Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
Pothomorphe umbellata (L.) known on Brazil as Caapeba has a number of popular medicinal use, and it has been studied in relation to its pharmacological activity. Peroxidase specific activity (units/mg protein) was evaluated in callus cell culture samples of the P.umbellata, grown in two different MS medium (media 1 and media 2), submitted to 16 hours photoperiod or kept in darkness. Cell growth rate curve showed that the best growth indices were observed when media 2 submitted to the photoperiod regime was used, followed by the same media kept in darkness (stress condition). The results obtained also showed that the cell culture grown under stress conditions (darkness) lead to high content of peroxidase enzyme (an increase of 700% was observed). Kinetic constant values of 3.3 mmol.L-1 and 2,8 sec-1 were obtained for kM and v max,, respectively, using guaiacol as enzyme substrate.
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Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP
2006
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oai:scielo:S0100-467020060003000082006-12-14Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzymeKhalil,N. M.Mello,M. A. M.França,S. C.Oliveira,L. A. A.Oliveira,O. M. M. F. Pothomorphe umbellata (L.) cell culture peroxidase Pothomorphe umbellata (L.) known on Brazil as Caapeba has a number of popular medicinal use, and it has been studied in relation to its pharmacological activity. Peroxidase specific activity (units/mg protein) was evaluated in callus cell culture samples of the P.umbellata, grown in two different MS medium (media 1 and media 2), submitted to 16 hours photoperiod or kept in darkness. Cell growth rate curve showed that the best growth indices were observed when media 2 submitted to the photoperiod regime was used, followed by the same media kept in darkness (stress condition). The results obtained also showed that the cell culture grown under stress conditions (darkness) lead to high content of peroxidase enzyme (an increase of 700% was observed). Kinetic constant values of 3.3 mmol.L-1 and 2,8 sec-1 were obtained for kM and v max,, respectively, using guaiacol as enzyme substrate.info:eu-repo/semantics/openAccessFundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESPEclética Química v.31 n.3 20062006-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-46702006000300008en10.1590/S0100-46702006000300008 |
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| author |
Khalil,N. M. Mello,M. A. M. França,S. C. Oliveira,L. A. A. Oliveira,O. M. M. F. |
| spellingShingle |
Khalil,N. M. Mello,M. A. M. França,S. C. Oliveira,L. A. A. Oliveira,O. M. M. F. Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme |
| author_facet |
Khalil,N. M. Mello,M. A. M. França,S. C. Oliveira,L. A. A. Oliveira,O. M. M. F. |
| author_sort |
Khalil,N. M. |
| title |
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme |
| title_short |
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme |
| title_full |
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme |
| title_fullStr |
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme |
| title_full_unstemmed |
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme |
| title_sort |
callus cell culture of pothomorphe umbellata (l.) under stress condition leads to high content of peroxidase enzyme |
| description |
Pothomorphe umbellata (L.) known on Brazil as Caapeba has a number of popular medicinal use, and it has been studied in relation to its pharmacological activity. Peroxidase specific activity (units/mg protein) was evaluated in callus cell culture samples of the P.umbellata, grown in two different MS medium (media 1 and media 2), submitted to 16 hours photoperiod or kept in darkness. Cell growth rate curve showed that the best growth indices were observed when media 2 submitted to the photoperiod regime was used, followed by the same media kept in darkness (stress condition). The results obtained also showed that the cell culture grown under stress conditions (darkness) lead to high content of peroxidase enzyme (an increase of 700% was observed). Kinetic constant values of 3.3 mmol.L-1 and 2,8 sec-1 were obtained for kM and v max,, respectively, using guaiacol as enzyme substrate. |
| publisher |
Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP |
| publishDate |
2006 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-46702006000300008 |
| work_keys_str_mv |
AT khalilnm calluscellcultureofpothomorpheumbellatalunderstressconditionleadstohighcontentofperoxidaseenzyme AT mellomam calluscellcultureofpothomorpheumbellatalunderstressconditionleadstohighcontentofperoxidaseenzyme AT francasc calluscellcultureofpothomorpheumbellatalunderstressconditionleadstohighcontentofperoxidaseenzyme AT oliveiralaa calluscellcultureofpothomorpheumbellatalunderstressconditionleadstohighcontentofperoxidaseenzyme AT oliveiraommf calluscellcultureofpothomorpheumbellatalunderstressconditionleadstohighcontentofperoxidaseenzyme |
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1756387927564222464 |