Evaluation of Xylella fastidiosa genetic diversity by fAFPL markers

The first phytopathogenic bacterium with its DNA entirely sequenced is being detected and isolated from different host plants in several geographic regions. Although it causes diseases in cultures of economic importance, such as citrus, coffee, and grapevine little is known about the genetic relationships among different strains. Actually, all strains are grouped as a single species, Xylella fastidiosa, despite colonizing different hosts, developing symptoms, and different physiological and microbiological observed conditions. The existence of genetic diversity among X. fastidiosa strains was detected by different methodological techniques, since cultural to molecular methods. However, little is know about the phylogenetic relationships developed by Brazilian strains obtained from coffee and citrus plants. In order to evaluate it, fAFLP markers were used to verify genetic diversity and phylogenetic relationships developed by Brazilian and strange strains. fAFLP is an efficient technique, with high reproducibility that is currently used for bacterial typing and classification. The obtained results showed that Brazilian strains present genetic diversity and that the strains from this study were grouped distinctly according host and geographical origin like citrus-coffee, temecula-grapevine-mulberry and plum-elm.

Saved in:
Bibliographic Details
Main Authors: Kishi,Luciano Takeshi, Wickert,Ester, Lemos,Eliana Gertrudes de Macedo
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Fruticultura 2008
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452008000100037
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0100-29452008000100037
record_format ojs
spelling oai:scielo:S0100-294520080001000372008-05-06Evaluation of Xylella fastidiosa genetic diversity by fAFPL markersKishi,Luciano TakeshiWickert,EsterLemos,Eliana Gertrudes de Macedo fAFLP Xylella fastidiosa genetic diversity DNA fingerprinting The first phytopathogenic bacterium with its DNA entirely sequenced is being detected and isolated from different host plants in several geographic regions. Although it causes diseases in cultures of economic importance, such as citrus, coffee, and grapevine little is known about the genetic relationships among different strains. Actually, all strains are grouped as a single species, Xylella fastidiosa, despite colonizing different hosts, developing symptoms, and different physiological and microbiological observed conditions. The existence of genetic diversity among X. fastidiosa strains was detected by different methodological techniques, since cultural to molecular methods. However, little is know about the phylogenetic relationships developed by Brazilian strains obtained from coffee and citrus plants. In order to evaluate it, fAFLP markers were used to verify genetic diversity and phylogenetic relationships developed by Brazilian and strange strains. fAFLP is an efficient technique, with high reproducibility that is currently used for bacterial typing and classification. The obtained results showed that Brazilian strains present genetic diversity and that the strains from this study were grouped distinctly according host and geographical origin like citrus-coffee, temecula-grapevine-mulberry and plum-elm.info:eu-repo/semantics/openAccessSociedade Brasileira de FruticulturaRevista Brasileira de Fruticultura v.30 n.1 20082008-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452008000100037en10.1590/S0100-29452008000100037
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Kishi,Luciano Takeshi
Wickert,Ester
Lemos,Eliana Gertrudes de Macedo
spellingShingle Kishi,Luciano Takeshi
Wickert,Ester
Lemos,Eliana Gertrudes de Macedo
Evaluation of Xylella fastidiosa genetic diversity by fAFPL markers
author_facet Kishi,Luciano Takeshi
Wickert,Ester
Lemos,Eliana Gertrudes de Macedo
author_sort Kishi,Luciano Takeshi
title Evaluation of Xylella fastidiosa genetic diversity by fAFPL markers
title_short Evaluation of Xylella fastidiosa genetic diversity by fAFPL markers
title_full Evaluation of Xylella fastidiosa genetic diversity by fAFPL markers
title_fullStr Evaluation of Xylella fastidiosa genetic diversity by fAFPL markers
title_full_unstemmed Evaluation of Xylella fastidiosa genetic diversity by fAFPL markers
title_sort evaluation of xylella fastidiosa genetic diversity by fafpl markers
description The first phytopathogenic bacterium with its DNA entirely sequenced is being detected and isolated from different host plants in several geographic regions. Although it causes diseases in cultures of economic importance, such as citrus, coffee, and grapevine little is known about the genetic relationships among different strains. Actually, all strains are grouped as a single species, Xylella fastidiosa, despite colonizing different hosts, developing symptoms, and different physiological and microbiological observed conditions. The existence of genetic diversity among X. fastidiosa strains was detected by different methodological techniques, since cultural to molecular methods. However, little is know about the phylogenetic relationships developed by Brazilian strains obtained from coffee and citrus plants. In order to evaluate it, fAFLP markers were used to verify genetic diversity and phylogenetic relationships developed by Brazilian and strange strains. fAFLP is an efficient technique, with high reproducibility that is currently used for bacterial typing and classification. The obtained results showed that Brazilian strains present genetic diversity and that the strains from this study were grouped distinctly according host and geographical origin like citrus-coffee, temecula-grapevine-mulberry and plum-elm.
publisher Sociedade Brasileira de Fruticultura
publishDate 2008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452008000100037
work_keys_str_mv AT kishilucianotakeshi evaluationofxylellafastidiosageneticdiversitybyfafplmarkers
AT wickertester evaluationofxylellafastidiosageneticdiversitybyfafplmarkers
AT lemoselianagertrudesdemacedo evaluationofxylellafastidiosageneticdiversitybyfafplmarkers
_version_ 1756386277103501312