Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil
A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR.
Main Authors: | , , , , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Fitopatologia
2005
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400005 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0100-41582005000400005 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0100-415820050004000052005-10-17Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in BrazilAlmeida,Álvaro M. R.Sakai,JunichiHanada,KaoruOliveira,Thalita G.Belintani,PriscilaKitajima,Elliot W.Souto,Eliezer R.Novaes,Tanara G. deNora,Paulo S. nucleotide sequence Elisa host range A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR.info:eu-repo/semantics/openAccessSociedade Brasileira de FitopatologiaFitopatologia Brasileira v.30 n.4 20052005-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400005en10.1590/S0100-41582005000400005 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Almeida,Álvaro M. R. Sakai,Junichi Hanada,Kaoru Oliveira,Thalita G. Belintani,Priscila Kitajima,Elliot W. Souto,Eliezer R. Novaes,Tanara G. de Nora,Paulo S. |
spellingShingle |
Almeida,Álvaro M. R. Sakai,Junichi Hanada,Kaoru Oliveira,Thalita G. Belintani,Priscila Kitajima,Elliot W. Souto,Eliezer R. Novaes,Tanara G. de Nora,Paulo S. Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil |
author_facet |
Almeida,Álvaro M. R. Sakai,Junichi Hanada,Kaoru Oliveira,Thalita G. Belintani,Priscila Kitajima,Elliot W. Souto,Eliezer R. Novaes,Tanara G. de Nora,Paulo S. |
author_sort |
Almeida,Álvaro M. R. |
title |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil |
title_short |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil |
title_full |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil |
title_fullStr |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil |
title_full_unstemmed |
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil |
title_sort |
biological and molecular characterization of an isolate of tobacco streak virus obtained from soybeans in brazil |
description |
A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR. |
publisher |
Sociedade Brasileira de Fitopatologia |
publishDate |
2005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400005 |
work_keys_str_mv |
AT almeidaalvaromr biologicalandmolecularcharacterizationofanisolateoftobaccostreakvirusobtainedfromsoybeansinbrazil AT sakaijunichi biologicalandmolecularcharacterizationofanisolateoftobaccostreakvirusobtainedfromsoybeansinbrazil AT hanadakaoru biologicalandmolecularcharacterizationofanisolateoftobaccostreakvirusobtainedfromsoybeansinbrazil AT oliveirathalitag biologicalandmolecularcharacterizationofanisolateoftobaccostreakvirusobtainedfromsoybeansinbrazil AT belintanipriscila biologicalandmolecularcharacterizationofanisolateoftobaccostreakvirusobtainedfromsoybeansinbrazil AT kitajimaelliotw biologicalandmolecularcharacterizationofanisolateoftobaccostreakvirusobtainedfromsoybeansinbrazil AT soutoeliezerr biologicalandmolecularcharacterizationofanisolateoftobaccostreakvirusobtainedfromsoybeansinbrazil AT novaestanaragde biologicalandmolecularcharacterizationofanisolateoftobaccostreakvirusobtainedfromsoybeansinbrazil AT norapaulos biologicalandmolecularcharacterizationofanisolateoftobaccostreakvirusobtainedfromsoybeansinbrazil |
_version_ |
1756387851346378752 |