Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut

Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut

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Abstract: The objective of this work was to evaluate rewarming procedures and recovery media for Brazilian Green Dwarf coconut (Cocos nucifera) embryos cryopreserved by vitrification. The rewarming procedures evaluated were: T1, water bath at 38±2°C; and T2, unloading solution consisting of Y3 medium + 1.2 mol L-1 sucrose. The recovery media assessed were: M1, Y3 medium + 45 g L-1 sucrose + 1 mg L-1 gibberellic acid + 1 g L-1 activated charcoal + 2.2 g L-1 Gelrite; and M2, Y3 medium + 60 g L-1 sucrose + 2.2 g L-1 Gelrite. Rewarming procedures showed no significant effect, and the M1 media induced 72.5% regeneration.

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Main Authors: Lédo,Ana da Silva, Jenderek,Maria, Skogerboe,Dianne, Staats,Elise, Machado,Caroline de Araújo, Oliveira,Leila Albuquerque Resende de
Format: Digital revista
Language:English
Published: Embrapa Secretaria de Pesquisa e Desenvolvimento 2018
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000400514
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spelling oai:scielo:S0100-204X20180004005142018-06-25Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconutLédo,Ana da SilvaJenderek,MariaSkogerboe,DianneStaats,EliseMachado,Caroline de AraújoOliveira,Leila Albuquerque Resende de Cocos nucifera ex situ conservation PVS3 Abstract: The objective of this work was to evaluate rewarming procedures and recovery media for Brazilian Green Dwarf coconut (Cocos nucifera) embryos cryopreserved by vitrification. The rewarming procedures evaluated were: T1, water bath at 38±2°C; and T2, unloading solution consisting of Y3 medium + 1.2 mol L-1 sucrose. The recovery media assessed were: M1, Y3 medium + 45 g L-1 sucrose + 1 mg L-1 gibberellic acid + 1 g L-1 activated charcoal + 2.2 g L-1 Gelrite; and M2, Y3 medium + 60 g L-1 sucrose + 2.2 g L-1 Gelrite. Rewarming procedures showed no significant effect, and the M1 media induced 72.5% regeneration.info:eu-repo/semantics/openAccessEmbrapa Secretaria de Pesquisa e DesenvolvimentoPesquisa Agropecuária BrasileiraPesquisa Agropecuária Brasileira v.53 n.4 20182018-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000400514en10.1590/s0100-204x2018000400013
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Lédo,Ana da Silva
Jenderek,Maria
Skogerboe,Dianne
Staats,Elise
Machado,Caroline de Araújo
Oliveira,Leila Albuquerque Resende de
spellingShingle Lédo,Ana da Silva
Jenderek,Maria
Skogerboe,Dianne
Staats,Elise
Machado,Caroline de Araújo
Oliveira,Leila Albuquerque Resende de
Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut
author_facet Lédo,Ana da Silva
Jenderek,Maria
Skogerboe,Dianne
Staats,Elise
Machado,Caroline de Araújo
Oliveira,Leila Albuquerque Resende de
author_sort Lédo,Ana da Silva
title Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut
title_short Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut
title_full Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut
title_fullStr Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut
title_full_unstemmed Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut
title_sort cryopreservation of zygotic embryos of the brazilian green dwarf coconut
description Abstract: The objective of this work was to evaluate rewarming procedures and recovery media for Brazilian Green Dwarf coconut (Cocos nucifera) embryos cryopreserved by vitrification. The rewarming procedures evaluated were: T1, water bath at 38±2°C; and T2, unloading solution consisting of Y3 medium + 1.2 mol L-1 sucrose. The recovery media assessed were: M1, Y3 medium + 45 g L-1 sucrose + 1 mg L-1 gibberellic acid + 1 g L-1 activated charcoal + 2.2 g L-1 Gelrite; and M2, Y3 medium + 60 g L-1 sucrose + 2.2 g L-1 Gelrite. Rewarming procedures showed no significant effect, and the M1 media induced 72.5% regeneration.
publisher Embrapa Secretaria de Pesquisa e Desenvolvimento
publishDate 2018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000400514
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