Un método reproducible para obtener peg biramificado monofuncional de alta pureza
PEGylation has become a widely applied technique to enhancing in vitro and in vivo stability of therapeutic proteins and to increasing materials biocompatibility. PEG branched structures have proven useful for protein and peptide modification. Furthermore, they may be better than linear structures for many purposes. This paper describes an improved procedure for obtaining 2-arms PEG based on L-lysine. The efficiency of the synthesis was not related to moisture of the raw materials. This procedure does not use hazardous reagents as previous protocols do. It implemented a purification process for obtaining the desired structure with high purity ( > 99%). Finally, the procedure described here allows the obtaining of others PEGylation reagents.
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Sociedade Brasileira de Química
2009
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oai:scielo:S0100-404220090006000122009-09-22Un método reproducible para obtener peg biramificado monofuncional de alta purezaRamón,José A.Peniche,CarlosCastro,Fidel R.Sáez,Vivian M.Colás,Rosa M.Páez,Rolando PEGylation branched PEG interferon PEGylation has become a widely applied technique to enhancing in vitro and in vivo stability of therapeutic proteins and to increasing materials biocompatibility. PEG branched structures have proven useful for protein and peptide modification. Furthermore, they may be better than linear structures for many purposes. This paper describes an improved procedure for obtaining 2-arms PEG based on L-lysine. The efficiency of the synthesis was not related to moisture of the raw materials. This procedure does not use hazardous reagents as previous protocols do. It implemented a purification process for obtaining the desired structure with high purity ( > 99%). Finally, the procedure described here allows the obtaining of others PEGylation reagents.info:eu-repo/semantics/openAccessSociedade Brasileira de QuímicaQuímica Nova v.32 n.6 20092009-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000600012es10.1590/S0100-40422009000600012 |
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Ramón,José A. Peniche,Carlos Castro,Fidel R. Sáez,Vivian M. Colás,Rosa M. Páez,Rolando |
spellingShingle |
Ramón,José A. Peniche,Carlos Castro,Fidel R. Sáez,Vivian M. Colás,Rosa M. Páez,Rolando Un método reproducible para obtener peg biramificado monofuncional de alta pureza |
author_facet |
Ramón,José A. Peniche,Carlos Castro,Fidel R. Sáez,Vivian M. Colás,Rosa M. Páez,Rolando |
author_sort |
Ramón,José A. |
title |
Un método reproducible para obtener peg biramificado monofuncional de alta pureza |
title_short |
Un método reproducible para obtener peg biramificado monofuncional de alta pureza |
title_full |
Un método reproducible para obtener peg biramificado monofuncional de alta pureza |
title_fullStr |
Un método reproducible para obtener peg biramificado monofuncional de alta pureza |
title_full_unstemmed |
Un método reproducible para obtener peg biramificado monofuncional de alta pureza |
title_sort |
un método reproducible para obtener peg biramificado monofuncional de alta pureza |
description |
PEGylation has become a widely applied technique to enhancing in vitro and in vivo stability of therapeutic proteins and to increasing materials biocompatibility. PEG branched structures have proven useful for protein and peptide modification. Furthermore, they may be better than linear structures for many purposes. This paper describes an improved procedure for obtaining 2-arms PEG based on L-lysine. The efficiency of the synthesis was not related to moisture of the raw materials. This procedure does not use hazardous reagents as previous protocols do. It implemented a purification process for obtaining the desired structure with high purity ( > 99%). Finally, the procedure described here allows the obtaining of others PEGylation reagents. |
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Sociedade Brasileira de Química |
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2009 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000600012 |
work_keys_str_mv |
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1756387327774556160 |