In vitro activity of Schinus terebinthifolius extract and fractions against Sporothrix brasiliensis
BACKGROUND Sporothrix brasiliensis is the causative agent of zoonotic cases of sporotrichosis in Brazil and is associated with atypical and severe presentations in cats, dogs, and humans. Sporotrichosis treatment is usually time- and cost-consuming, sometimes with poor response and host toxicity. Schinus terebinthifolius has proven efficacy against bacteria and fungi of clinical interest. OBJECTIVE To determine the in vitro activity of S. terebinthifolius against S. brasiliensis. METHODS Five S. brasiliensis isolates and three reference strains were subjected to a hydroethanol extract derived from the leaves of S. terebinthifolius and its fractions. The minimal inhibitory concentration (MIC) was determined using the broth microdilution method according to the M38-A2 CLSI guidelines. Also, the fungicidal/fungistatic activity of the extract and fractions was studied. FINDINGS The crude extract of S. terebinthifolius inhibited the growth of S. brasiliensis (MIC: 0.5-1.0 µg/mL), while the partitioned extracts dichloromethane, ethyl acetate, and butanol demonstrated growth inhibition at 8 µg/mL due to a fungistatic activity. MAIN CONCLUSIONS Due to its in vitro efficacy against S. brasiliensis and its known pharmacological safety, S. terebinthifolius is a candidate to be tested using in vivo models of sporotrichosis.
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Instituto Oswaldo Cruz, Ministério da Saúde
2022
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oai:scielo:S0074-027620220001011252022-09-26In vitro activity of Schinus terebinthifolius extract and fractions against Sporothrix brasiliensisAlmeida-Silva,FernandoBernardes-Engemann,Andrea ReisBérenger,Ana Luiza Rangelda Silva,Vagner PereiraFigueiredo,Maria RaquelFreitas,Dayvison Francis Saraiva Sporothrix brasiliensis sporotrichosis antifungal agents fungal drug sensitivity tests Schinus terebinthifolius BACKGROUND Sporothrix brasiliensis is the causative agent of zoonotic cases of sporotrichosis in Brazil and is associated with atypical and severe presentations in cats, dogs, and humans. Sporotrichosis treatment is usually time- and cost-consuming, sometimes with poor response and host toxicity. Schinus terebinthifolius has proven efficacy against bacteria and fungi of clinical interest. OBJECTIVE To determine the in vitro activity of S. terebinthifolius against S. brasiliensis. METHODS Five S. brasiliensis isolates and three reference strains were subjected to a hydroethanol extract derived from the leaves of S. terebinthifolius and its fractions. The minimal inhibitory concentration (MIC) was determined using the broth microdilution method according to the M38-A2 CLSI guidelines. Also, the fungicidal/fungistatic activity of the extract and fractions was studied. FINDINGS The crude extract of S. terebinthifolius inhibited the growth of S. brasiliensis (MIC: 0.5-1.0 µg/mL), while the partitioned extracts dichloromethane, ethyl acetate, and butanol demonstrated growth inhibition at 8 µg/mL due to a fungistatic activity. MAIN CONCLUSIONS Due to its in vitro efficacy against S. brasiliensis and its known pharmacological safety, S. terebinthifolius is a candidate to be tested using in vivo models of sporotrichosis.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.117 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762022000101125en10.1590/0074-02760220063 |
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author |
Almeida-Silva,Fernando Bernardes-Engemann,Andrea Reis Bérenger,Ana Luiza Rangel da Silva,Vagner Pereira Figueiredo,Maria Raquel Freitas,Dayvison Francis Saraiva |
spellingShingle |
Almeida-Silva,Fernando Bernardes-Engemann,Andrea Reis Bérenger,Ana Luiza Rangel da Silva,Vagner Pereira Figueiredo,Maria Raquel Freitas,Dayvison Francis Saraiva In vitro activity of Schinus terebinthifolius extract and fractions against Sporothrix brasiliensis |
author_facet |
Almeida-Silva,Fernando Bernardes-Engemann,Andrea Reis Bérenger,Ana Luiza Rangel da Silva,Vagner Pereira Figueiredo,Maria Raquel Freitas,Dayvison Francis Saraiva |
author_sort |
Almeida-Silva,Fernando |
title |
In vitro activity of Schinus terebinthifolius extract and fractions against Sporothrix brasiliensis |
title_short |
In vitro activity of Schinus terebinthifolius extract and fractions against Sporothrix brasiliensis |
title_full |
In vitro activity of Schinus terebinthifolius extract and fractions against Sporothrix brasiliensis |
title_fullStr |
In vitro activity of Schinus terebinthifolius extract and fractions against Sporothrix brasiliensis |
title_full_unstemmed |
In vitro activity of Schinus terebinthifolius extract and fractions against Sporothrix brasiliensis |
title_sort |
in vitro activity of schinus terebinthifolius extract and fractions against sporothrix brasiliensis |
description |
BACKGROUND Sporothrix brasiliensis is the causative agent of zoonotic cases of sporotrichosis in Brazil and is associated with atypical and severe presentations in cats, dogs, and humans. Sporotrichosis treatment is usually time- and cost-consuming, sometimes with poor response and host toxicity. Schinus terebinthifolius has proven efficacy against bacteria and fungi of clinical interest. OBJECTIVE To determine the in vitro activity of S. terebinthifolius against S. brasiliensis. METHODS Five S. brasiliensis isolates and three reference strains were subjected to a hydroethanol extract derived from the leaves of S. terebinthifolius and its fractions. The minimal inhibitory concentration (MIC) was determined using the broth microdilution method according to the M38-A2 CLSI guidelines. Also, the fungicidal/fungistatic activity of the extract and fractions was studied. FINDINGS The crude extract of S. terebinthifolius inhibited the growth of S. brasiliensis (MIC: 0.5-1.0 µg/mL), while the partitioned extracts dichloromethane, ethyl acetate, and butanol demonstrated growth inhibition at 8 µg/mL due to a fungistatic activity. MAIN CONCLUSIONS Due to its in vitro efficacy against S. brasiliensis and its known pharmacological safety, S. terebinthifolius is a candidate to be tested using in vivo models of sporotrichosis. |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2022 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762022000101125 |
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