In vitro viability of genipap pollen grains in different culture media

In vitro viability of genipap pollen grains in different culture media

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Abstract The objective of this work was to evaluate the viability of genipap (Genipa americana) pollen grain at room temperature in different culture media. The experimental design was completely randomized in a 3x11 factorial arrangement (culture media x incubation times) with four replicates. The number of germinated pollen grains was analyzed at 24-hour intervals up to 288 hours after incubation at room temperature. The culture medium with 100 mg L-1 H3BO3, 80 g L-1 sucrose, and 1.0 g L-1 agar results in a higher to intermediate germination percentage, being the most suitable for studies on the in vitro viability of genipap pollen grains.

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Main Authors: Freire,Gilmara da Silva, Rocha,Letícia Bispo da, Machado,Caroline de Araújo, Silva,Ana Veruska Cruz da, Ledo,Ana da Silva
Format: Digital revista
Language:English
Published: Embrapa Secretaria de Pesquisa e Desenvolvimento 2022
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2022000102804
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spelling oai:scielo:S0100-204X20220001028042022-10-18In vitro viability of genipap pollen grains in different culture mediaFreire,Gilmara da SilvaRocha,Letícia Bispo daMachado,Caroline de AraújoSilva,Ana Veruska Cruz daLedo,Ana da Silva Genipa americana in vitro pollen germination plant breeding pollen tube Abstract The objective of this work was to evaluate the viability of genipap (Genipa americana) pollen grain at room temperature in different culture media. The experimental design was completely randomized in a 3x11 factorial arrangement (culture media x incubation times) with four replicates. The number of germinated pollen grains was analyzed at 24-hour intervals up to 288 hours after incubation at room temperature. The culture medium with 100 mg L-1 H3BO3, 80 g L-1 sucrose, and 1.0 g L-1 agar results in a higher to intermediate germination percentage, being the most suitable for studies on the in vitro viability of genipap pollen grains.info:eu-repo/semantics/openAccessEmbrapa Secretaria de Pesquisa e DesenvolvimentoPesquisa Agropecuária BrasileiraPesquisa Agropecuária Brasileira v.57 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2022000102804en10.1590/s1678-3921.pab2022.v57.03020
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Freire,Gilmara da Silva
Rocha,Letícia Bispo da
Machado,Caroline de Araújo
Silva,Ana Veruska Cruz da
Ledo,Ana da Silva
spellingShingle Freire,Gilmara da Silva
Rocha,Letícia Bispo da
Machado,Caroline de Araújo
Silva,Ana Veruska Cruz da
Ledo,Ana da Silva
In vitro viability of genipap pollen grains in different culture media
author_facet Freire,Gilmara da Silva
Rocha,Letícia Bispo da
Machado,Caroline de Araújo
Silva,Ana Veruska Cruz da
Ledo,Ana da Silva
author_sort Freire,Gilmara da Silva
title In vitro viability of genipap pollen grains in different culture media
title_short In vitro viability of genipap pollen grains in different culture media
title_full In vitro viability of genipap pollen grains in different culture media
title_fullStr In vitro viability of genipap pollen grains in different culture media
title_full_unstemmed In vitro viability of genipap pollen grains in different culture media
title_sort in vitro viability of genipap pollen grains in different culture media
description Abstract The objective of this work was to evaluate the viability of genipap (Genipa americana) pollen grain at room temperature in different culture media. The experimental design was completely randomized in a 3x11 factorial arrangement (culture media x incubation times) with four replicates. The number of germinated pollen grains was analyzed at 24-hour intervals up to 288 hours after incubation at room temperature. The culture medium with 100 mg L-1 H3BO3, 80 g L-1 sucrose, and 1.0 g L-1 agar results in a higher to intermediate germination percentage, being the most suitable for studies on the in vitro viability of genipap pollen grains.
publisher Embrapa Secretaria de Pesquisa e Desenvolvimento
publishDate 2022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2022000102804
work_keys_str_mv AT freiregilmaradasilva invitroviabilityofgenipappollengrainsindifferentculturemedia
AT rochaleticiabispoda invitroviabilityofgenipappollengrainsindifferentculturemedia
AT machadocarolinedearaujo invitroviabilityofgenipappollengrainsindifferentculturemedia
AT silvaanaveruskacruzda invitroviabilityofgenipappollengrainsindifferentculturemedia
AT ledoanadasilva invitroviabilityofgenipappollengrainsindifferentculturemedia
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