Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPD
The objective of this work was to study the genetic variability of the grasshopper Rhammatocerus schistocercoides (Orthoptera: Acrididae) using RAPD analysis among individuals from three populations, one from Colombia and two from Brazil (Goiás and Mato Grosso States). Ninety scorable binary markers were obtained by fingerprinting with 11 oligonucleotide primers. Most of the polymorphism was attributed to 42 markers with variable frequency among the different populations. Although the existence of significant difference among populations (P<0.0001), most of the genetic variability was found within populations (87.7% of total variation). Pairwise distances between Colombian and Brazilian populations were 0.12 (P<0.0001) and 0.18 (P<0.0001) for Goiás and Mato Grosso, respectively. The pairwise distance between Goiás and Mato Grosso populations was 0.06 (P<0.0001). These data indicated that the phenotypic differences among populations are associated mainly with the geographical distances between the Brazilian and Colombian populations.
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Embrapa Secretaria de Pesquisa e Desenvolvimento
2002
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oai:scielo:S0100-204X20020011000202003-03-14Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPDSilva,João Batista Tavares daTigano,Myrian SilvanaMagalhães,Bonifácio PeixotoCordeiro,Célia Maria Torres Insecta genetic marker genetic variation genetic distance The objective of this work was to study the genetic variability of the grasshopper Rhammatocerus schistocercoides (Orthoptera: Acrididae) using RAPD analysis among individuals from three populations, one from Colombia and two from Brazil (Goiás and Mato Grosso States). Ninety scorable binary markers were obtained by fingerprinting with 11 oligonucleotide primers. Most of the polymorphism was attributed to 42 markers with variable frequency among the different populations. Although the existence of significant difference among populations (P<0.0001), most of the genetic variability was found within populations (87.7% of total variation). Pairwise distances between Colombian and Brazilian populations were 0.12 (P<0.0001) and 0.18 (P<0.0001) for Goiás and Mato Grosso, respectively. The pairwise distance between Goiás and Mato Grosso populations was 0.06 (P<0.0001). These data indicated that the phenotypic differences among populations are associated mainly with the geographical distances between the Brazilian and Colombian populations.info:eu-repo/semantics/openAccessEmbrapa Secretaria de Pesquisa e DesenvolvimentoPesquisa Agropecuária BrasileiraPesquisa Agropecuária Brasileira v.37 n.11 20022002-11-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002001100020en10.1590/S0100-204X2002001100020 |
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Silva,João Batista Tavares da Tigano,Myrian Silvana Magalhães,Bonifácio Peixoto Cordeiro,Célia Maria Torres |
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Silva,João Batista Tavares da Tigano,Myrian Silvana Magalhães,Bonifácio Peixoto Cordeiro,Célia Maria Torres Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPD |
author_facet |
Silva,João Batista Tavares da Tigano,Myrian Silvana Magalhães,Bonifácio Peixoto Cordeiro,Célia Maria Torres |
author_sort |
Silva,João Batista Tavares da |
title |
Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPD |
title_short |
Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPD |
title_full |
Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPD |
title_fullStr |
Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPD |
title_full_unstemmed |
Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPD |
title_sort |
polymorphism of the grasshopper rhammatocerus schistocercoides populations revealed by rapd |
description |
The objective of this work was to study the genetic variability of the grasshopper Rhammatocerus schistocercoides (Orthoptera: Acrididae) using RAPD analysis among individuals from three populations, one from Colombia and two from Brazil (Goiás and Mato Grosso States). Ninety scorable binary markers were obtained by fingerprinting with 11 oligonucleotide primers. Most of the polymorphism was attributed to 42 markers with variable frequency among the different populations. Although the existence of significant difference among populations (P<0.0001), most of the genetic variability was found within populations (87.7% of total variation). Pairwise distances between Colombian and Brazilian populations were 0.12 (P<0.0001) and 0.18 (P<0.0001) for Goiás and Mato Grosso, respectively. The pairwise distance between Goiás and Mato Grosso populations was 0.06 (P<0.0001). These data indicated that the phenotypic differences among populations are associated mainly with the geographical distances between the Brazilian and Colombian populations. |
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Embrapa Secretaria de Pesquisa e Desenvolvimento |
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2002 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002001100020 |
work_keys_str_mv |
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1756385616765911040 |