CARACTERIZAÇÃO E CONTROLE DE QUALIDADE DE ÓLEOS DE COPAÍBA (COPAIFERA SP.) UTILIZANDO DETECÇÃO DE MARCADORES POR FATOR DE RETENÇÃO RELATIVA EM HPTLC
Copaiba oilresins present medicinal uses that demand the development of methodologies that could ensure the authenticity and guarantee the species differentiation. The usual way to differentiate the copaiba oils is analyze the terpene composition, mainly the diterpenic acids profile. Unlike caryophyllene and its oxide, copaiba diterpenes are not commercially available and lab obtention is expensive and time consuming. At this paper, a new methodology is presented that allows the fast and reliable detection of copaiba biomarkers using HPTLC retention factor relative to an appropriate standard. Several commercially available substances were analyzed and the monoterpene linalool oxide was chosen. The comparison of relative retention factors allowed the confirmation of caryophyllene and its oxides and the diterpenic acids copalic, hydroxy-copalic, acetoxy-copalic, agathic, and hardwickiic. Calibration curve showed strong correlation (│r│ ≥0.9962) with R2 ≥0.9928.
Main Authors: | Lima,Milena C. F. de, Cavalcante,Samir F. de Almeida, Wiedemann,Larissa S. M., Veiga Junior,Valdir F. da |
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Format: | Digital revista |
Language: | Portuguese |
Published: |
Sociedade Brasileira de Química
2020
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Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000700878 |
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