Molecular and serological characterization of occult hepatitis B among blood donors in Maputo, Mozambique
BACKGROUND Occult hepatitis B virus (HBV) - characterized by the absence of detectable HBsAg in the presence of HBV DNA - represents a potential threat for blood safety. OBJECTIVES This study was conducted with the aim to investigate the serological and molecular characterization of occult HBV infection (OBI) among blood donors in Mozambique. METHODS 1,502 blood donors were tested for HBsAg. All HBsAg-negative individuals were tested for HBV DNA. Antibodies against HBV core, surface and HBe antigen (anti-HBc, anti-HBs, HBeAg) were measured in HBV DNA positive individuals. FINDINGS 1435 serum samples were HBsAg negative and 16 positive for HBV DNA, 14 confirmed to have OBI, corresponding to a frequency of 0.98%. Of the 14 OBI infections identified, 13/14 (92.8%) were positive for anti-HBc, 4/14 (28.5%) for anti-HBs, and no samples were reactive for HBeAg. Of the 14 OBI cases, nine samples (64.2%) were sequenced for the S/P region. Eight samples (88.9%) belonged to genotype A1 and one (11.1%) to genotype E. One escape mutation (T123A) associated with OBI and various amino acid substitutions for genotype A1 and E were observed. MAIN CONCLUSIONS Our results show the importance of using nucleic acid amplification test to detect occult hepatitis B infection in blood donors in Mozambique.
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Instituto Oswaldo Cruz, Ministério da Saúde
2020
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oai:scielo:S0074-027620200001003392020-09-18Molecular and serological characterization of occult hepatitis B among blood donors in Maputo, MozambiqueMabunda,NédioZicai,Ana FloraIsmael,NaliaVubil,AdolfoMello,FranciscoBlackard,Jason TLago,BarbaraDuarte,VanessaMoraes,MiltonLewis,LiaJani,Ilesh occult hepatitis B blood donors Mozambique BACKGROUND Occult hepatitis B virus (HBV) - characterized by the absence of detectable HBsAg in the presence of HBV DNA - represents a potential threat for blood safety. OBJECTIVES This study was conducted with the aim to investigate the serological and molecular characterization of occult HBV infection (OBI) among blood donors in Mozambique. METHODS 1,502 blood donors were tested for HBsAg. All HBsAg-negative individuals were tested for HBV DNA. Antibodies against HBV core, surface and HBe antigen (anti-HBc, anti-HBs, HBeAg) were measured in HBV DNA positive individuals. FINDINGS 1435 serum samples were HBsAg negative and 16 positive for HBV DNA, 14 confirmed to have OBI, corresponding to a frequency of 0.98%. Of the 14 OBI infections identified, 13/14 (92.8%) were positive for anti-HBc, 4/14 (28.5%) for anti-HBs, and no samples were reactive for HBeAg. Of the 14 OBI cases, nine samples (64.2%) were sequenced for the S/P region. Eight samples (88.9%) belonged to genotype A1 and one (11.1%) to genotype E. One escape mutation (T123A) associated with OBI and various amino acid substitutions for genotype A1 and E were observed. MAIN CONCLUSIONS Our results show the importance of using nucleic acid amplification test to detect occult hepatitis B infection in blood donors in Mozambique.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.115 20202020-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100339en10.1590/0074-02760200006 |
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Mabunda,Nédio Zicai,Ana Flora Ismael,Nalia Vubil,Adolfo Mello,Francisco Blackard,Jason T Lago,Barbara Duarte,Vanessa Moraes,Milton Lewis,Lia Jani,Ilesh |
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Mabunda,Nédio Zicai,Ana Flora Ismael,Nalia Vubil,Adolfo Mello,Francisco Blackard,Jason T Lago,Barbara Duarte,Vanessa Moraes,Milton Lewis,Lia Jani,Ilesh Molecular and serological characterization of occult hepatitis B among blood donors in Maputo, Mozambique |
author_facet |
Mabunda,Nédio Zicai,Ana Flora Ismael,Nalia Vubil,Adolfo Mello,Francisco Blackard,Jason T Lago,Barbara Duarte,Vanessa Moraes,Milton Lewis,Lia Jani,Ilesh |
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Mabunda,Nédio |
title |
Molecular and serological characterization of occult hepatitis B among blood donors in Maputo, Mozambique |
title_short |
Molecular and serological characterization of occult hepatitis B among blood donors in Maputo, Mozambique |
title_full |
Molecular and serological characterization of occult hepatitis B among blood donors in Maputo, Mozambique |
title_fullStr |
Molecular and serological characterization of occult hepatitis B among blood donors in Maputo, Mozambique |
title_full_unstemmed |
Molecular and serological characterization of occult hepatitis B among blood donors in Maputo, Mozambique |
title_sort |
molecular and serological characterization of occult hepatitis b among blood donors in maputo, mozambique |
description |
BACKGROUND Occult hepatitis B virus (HBV) - characterized by the absence of detectable HBsAg in the presence of HBV DNA - represents a potential threat for blood safety. OBJECTIVES This study was conducted with the aim to investigate the serological and molecular characterization of occult HBV infection (OBI) among blood donors in Mozambique. METHODS 1,502 blood donors were tested for HBsAg. All HBsAg-negative individuals were tested for HBV DNA. Antibodies against HBV core, surface and HBe antigen (anti-HBc, anti-HBs, HBeAg) were measured in HBV DNA positive individuals. FINDINGS 1435 serum samples were HBsAg negative and 16 positive for HBV DNA, 14 confirmed to have OBI, corresponding to a frequency of 0.98%. Of the 14 OBI infections identified, 13/14 (92.8%) were positive for anti-HBc, 4/14 (28.5%) for anti-HBs, and no samples were reactive for HBeAg. Of the 14 OBI cases, nine samples (64.2%) were sequenced for the S/P region. Eight samples (88.9%) belonged to genotype A1 and one (11.1%) to genotype E. One escape mutation (T123A) associated with OBI and various amino acid substitutions for genotype A1 and E were observed. MAIN CONCLUSIONS Our results show the importance of using nucleic acid amplification test to detect occult hepatitis B infection in blood donors in Mozambique. |
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Instituto Oswaldo Cruz, Ministério da Saúde |
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2020 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100339 |
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