AVALIAÇÃO DA BIOMASSA DE FUNGOS AMAZÔNICOS COMO FONTE DE LIPASES PARA BIOCATÁLISE
We evaluated the biomass of twenty Amazonian fungal isolates as a potential source of mycelium-bound lipases with hydrolytic, synthetic or enantioselective activity for biocatalysis application. We compared the hydrolytic activity of three biomass treatments (delipidated, non-delipidated and cultivated in medium without inducer). Delipidated biomass showed better results in the hydrolysis of p-nitrophenyl palmitate compared to the other two treatments for fifteen isolates. Delipidated biomass of six Aspergillus strains and UEA_115 strain showed a high synthesis activity of ethyl palmitate by transesterification in organic medium. Results were confirmed by spectrophotometry (410 nm) and gas chromatography. In this reaction, the isolate DPUA_1539 A. flavo-furcatis reached a maximum value of 668.5 ± 23.5 mU g-1. Enantioselective activity assays indicated that biomass-bound lipases from UEA_115 isolate (E = 3.58; ees = 7 ± 0) and in particular from the DPUA_1539 A. flavo-furcatis isolate (E = 24.15; ees = 91 ± 1) have the ability to discriminate enantiomers of the drug ibuprofen by ester synthesis, preferably with (R)-enantiomer. These results encourage further investigations of these fungi as potential lipase suppliers for biocatalytic processes such as biodiesel production and enantiopure drugs.
Main Authors: | Romano,Israel P., Santos,Vanderlei S. dos, Louzada,Ana Carolina de Lima Paes, Pereira Junior,Raimundo C., Carmo,Edson J. do, Mota,Adolfo José da, Barroso,Hiléia dos S., Itabaiana Junior,Ivaldo, Pereira,José Odair, Astolfi Filho,Spartaco, Zanotto,Sandra Patrícia |
---|---|
Format: | Digital revista |
Language: | Portuguese |
Published: |
Sociedade Brasileira de Química
2020
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000200146 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Potencial de biocatálise enantiosseletiva de lipases microbianas
by: Carvalho,Patrícia de O., et al.
Published: (2005) -
Biocatálise: avanços recentes
by: De Conti,Roseli, et al.
Published: (2001) -
Fatos e tendências da biocatálise
by: Gonçalves,Caroline da C. S., et al.
Published: (2013) -
Biocatálise heterogênea em fase sólido/gás: princípios e aplicações
by: Perez,Victor H., et al.
Published: (2014) -
Seleção e imobilização de fungos filamentosos produtores de lipase intracelular.
by: SILVA, G. dos S., et al.
Published: (2009-11-19)