Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.)
Anthracnose, caused by Colletotrichum gloeosporioides, produces brown lesions on guava fruits, causing severe losses on postharvest. In this study, the infection and colonization of guava fruits by C. gloeosporioides has been examined using scanning and transmission electron microscopy. Fruits at the physiologically mature stage were inoculated with a 10(5) conidia/mL spore suspension. Afterward, fruits were incubated at 25 °C in a wet chamber for periods of 6, 12, 24, 48, 96 and 120 h to allow examination of the infection and colonization process. Conidia germination and appressoria formation occurred six hours after inoculation (h.a.i). Penetration occurred directly via penetration pegs from appressoria, which penetrated the host cuticle 48 h.a.i. Notably, the appressoria did not produce an appressorial cone surrounding the penetration pore. Infection vesicles were found in epidermal cells 96 h.a.i. The same fungal structures were found in epidermal and parenchymal cells of the host 120 h.a.i. Colonization strategy of C. gloeosporioides on guava fruit was intracellular hemibiotrophic.
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Sociedade Brasileira de Fruticultura
2013
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oai:scielo:S0100-294520130002000392013-08-06Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.)Moraes,Sylvia Raquel GomesTanaka,Francisco André OsamaMassola Júnior,Nelson Sidnei colonization penetration pre-penetration ultrastructure Anthracnose, caused by Colletotrichum gloeosporioides, produces brown lesions on guava fruits, causing severe losses on postharvest. In this study, the infection and colonization of guava fruits by C. gloeosporioides has been examined using scanning and transmission electron microscopy. Fruits at the physiologically mature stage were inoculated with a 10(5) conidia/mL spore suspension. Afterward, fruits were incubated at 25 °C in a wet chamber for periods of 6, 12, 24, 48, 96 and 120 h to allow examination of the infection and colonization process. Conidia germination and appressoria formation occurred six hours after inoculation (h.a.i). Penetration occurred directly via penetration pegs from appressoria, which penetrated the host cuticle 48 h.a.i. Notably, the appressoria did not produce an appressorial cone surrounding the penetration pore. Infection vesicles were found in epidermal cells 96 h.a.i. The same fungal structures were found in epidermal and parenchymal cells of the host 120 h.a.i. Colonization strategy of C. gloeosporioides on guava fruit was intracellular hemibiotrophic.info:eu-repo/semantics/openAccessSociedade Brasileira de FruticulturaRevista Brasileira de Fruticultura v.35 n.2 20132013-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452013000200039en10.1590/S0100-29452013000200039 |
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Moraes,Sylvia Raquel Gomes Tanaka,Francisco André Osama Massola Júnior,Nelson Sidnei |
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Moraes,Sylvia Raquel Gomes Tanaka,Francisco André Osama Massola Júnior,Nelson Sidnei Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.) |
author_facet |
Moraes,Sylvia Raquel Gomes Tanaka,Francisco André Osama Massola Júnior,Nelson Sidnei |
author_sort |
Moraes,Sylvia Raquel Gomes |
title |
Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.) |
title_short |
Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.) |
title_full |
Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.) |
title_fullStr |
Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.) |
title_full_unstemmed |
Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.) |
title_sort |
histopathology of colletotrichum gloeosporioides on guava fruits (psidium guajava l.) |
description |
Anthracnose, caused by Colletotrichum gloeosporioides, produces brown lesions on guava fruits, causing severe losses on postharvest. In this study, the infection and colonization of guava fruits by C. gloeosporioides has been examined using scanning and transmission electron microscopy. Fruits at the physiologically mature stage were inoculated with a 10(5) conidia/mL spore suspension. Afterward, fruits were incubated at 25 °C in a wet chamber for periods of 6, 12, 24, 48, 96 and 120 h to allow examination of the infection and colonization process. Conidia germination and appressoria formation occurred six hours after inoculation (h.a.i). Penetration occurred directly via penetration pegs from appressoria, which penetrated the host cuticle 48 h.a.i. Notably, the appressoria did not produce an appressorial cone surrounding the penetration pore. Infection vesicles were found in epidermal cells 96 h.a.i. The same fungal structures were found in epidermal and parenchymal cells of the host 120 h.a.i. Colonization strategy of C. gloeosporioides on guava fruit was intracellular hemibiotrophic. |
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Sociedade Brasileira de Fruticultura |
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2013 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452013000200039 |
work_keys_str_mv |
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