Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies
A chromatographic technique for determination of rutin and narcissin in marigold extract and topical formulations was developed and validated. The method shows linearity over the concentration range of 0.2 - 6.0 μg/mL of rutin (r = 0.9986) and 0.8 - 12.0 μg/mL of narcissin (r = 0.9951). The values obtained for precision and accuracy are in agreement with ICH guidelines. Both the formulation excipients and the porcine ear skin samples did not interfere with the flavonoids determination. The recovery of rutin and narcissin in skin samples added with marigold extract was 81.41% and 83.35%, respectively, which demonstrate the applicability of this method to perform skin penetration studies.
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Sociedade Brasileira de Química
2010
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oai:scielo:S0100-404220100006000192010-08-24Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studiesFonseca,Yris MariaVicentini,Fabiana T. M. C.Catini,Carolina DiasFonseca,Maria José Vieira Calendula officinalis narcissin rutin A chromatographic technique for determination of rutin and narcissin in marigold extract and topical formulations was developed and validated. The method shows linearity over the concentration range of 0.2 - 6.0 μg/mL of rutin (r = 0.9986) and 0.8 - 12.0 μg/mL of narcissin (r = 0.9951). The values obtained for precision and accuracy are in agreement with ICH guidelines. Both the formulation excipients and the porcine ear skin samples did not interfere with the flavonoids determination. The recovery of rutin and narcissin in skin samples added with marigold extract was 81.41% and 83.35%, respectively, which demonstrate the applicability of this method to perform skin penetration studies.info:eu-repo/semantics/openAccessSociedade Brasileira de QuímicaQuímica Nova v.33 n.6 20102010-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000600019en10.1590/S0100-40422010000600019 |
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Fonseca,Yris Maria Vicentini,Fabiana T. M. C. Catini,Carolina Dias Fonseca,Maria José Vieira |
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Fonseca,Yris Maria Vicentini,Fabiana T. M. C. Catini,Carolina Dias Fonseca,Maria José Vieira Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies |
| author_facet |
Fonseca,Yris Maria Vicentini,Fabiana T. M. C. Catini,Carolina Dias Fonseca,Maria José Vieira |
| author_sort |
Fonseca,Yris Maria |
| title |
Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies |
| title_short |
Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies |
| title_full |
Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies |
| title_fullStr |
Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies |
| title_full_unstemmed |
Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies |
| title_sort |
determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies |
| description |
A chromatographic technique for determination of rutin and narcissin in marigold extract and topical formulations was developed and validated. The method shows linearity over the concentration range of 0.2 - 6.0 μg/mL of rutin (r = 0.9986) and 0.8 - 12.0 μg/mL of narcissin (r = 0.9951). The values obtained for precision and accuracy are in agreement with ICH guidelines. Both the formulation excipients and the porcine ear skin samples did not interfere with the flavonoids determination. The recovery of rutin and narcissin in skin samples added with marigold extract was 81.41% and 83.35%, respectively, which demonstrate the applicability of this method to perform skin penetration studies. |
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Sociedade Brasileira de Química |
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2010 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000600019 |
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