Mir-190b negatively contributes to the Trypanosoma cruzi- infected cell survival by repressing PTEN protein expression

Mir-190b negatively contributes to the Trypanosoma cruzi- infected cell survival by repressing PTEN protein expression

Chagas disease, which is caused by the intracellular protozoanTrypanosoma cruzi, is a serious health problem in Latin America. The heart is one of the major organs affected by this parasitic infection. The pathogenesis of tissue remodelling, particularly regarding cardiomyocyte behaviour after parasite infection, and the molecular mechanisms that occur immediately following parasite entry into host cells are not yet completely understood. Previous studies have reported that the establishment of parasitism is connected to the activation of the phosphatidylinositol-3 kinase (PI3K), which controls important steps in cellular metabolism by regulating the production of the second messenger phosphatidylinositol-3,4,5-trisphosphate. Particularly, the tumour suppressor PTEN is a negative regulator of PI3K signalling. However, mechanistic details of the modulatory activity of PTEN on Chagas disease have not been elucidated. To address this question, H9c2 cells were infected with T. cruzi Berenice 62 strain and the expression of a specific set of microRNAs (miRNAs) were investigated. Our cellular model demonstrated that miRNA-190b is correlated to the decrease of cellular viability rates by negatively modulating PTEN protein expression in T. cruzi-infected cells.

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Main Authors: Monteiro,Cíntia Júnia, Mota,Suianne Letícia Antunes, Diniz,Lívia de Figueiredo, Bahia,Maria Terezinha, Moraes,Karen CM
Format: Digital revista
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2015
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000800996
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spelling oai:scielo:S0074-027620150008009962015-12-16Mir-190b negatively contributes to the Trypanosoma cruzi- infected cell survival by repressing PTEN protein expressionMonteiro,Cíntia JúniaMota,Suianne Letícia AntunesDiniz,Lívia de FigueiredoBahia,Maria TerezinhaMoraes,Karen CM cardiac cellular model microRNA PTEN Trypanosoma cruzi Chagas disease, which is caused by the intracellular protozoanTrypanosoma cruzi, is a serious health problem in Latin America. The heart is one of the major organs affected by this parasitic infection. The pathogenesis of tissue remodelling, particularly regarding cardiomyocyte behaviour after parasite infection, and the molecular mechanisms that occur immediately following parasite entry into host cells are not yet completely understood. Previous studies have reported that the establishment of parasitism is connected to the activation of the phosphatidylinositol-3 kinase (PI3K), which controls important steps in cellular metabolism by regulating the production of the second messenger phosphatidylinositol-3,4,5-trisphosphate. Particularly, the tumour suppressor PTEN is a negative regulator of PI3K signalling. However, mechanistic details of the modulatory activity of PTEN on Chagas disease have not been elucidated. To address this question, H9c2 cells were infected with T. cruzi Berenice 62 strain and the expression of a specific set of microRNAs (miRNAs) were investigated. Our cellular model demonstrated that miRNA-190b is correlated to the decrease of cellular viability rates by negatively modulating PTEN protein expression in T. cruzi-infected cells.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.110 n.8 20152015-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000800996en10.1590/0074-02760150184
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Monteiro,Cíntia Júnia
Mota,Suianne Letícia Antunes
Diniz,Lívia de Figueiredo
Bahia,Maria Terezinha
Moraes,Karen CM
spellingShingle Monteiro,Cíntia Júnia
Mota,Suianne Letícia Antunes
Diniz,Lívia de Figueiredo
Bahia,Maria Terezinha
Moraes,Karen CM
Mir-190b negatively contributes to the Trypanosoma cruzi- infected cell survival by repressing PTEN protein expression
author_facet Monteiro,Cíntia Júnia
Mota,Suianne Letícia Antunes
Diniz,Lívia de Figueiredo
Bahia,Maria Terezinha
Moraes,Karen CM
author_sort Monteiro,Cíntia Júnia
title Mir-190b negatively contributes to the Trypanosoma cruzi- infected cell survival by repressing PTEN protein expression
title_short Mir-190b negatively contributes to the Trypanosoma cruzi- infected cell survival by repressing PTEN protein expression
title_full Mir-190b negatively contributes to the Trypanosoma cruzi- infected cell survival by repressing PTEN protein expression
title_fullStr Mir-190b negatively contributes to the Trypanosoma cruzi- infected cell survival by repressing PTEN protein expression
title_full_unstemmed Mir-190b negatively contributes to the Trypanosoma cruzi- infected cell survival by repressing PTEN protein expression
title_sort mir-190b negatively contributes to the trypanosoma cruzi- infected cell survival by repressing pten protein expression
description Chagas disease, which is caused by the intracellular protozoanTrypanosoma cruzi, is a serious health problem in Latin America. The heart is one of the major organs affected by this parasitic infection. The pathogenesis of tissue remodelling, particularly regarding cardiomyocyte behaviour after parasite infection, and the molecular mechanisms that occur immediately following parasite entry into host cells are not yet completely understood. Previous studies have reported that the establishment of parasitism is connected to the activation of the phosphatidylinositol-3 kinase (PI3K), which controls important steps in cellular metabolism by regulating the production of the second messenger phosphatidylinositol-3,4,5-trisphosphate. Particularly, the tumour suppressor PTEN is a negative regulator of PI3K signalling. However, mechanistic details of the modulatory activity of PTEN on Chagas disease have not been elucidated. To address this question, H9c2 cells were infected with T. cruzi Berenice 62 strain and the expression of a specific set of microRNAs (miRNAs) were investigated. Our cellular model demonstrated that miRNA-190b is correlated to the decrease of cellular viability rates by negatively modulating PTEN protein expression in T. cruzi-infected cells.
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000800996
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AT motasuianneleticiaantunes mir190bnegativelycontributestothetrypanosomacruziinfectedcellsurvivalbyrepressingptenproteinexpression
AT dinizliviadefigueiredo mir190bnegativelycontributestothetrypanosomacruziinfectedcellsurvivalbyrepressingptenproteinexpression
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