Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café
Epidemiological studies attributed positive effects in the central nervous system (CNS) to coffee. Among possible active constituents, serotonin, a neurotransmitter in the CNS, is present; but dietary sources do not cross the blood-brain barrier. Tryptophan and 5-hidroxytryptophan (5-HTP) are serotonin precursors and can affect brain concentrations. An ion-pair-HPLC, post-column derivatization with o-phthalaldehyde and fluorimetric detection before and after hydrolysis with NaOH and extraction with methanol:water was developed for the simultaneous determination of these compounds. It was selective, sensitive (LOD = 0.3 and 0.2 μg/mL), precise (91.3 and 94.2% recovery for tryptophan and 5-HTP, respectively), and linear from 0.3 to 40 μg/mL for both compounds. It was applied to green and roasted arabica and robusta coffees.
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Sociedade Brasileira de Química
2010
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oai:scielo:S0100-404220100002000162010-03-12Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em caféMartins,Ana Carolina C. L.Silva,Tarliane M.Gloria,M. Beatriz A. coffee serotonin tryptophan Epidemiological studies attributed positive effects in the central nervous system (CNS) to coffee. Among possible active constituents, serotonin, a neurotransmitter in the CNS, is present; but dietary sources do not cross the blood-brain barrier. Tryptophan and 5-hidroxytryptophan (5-HTP) are serotonin precursors and can affect brain concentrations. An ion-pair-HPLC, post-column derivatization with o-phthalaldehyde and fluorimetric detection before and after hydrolysis with NaOH and extraction with methanol:water was developed for the simultaneous determination of these compounds. It was selective, sensitive (LOD = 0.3 and 0.2 μg/mL), precise (91.3 and 94.2% recovery for tryptophan and 5-HTP, respectively), and linear from 0.3 to 40 μg/mL for both compounds. It was applied to green and roasted arabica and robusta coffees.info:eu-repo/semantics/openAccessSociedade Brasileira de QuímicaQuímica Nova v.33 n.2 20102010-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200016pt10.1590/S0100-40422010000200016 |
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author |
Martins,Ana Carolina C. L. Silva,Tarliane M. Gloria,M. Beatriz A. |
spellingShingle |
Martins,Ana Carolina C. L. Silva,Tarliane M. Gloria,M. Beatriz A. Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café |
author_facet |
Martins,Ana Carolina C. L. Silva,Tarliane M. Gloria,M. Beatriz A. |
author_sort |
Martins,Ana Carolina C. L. |
title |
Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café |
title_short |
Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café |
title_full |
Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café |
title_fullStr |
Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café |
title_full_unstemmed |
Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café |
title_sort |
determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café |
description |
Epidemiological studies attributed positive effects in the central nervous system (CNS) to coffee. Among possible active constituents, serotonin, a neurotransmitter in the CNS, is present; but dietary sources do not cross the blood-brain barrier. Tryptophan and 5-hidroxytryptophan (5-HTP) are serotonin precursors and can affect brain concentrations. An ion-pair-HPLC, post-column derivatization with o-phthalaldehyde and fluorimetric detection before and after hydrolysis with NaOH and extraction with methanol:water was developed for the simultaneous determination of these compounds. It was selective, sensitive (LOD = 0.3 and 0.2 μg/mL), precise (91.3 and 94.2% recovery for tryptophan and 5-HTP, respectively), and linear from 0.3 to 40 μg/mL for both compounds. It was applied to green and roasted arabica and robusta coffees. |
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Sociedade Brasileira de Química |
publishDate |
2010 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200016 |
work_keys_str_mv |
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