Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free
Candida antarctica lipase B (CALB) is an enzyme able to catalyze chemical reaction, however when it is used as a free enzyme, it cannot be recovered from reaction medium. One of the alternatives is to immobilize the enzymes on a support which allows the maintenance of their catalytic activities. The purpose of this paper was to immobilize the CALB on MCM-48 using the ionic solid [C16MI]Cl as structure director. 22 CCRD (Central Composite Rotational Design) was proposed to analyze the influence of the variables like enzyme mass (0.059 to 0.341 g) and ionic solid concentration (0.59 to 3.41%) in the enzyme immobilization process to obtain the maximum esterification activity in order to optimize the process. After immobilization, the study results showed that the enzymes exhibited improvement of thermal (40, 60 and 80 ºC) and storage stability (90 days), besides the possibility to reuse of the enzyme up to 10 times, showing residual activity of 50%.
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Sociedade Brasileira de Química
2017
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oai:scielo:S0100-404220170003002932017-05-05Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-freeBattiston,Catia S. Z.Ficanha,Aline M. M.Levandoski,Katarine L. D.Silva,Bernardo A. daBattiston,SuellenDallago,Rogério M.Mignoni,Marcelo L. lipase immobilization MCM-48 ionic solid Candida antarctica lipase B (CALB) is an enzyme able to catalyze chemical reaction, however when it is used as a free enzyme, it cannot be recovered from reaction medium. One of the alternatives is to immobilize the enzymes on a support which allows the maintenance of their catalytic activities. The purpose of this paper was to immobilize the CALB on MCM-48 using the ionic solid [C16MI]Cl as structure director. 22 CCRD (Central Composite Rotational Design) was proposed to analyze the influence of the variables like enzyme mass (0.059 to 0.341 g) and ionic solid concentration (0.59 to 3.41%) in the enzyme immobilization process to obtain the maximum esterification activity in order to optimize the process. After immobilization, the study results showed that the enzymes exhibited improvement of thermal (40, 60 and 80 ºC) and storage stability (90 days), besides the possibility to reuse of the enzyme up to 10 times, showing residual activity of 50%.info:eu-repo/semantics/openAccessSociedade Brasileira de QuímicaQuímica Nova v.40 n.3 20172017-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000300293en10.21577/0100-4042.20170011 |
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Battiston,Catia S. Z. Ficanha,Aline M. M. Levandoski,Katarine L. D. Silva,Bernardo A. da Battiston,Suellen Dallago,Rogério M. Mignoni,Marcelo L. |
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Battiston,Catia S. Z. Ficanha,Aline M. M. Levandoski,Katarine L. D. Silva,Bernardo A. da Battiston,Suellen Dallago,Rogério M. Mignoni,Marcelo L. Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free |
| author_facet |
Battiston,Catia S. Z. Ficanha,Aline M. M. Levandoski,Katarine L. D. Silva,Bernardo A. da Battiston,Suellen Dallago,Rogério M. Mignoni,Marcelo L. |
| author_sort |
Battiston,Catia S. Z. |
| title |
Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free |
| title_short |
Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free |
| title_full |
Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free |
| title_fullStr |
Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free |
| title_full_unstemmed |
Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free |
| title_sort |
immobilization of lipase on mesoporous molecular sieve mcm-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free |
| description |
Candida antarctica lipase B (CALB) is an enzyme able to catalyze chemical reaction, however when it is used as a free enzyme, it cannot be recovered from reaction medium. One of the alternatives is to immobilize the enzymes on a support which allows the maintenance of their catalytic activities. The purpose of this paper was to immobilize the CALB on MCM-48 using the ionic solid [C16MI]Cl as structure director. 22 CCRD (Central Composite Rotational Design) was proposed to analyze the influence of the variables like enzyme mass (0.059 to 0.341 g) and ionic solid concentration (0.59 to 3.41%) in the enzyme immobilization process to obtain the maximum esterification activity in order to optimize the process. After immobilization, the study results showed that the enzymes exhibited improvement of thermal (40, 60 and 80 ºC) and storage stability (90 days), besides the possibility to reuse of the enzyme up to 10 times, showing residual activity of 50%. |
| publisher |
Sociedade Brasileira de Química |
| publishDate |
2017 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000300293 |
| work_keys_str_mv |
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