14 or 28 days after surgery, we euthanized ten animals of each group, sent specimens of the anastomosis areas, 1cm proximal to 1cm distal, to counting of blood vessels and myofibroblasts through immunohistochemical staining by the application of monoclonal anti-factor VIII antibodies and anti-smooth muscle alpha-actin. Results: the administration of nicotine led to the decrease in the number of blood vessels measured on the 28th postoperative day and the number of myofibroblasts measured on the seventh day following completion of the anastomoses. Conclusion: administration of nicotine was deleterious on angiogenesis and myofibroblast formation in rats' small intestine anastomoses. |
publisher |
Colégio Brasileiro de Cirurgiões |
publishDate |
2016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912016000200087 |
work_keys_str_mv |
AT skinovskyjames theinfluenceofnicotineinhealingofsmallbowelanastomosesinratsangiogenesisandmiofibroblasts AT malafaiaosvaldo theinfluenceofnicotineinhealingofsmallbowelanastomosesinratsangiogenesisandmiofibroblasts AT chibatamauricio theinfluenceofnicotineinhealingofsmallbowelanastomosesinratsangiogenesisandmiofibroblasts AT tsumanumafernanda theinfluenceofnicotineinhealingofsmallbowelanastomosesinratsangiogenesisandmiofibroblasts AT panegallifilhoflavio theinfluenceofnicotineinhealingofsmallbowelanastomosesinratsangiogenesisandmiofibroblasts AT martinsmarcusviniciusdantasdecampos theinfluenceofnicotineinhealingofsmallbowelanastomosesinratsangiogenesisandmiofibroblasts AT skinovskyjames influenceofnicotineinhealingofsmallbowelanastomosesinratsangiogenesisandmiofibroblasts AT malafaiaosvaldo influenceofnicotineinhealingofsmallbowelanastomosesinratsangiogenesisandmiofibroblasts AT chibatamauricio influenceofnicotineinhealingofsmallbowelanastomosesinratsangiogenesisandmiofibroblasts AT tsumanumafernanda influenceofnicotineinhealingofsmallbowelanastomosesinratsangiogenesisandmiofibroblasts AT panegallifilhoflavio influenceofnicotineinhealingofsmallbowelanastomosesinratsangiogenesisandmiofibroblasts AT martinsmarcusviniciusdantasdecampos influenceofnicotineinhealingofsmallbowelanastomosesinratsangiogenesisandmiofibroblasts |
_version_ |
1756389349980635136 |