Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting
Abstract Brazil is one of the high burden countries for tuberculosis, and a rapid diagnosis is essential for effective control of the disease. In the present study, an in-house real-time polymerase chain reaction (PCR) assay targeting the mpt64 gene for identification of Mycobacterium tuberculosis complex isolates was evaluated under routine diagnosis conditions in a reference laboratory. From May 2011 to July 2012, 1,520 isolates of mycobacteria were prospectively submitted for phenotypic and/or PRA-hsp65 identification and to real-time PCR. The mpt64 real-time PCR showed 99.7% sensitivity and 96% specificity and detected 79.4% of the cases missed by phenotypic and PRA-hsp65 identification. The in-house real-time PCR assay showed high sensitivity and specificity and was successfully implemented in the routine diagnosis of tuberculosis in a reference laboratory from a high burden setting.
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Instituto Oswaldo Cruz, Ministério da Saúde
2016
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oai:scielo:S0074-027620160009005452016-09-15Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden settingGallo,Juliana FaildePinhata,Juliana Maira WatanabeChimara,EricaGonçalves,Maria GiseleFukasawa,Lucila OkuyamaOliveira,Rosangela Siqueira de molecular diagnostics MPT64 protein Mycobacterium tuberculosis real-time polymerase chain reaction tuberculosis Abstract Brazil is one of the high burden countries for tuberculosis, and a rapid diagnosis is essential for effective control of the disease. In the present study, an in-house real-time polymerase chain reaction (PCR) assay targeting the mpt64 gene for identification of Mycobacterium tuberculosis complex isolates was evaluated under routine diagnosis conditions in a reference laboratory. From May 2011 to July 2012, 1,520 isolates of mycobacteria were prospectively submitted for phenotypic and/or PRA-hsp65 identification and to real-time PCR. The mpt64 real-time PCR showed 99.7% sensitivity and 96% specificity and detected 79.4% of the cases missed by phenotypic and PRA-hsp65 identification. The in-house real-time PCR assay showed high sensitivity and specificity and was successfully implemented in the routine diagnosis of tuberculosis in a reference laboratory from a high burden setting.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.111 n.9 20162016-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000900545en10.1590/0074-02760160048 |
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Gallo,Juliana Failde Pinhata,Juliana Maira Watanabe Chimara,Erica Gonçalves,Maria Gisele Fukasawa,Lucila Okuyama Oliveira,Rosangela Siqueira de |
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Gallo,Juliana Failde Pinhata,Juliana Maira Watanabe Chimara,Erica Gonçalves,Maria Gisele Fukasawa,Lucila Okuyama Oliveira,Rosangela Siqueira de Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting |
author_facet |
Gallo,Juliana Failde Pinhata,Juliana Maira Watanabe Chimara,Erica Gonçalves,Maria Gisele Fukasawa,Lucila Okuyama Oliveira,Rosangela Siqueira de |
author_sort |
Gallo,Juliana Failde |
title |
Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting |
title_short |
Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting |
title_full |
Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting |
title_fullStr |
Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting |
title_full_unstemmed |
Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting |
title_sort |
performance of an in-house real-time polymerase chain reaction for identification of mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting |
description |
Abstract Brazil is one of the high burden countries for tuberculosis, and a rapid diagnosis is essential for effective control of the disease. In the present study, an in-house real-time polymerase chain reaction (PCR) assay targeting the mpt64 gene for identification of Mycobacterium tuberculosis complex isolates was evaluated under routine diagnosis conditions in a reference laboratory. From May 2011 to July 2012, 1,520 isolates of mycobacteria were prospectively submitted for phenotypic and/or PRA-hsp65 identification and to real-time PCR. The mpt64 real-time PCR showed 99.7% sensitivity and 96% specificity and detected 79.4% of the cases missed by phenotypic and PRA-hsp65 identification. The in-house real-time PCR assay showed high sensitivity and specificity and was successfully implemented in the routine diagnosis of tuberculosis in a reference laboratory from a high burden setting. |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2016 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000900545 |
work_keys_str_mv |
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