First isolation of West Nile virus in Brazil
BACKGROUND Serological evidence of West Nile virus (WNV) infection has been reported in different regions of Brazil from equine and human hosts but the virus had never been isolated in the country. OBJECTIVES We sought to identify the viral etiology of equine encephalitis in Espírito Santo state. METHODS We performed viral culture in C6/36 cells, molecular detection of WNV genome, histopathology and immunohistochemistry from horse cerebral tissue. We also carried out sequencing, phylogenetic analysis and molecular clock. FINDINGS Histopathologic analysis from horse cerebral tissue showed injury related to encephalitis and WNV infection was confirmed by immunohistochemistry. The virus was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) from brain tissue and subsequently isolated in C6/36 cells. WNV full-length genome was sequenced showing the isolated strain belongs to lineage 1a. The molecular clock indicated that Brazilian WNV strain share the same common ancestor that were circulating in US during 2002-2005. MAIN CONCLUSIONS Here we report the first isolation of WNV in Brazil from a horse with neurologic disease, which was clustered into lineage 1a with others US WNV strains isolated in beginning of 2000’s decade.
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Instituto Oswaldo Cruz, Ministério da Saúde
2019
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oai:scielo:S0074-027620190001003052019-01-15First isolation of West Nile virus in BrazilMartins,Lívia CaricioSilva,Eliana Vieira Pinto daCasseb,Livia Medeiros NevesSilva,Sandro Patroca daCruz,Ana Cecília RibeiroPantoja,Jamilla Augusta de SousaMedeiros,Daniele Barbosa de AlmeidaMartins Filho,Arnaldo JorgeCruz,Ermelinda do Rosário Moutinho daAraújo,Marialva Tereza Ferreira deCardoso,Jedson FerreiraCunha,Marcos Antônio Correia Rodrigues daAlmada,Gilton LuizRomano,Alessandro Pecego MartinsSantos,Maria Guadalupe Dias PestanaRodrigues,Gilsa Aparecida PimentaChiang,Jannifer OliveiraQuaresma,Juarez Antonio SimõesCarvalho,Valéria LimaVasconcelos,Pedro Fernando da Costa West Nile virus horses Brazil BACKGROUND Serological evidence of West Nile virus (WNV) infection has been reported in different regions of Brazil from equine and human hosts but the virus had never been isolated in the country. OBJECTIVES We sought to identify the viral etiology of equine encephalitis in Espírito Santo state. METHODS We performed viral culture in C6/36 cells, molecular detection of WNV genome, histopathology and immunohistochemistry from horse cerebral tissue. We also carried out sequencing, phylogenetic analysis and molecular clock. FINDINGS Histopathologic analysis from horse cerebral tissue showed injury related to encephalitis and WNV infection was confirmed by immunohistochemistry. The virus was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) from brain tissue and subsequently isolated in C6/36 cells. WNV full-length genome was sequenced showing the isolated strain belongs to lineage 1a. The molecular clock indicated that Brazilian WNV strain share the same common ancestor that were circulating in US during 2002-2005. MAIN CONCLUSIONS Here we report the first isolation of WNV in Brazil from a horse with neurologic disease, which was clustered into lineage 1a with others US WNV strains isolated in beginning of 2000’s decade.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.114 20192019-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100305en10.1590/0074-02760180332 |
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Martins,Lívia Caricio Silva,Eliana Vieira Pinto da Casseb,Livia Medeiros Neves Silva,Sandro Patroca da Cruz,Ana Cecília Ribeiro Pantoja,Jamilla Augusta de Sousa Medeiros,Daniele Barbosa de Almeida Martins Filho,Arnaldo Jorge Cruz,Ermelinda do Rosário Moutinho da Araújo,Marialva Tereza Ferreira de Cardoso,Jedson Ferreira Cunha,Marcos Antônio Correia Rodrigues da Almada,Gilton Luiz Romano,Alessandro Pecego Martins Santos,Maria Guadalupe Dias Pestana Rodrigues,Gilsa Aparecida Pimenta Chiang,Jannifer Oliveira Quaresma,Juarez Antonio Simões Carvalho,Valéria Lima Vasconcelos,Pedro Fernando da Costa |
spellingShingle |
Martins,Lívia Caricio Silva,Eliana Vieira Pinto da Casseb,Livia Medeiros Neves Silva,Sandro Patroca da Cruz,Ana Cecília Ribeiro Pantoja,Jamilla Augusta de Sousa Medeiros,Daniele Barbosa de Almeida Martins Filho,Arnaldo Jorge Cruz,Ermelinda do Rosário Moutinho da Araújo,Marialva Tereza Ferreira de Cardoso,Jedson Ferreira Cunha,Marcos Antônio Correia Rodrigues da Almada,Gilton Luiz Romano,Alessandro Pecego Martins Santos,Maria Guadalupe Dias Pestana Rodrigues,Gilsa Aparecida Pimenta Chiang,Jannifer Oliveira Quaresma,Juarez Antonio Simões Carvalho,Valéria Lima Vasconcelos,Pedro Fernando da Costa First isolation of West Nile virus in Brazil |
author_facet |
Martins,Lívia Caricio Silva,Eliana Vieira Pinto da Casseb,Livia Medeiros Neves Silva,Sandro Patroca da Cruz,Ana Cecília Ribeiro Pantoja,Jamilla Augusta de Sousa Medeiros,Daniele Barbosa de Almeida Martins Filho,Arnaldo Jorge Cruz,Ermelinda do Rosário Moutinho da Araújo,Marialva Tereza Ferreira de Cardoso,Jedson Ferreira Cunha,Marcos Antônio Correia Rodrigues da Almada,Gilton Luiz Romano,Alessandro Pecego Martins Santos,Maria Guadalupe Dias Pestana Rodrigues,Gilsa Aparecida Pimenta Chiang,Jannifer Oliveira Quaresma,Juarez Antonio Simões Carvalho,Valéria Lima Vasconcelos,Pedro Fernando da Costa |
author_sort |
Martins,Lívia Caricio |
title |
First isolation of West Nile virus in Brazil |
title_short |
First isolation of West Nile virus in Brazil |
title_full |
First isolation of West Nile virus in Brazil |
title_fullStr |
First isolation of West Nile virus in Brazil |
title_full_unstemmed |
First isolation of West Nile virus in Brazil |
title_sort |
first isolation of west nile virus in brazil |
description |
BACKGROUND Serological evidence of West Nile virus (WNV) infection has been reported in different regions of Brazil from equine and human hosts but the virus had never been isolated in the country. OBJECTIVES We sought to identify the viral etiology of equine encephalitis in Espírito Santo state. METHODS We performed viral culture in C6/36 cells, molecular detection of WNV genome, histopathology and immunohistochemistry from horse cerebral tissue. We also carried out sequencing, phylogenetic analysis and molecular clock. FINDINGS Histopathologic analysis from horse cerebral tissue showed injury related to encephalitis and WNV infection was confirmed by immunohistochemistry. The virus was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) from brain tissue and subsequently isolated in C6/36 cells. WNV full-length genome was sequenced showing the isolated strain belongs to lineage 1a. The molecular clock indicated that Brazilian WNV strain share the same common ancestor that were circulating in US during 2002-2005. MAIN CONCLUSIONS Here we report the first isolation of WNV in Brazil from a horse with neurologic disease, which was clustered into lineage 1a with others US WNV strains isolated in beginning of 2000’s decade. |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100305 |
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