Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010
Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral structural proteins (VPs) and six nonstructural proteins (NSPs) are produced in RV-infected cells. NSP4 is a diarrhoea-inducing viral enterotoxin and NSP4 gene analysis revealed at least 15 (E1-E15) genotypes. This study analysed the NSP4 genetic diversity of HuRVA G2P[4] strains collected in the state of São Paulo (SP) from 1994 and 2006-2010 using reverse transcription-polymerase chain reaction, sequencing and phylogenetic analysis. Forty (97.6%) G2P[4] strains displayed genotype E2; one strain (2.4%) displayed genotype E1. These results are consistent with the proposed linkage between VP4/VP7 (G2P[4]) and the NSP4 (E2) genotype of HuRVA. NSP4 phylogenetic analysis showed distinct clusters, with grouping of most strains by their genotype and collection year, and most strains from SP were clustered together with strains from other Brazilian states. A deduced amino acid sequence alignment for E2 showed many variations in the C-terminal region, including the VP4-binding domain. Considering the ability of NSP4 to generate host immunity, monitoring NSP4 variations, along with those in the VP4 or VP7 protein, is important for evaluating the circulation and pathogenesis of RV. Finally, the presence of one G2P[4]E1 strain reinforces the idea that new genotype combinations emerge through reassortment and independent segregation.
Main Authors: | Bertol,Jéssica Wildgrube, Fregolente,Maria Clara Duarte, Caruzo,Thabata Alessandra Ramos, Silva,Márcio José da, Munford,Veridiana, Sáfadi,Marco Aurélio Palazzi, Rácz,Maria Lucia, Gatti,Maria Silvia Viccari |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2015
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600786 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Serological and molecular diversity of human rotavirus in São Paulo, Brazil
by: Munford,Veridiana, et al.
Published: (2007) -
Molecular characterization of G and P-types bovine rotavirus strains from Goiás, Brazil: high frequency of mixed P-type infections
by: Caruzo,Thabata Alessandra Ramos, et al.
Published: (2010) -
Characterization of mixed infections with different strains of bovine rotavirus in an outbreak of diarrhea in dairy herds in Goiás, Brazil
by: Brito,Wilia Marta Elsner Diederichsen de, et al.
Published: (2000) -
Association of rotavirus viroplasms with microtubules through NSP2 and NSP5
by: Cabral-Romero,Claudio, et al.
Published: (2006) -
G and P rotavirus genotypes in stool samples from children in Teresina, State of Piauí
by: Macedo,Carla Isabel, et al.
Published: (2007)