Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010

Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010

Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral structural proteins (VPs) and six nonstructural proteins (NSPs) are produced in RV-infected cells. NSP4 is a diarrhoea-inducing viral enterotoxin and NSP4 gene analysis revealed at least 15 (E1-E15) genotypes. This study analysed the NSP4 genetic diversity of HuRVA G2P[4] strains collected in the state of São Paulo (SP) from 1994 and 2006-2010 using reverse transcription-polymerase chain reaction, sequencing and phylogenetic analysis. Forty (97.6%) G2P[4] strains displayed genotype E2; one strain (2.4%) displayed genotype E1. These results are consistent with the proposed linkage between VP4/VP7 (G2P[4]) and the NSP4 (E2) genotype of HuRVA. NSP4 phylogenetic analysis showed distinct clusters, with grouping of most strains by their genotype and collection year, and most strains from SP were clustered together with strains from other Brazilian states. A deduced amino acid sequence alignment for E2 showed many variations in the C-terminal region, including the VP4-binding domain. Considering the ability of NSP4 to generate host immunity, monitoring NSP4 variations, along with those in the VP4 or VP7 protein, is important for evaluating the circulation and pathogenesis of RV. Finally, the presence of one G2P[4]E1 strain reinforces the idea that new genotype combinations emerge through reassortment and independent segregation.

Saved in:
Bibliographic Details
Main Authors: Bertol,Jéssica Wildgrube, Fregolente,Maria Clara Duarte, Caruzo,Thabata Alessandra Ramos, Silva,Márcio José da, Munford,Veridiana, Sáfadi,Marco Aurélio Palazzi, Rácz,Maria Lucia, Gatti,Maria Silvia Viccari
Format: Digital revista
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2015
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600786
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0074-02762015000600786
record_format ojs
spelling oai:scielo:S0074-027620150006007862015-11-05Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010Bertol,Jéssica WildgrubeFregolente,Maria Clara DuarteCaruzo,Thabata Alessandra RamosSilva,Márcio José daMunford,VeridianaSáfadi,Marco Aurélio PalazziRácz,Maria LuciaGatti,Maria Silvia Viccari human rotavirus NSP4 genotypes phylogenetic analysis VP4/VP7/NSP4 linkage Brazil Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral structural proteins (VPs) and six nonstructural proteins (NSPs) are produced in RV-infected cells. NSP4 is a diarrhoea-inducing viral enterotoxin and NSP4 gene analysis revealed at least 15 (E1-E15) genotypes. This study analysed the NSP4 genetic diversity of HuRVA G2P[4] strains collected in the state of São Paulo (SP) from 1994 and 2006-2010 using reverse transcription-polymerase chain reaction, sequencing and phylogenetic analysis. Forty (97.6%) G2P[4] strains displayed genotype E2; one strain (2.4%) displayed genotype E1. These results are consistent with the proposed linkage between VP4/VP7 (G2P[4]) and the NSP4 (E2) genotype of HuRVA. NSP4 phylogenetic analysis showed distinct clusters, with grouping of most strains by their genotype and collection year, and most strains from SP were clustered together with strains from other Brazilian states. A deduced amino acid sequence alignment for E2 showed many variations in the C-terminal region, including the VP4-binding domain. Considering the ability of NSP4 to generate host immunity, monitoring NSP4 variations, along with those in the VP4 or VP7 protein, is important for evaluating the circulation and pathogenesis of RV. Finally, the presence of one G2P[4]E1 strain reinforces the idea that new genotype combinations emerge through reassortment and independent segregation.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.110 n.6 20152015-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600786en10.1590/0074-02760150199
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Bertol,Jéssica Wildgrube
Fregolente,Maria Clara Duarte
Caruzo,Thabata Alessandra Ramos
Silva,Márcio José da
Munford,Veridiana
Sáfadi,Marco Aurélio Palazzi
Rácz,Maria Lucia
Gatti,Maria Silvia Viccari
spellingShingle Bertol,Jéssica Wildgrube
Fregolente,Maria Clara Duarte
Caruzo,Thabata Alessandra Ramos
Silva,Márcio José da
Munford,Veridiana
Sáfadi,Marco Aurélio Palazzi
Rácz,Maria Lucia
Gatti,Maria Silvia Viccari
Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010
author_facet Bertol,Jéssica Wildgrube
Fregolente,Maria Clara Duarte
Caruzo,Thabata Alessandra Ramos
Silva,Márcio José da
Munford,Veridiana
Sáfadi,Marco Aurélio Palazzi
Rácz,Maria Lucia
Gatti,Maria Silvia Viccari
author_sort Bertol,Jéssica Wildgrube
title Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010
title_short Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010
title_full Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010
title_fullStr Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010
title_full_unstemmed Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010
title_sort molecular characterisation of the nsp4 gene of group a human rotavirus g2p[4] strains circulating in são paulo, brazil, from 1994 and 2006 to 2010
description Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral structural proteins (VPs) and six nonstructural proteins (NSPs) are produced in RV-infected cells. NSP4 is a diarrhoea-inducing viral enterotoxin and NSP4 gene analysis revealed at least 15 (E1-E15) genotypes. This study analysed the NSP4 genetic diversity of HuRVA G2P[4] strains collected in the state of São Paulo (SP) from 1994 and 2006-2010 using reverse transcription-polymerase chain reaction, sequencing and phylogenetic analysis. Forty (97.6%) G2P[4] strains displayed genotype E2; one strain (2.4%) displayed genotype E1. These results are consistent with the proposed linkage between VP4/VP7 (G2P[4]) and the NSP4 (E2) genotype of HuRVA. NSP4 phylogenetic analysis showed distinct clusters, with grouping of most strains by their genotype and collection year, and most strains from SP were clustered together with strains from other Brazilian states. A deduced amino acid sequence alignment for E2 showed many variations in the C-terminal region, including the VP4-binding domain. Considering the ability of NSP4 to generate host immunity, monitoring NSP4 variations, along with those in the VP4 or VP7 protein, is important for evaluating the circulation and pathogenesis of RV. Finally, the presence of one G2P[4]E1 strain reinforces the idea that new genotype combinations emerge through reassortment and independent segregation.
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600786
work_keys_str_mv AT bertoljessicawildgrube molecularcharacterisationofthensp4geneofgroupahumanrotavirusg2p4strainscirculatinginsaopaulobrazilfrom1994and2006to2010
AT fregolentemariaclaraduarte molecularcharacterisationofthensp4geneofgroupahumanrotavirusg2p4strainscirculatinginsaopaulobrazilfrom1994and2006to2010
AT caruzothabataalessandraramos molecularcharacterisationofthensp4geneofgroupahumanrotavirusg2p4strainscirculatinginsaopaulobrazilfrom1994and2006to2010
AT silvamarciojoseda molecularcharacterisationofthensp4geneofgroupahumanrotavirusg2p4strainscirculatinginsaopaulobrazilfrom1994and2006to2010
AT munfordveridiana molecularcharacterisationofthensp4geneofgroupahumanrotavirusg2p4strainscirculatinginsaopaulobrazilfrom1994and2006to2010
AT safadimarcoaureliopalazzi molecularcharacterisationofthensp4geneofgroupahumanrotavirusg2p4strainscirculatinginsaopaulobrazilfrom1994and2006to2010
AT raczmarialucia molecularcharacterisationofthensp4geneofgroupahumanrotavirusg2p4strainscirculatinginsaopaulobrazilfrom1994and2006to2010
AT gattimariasilviaviccari molecularcharacterisationofthensp4geneofgroupahumanrotavirusg2p4strainscirculatinginsaopaulobrazilfrom1994and2006to2010
_version_ 1756383815954071552

Similar Items