Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil

A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR.

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Main Authors: Almeida,Álvaro M. R., Sakai,Junichi, Hanada,Kaoru, Oliveira,Thalita G., Belintani,Priscila, Kitajima,Elliot W., Souto,Eliezer R., Novaes,Tanara G. de, Nora,Paulo S.
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Fitopatologia 2005
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400005
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spelling oai:scielo:S0100-415820050004000052005-10-17Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in BrazilAlmeida,Álvaro M. R.Sakai,JunichiHanada,KaoruOliveira,Thalita G.Belintani,PriscilaKitajima,Elliot W.Souto,Eliezer R.Novaes,Tanara G. deNora,Paulo S. nucleotide sequence Elisa host range A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR.info:eu-repo/semantics/openAccessSociedade Brasileira de FitopatologiaFitopatologia Brasileira v.30 n.4 20052005-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400005en10.1590/S0100-41582005000400005
institution SCIELO
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country Brasil
countrycode BR
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region America del Sur
libraryname SciELO
language English
format Digital
author Almeida,Álvaro M. R.
Sakai,Junichi
Hanada,Kaoru
Oliveira,Thalita G.
Belintani,Priscila
Kitajima,Elliot W.
Souto,Eliezer R.
Novaes,Tanara G. de
Nora,Paulo S.
spellingShingle Almeida,Álvaro M. R.
Sakai,Junichi
Hanada,Kaoru
Oliveira,Thalita G.
Belintani,Priscila
Kitajima,Elliot W.
Souto,Eliezer R.
Novaes,Tanara G. de
Nora,Paulo S.
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil
author_facet Almeida,Álvaro M. R.
Sakai,Junichi
Hanada,Kaoru
Oliveira,Thalita G.
Belintani,Priscila
Kitajima,Elliot W.
Souto,Eliezer R.
Novaes,Tanara G. de
Nora,Paulo S.
author_sort Almeida,Álvaro M. R.
title Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil
title_short Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil
title_full Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil
title_fullStr Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil
title_full_unstemmed Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil
title_sort biological and molecular characterization of an isolate of tobacco streak virus obtained from soybeans in brazil
description A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR.
publisher Sociedade Brasileira de Fitopatologia
publishDate 2005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400005
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