Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes

Viral acute gastroenteritis (AG) is a significant cause of hospitalisation in children younger than five years. Group A rotavirus (RVA) is responsible for 30% of these cases. Following the introduction of RVA immunisation in Brazil in 2006, a decreased circulation of this virus has been observed. However, AG remains an important cause of hospitalisation of paediatric patients and only limited data are available regarding the role of other enteric viruses in these cases. We conducted a prospective study of paediatric patients hospitalised for AG. Stool samples were collected to investigate human adenovirus (HAdV), RVA, norovirus (NoV) and astrovirus (AstV). NoV typing was performed by nucleotide sequencing and phylogenetic analysis. From the 225 samples tested, 60 (26%) were positive for at least one viral agent. HAdV, NoV, RVA and AstV were detected in 16%, 8%, 6% and 0% of the samples, respectively. Mixed infections were found in nine patients: HAdV/RVA (5), HAdV/NoV (3) and HAdV/NoV/RVA (1). The frequency of fever and lymphocytosis was significantly higher in virus-infected patients. Phylogenetic analysis of NoV indicated that all of these viruses belonged to genotype GII.4. The significant frequency of these pathogens in patients with AG highlights the need to routinely implement laboratory investigations.

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Main Authors: Raboni,Sonia Maria, Damasio,Guilherme Augusto Costa, Ferreira,Carla EO, Pereira,Luciane A, Nogueira,Meri B, Vidal,Luine R, Cruz,Cristina R, Almeida,Sergio M
Format: Digital revista
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2014
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000400428
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spelling oai:scielo:S0074-027620140004004282014-07-24Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomesRaboni,Sonia MariaDamasio,Guilherme Augusto CostaFerreira,Carla EOPereira,Luciane ANogueira,Meri BVidal,Luine RCruz,Cristina RAlmeida,Sergio M gastroenteritis children rotavirus norovirus astrovirus human adenovirus Viral acute gastroenteritis (AG) is a significant cause of hospitalisation in children younger than five years. Group A rotavirus (RVA) is responsible for 30% of these cases. Following the introduction of RVA immunisation in Brazil in 2006, a decreased circulation of this virus has been observed. However, AG remains an important cause of hospitalisation of paediatric patients and only limited data are available regarding the role of other enteric viruses in these cases. We conducted a prospective study of paediatric patients hospitalised for AG. Stool samples were collected to investigate human adenovirus (HAdV), RVA, norovirus (NoV) and astrovirus (AstV). NoV typing was performed by nucleotide sequencing and phylogenetic analysis. From the 225 samples tested, 60 (26%) were positive for at least one viral agent. HAdV, NoV, RVA and AstV were detected in 16%, 8%, 6% and 0% of the samples, respectively. Mixed infections were found in nine patients: HAdV/RVA (5), HAdV/NoV (3) and HAdV/NoV/RVA (1). The frequency of fever and lymphocytosis was significantly higher in virus-infected patients. Phylogenetic analysis of NoV indicated that all of these viruses belonged to genotype GII.4. The significant frequency of these pathogens in patients with AG highlights the need to routinely implement laboratory investigations.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.109 n.4 20142014-07-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000400428en10.1590/0074-0276140066
institution SCIELO
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country Brasil
countrycode BR
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libraryname SciELO
language English
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author Raboni,Sonia Maria
Damasio,Guilherme Augusto Costa
Ferreira,Carla EO
Pereira,Luciane A
Nogueira,Meri B
Vidal,Luine R
Cruz,Cristina R
Almeida,Sergio M
spellingShingle Raboni,Sonia Maria
Damasio,Guilherme Augusto Costa
Ferreira,Carla EO
Pereira,Luciane A
Nogueira,Meri B
Vidal,Luine R
Cruz,Cristina R
Almeida,Sergio M
Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes
author_facet Raboni,Sonia Maria
Damasio,Guilherme Augusto Costa
Ferreira,Carla EO
Pereira,Luciane A
Nogueira,Meri B
Vidal,Luine R
Cruz,Cristina R
Almeida,Sergio M
author_sort Raboni,Sonia Maria
title Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes
title_short Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes
title_full Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes
title_fullStr Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes
title_full_unstemmed Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes
title_sort acute gastroenteritis and enteric viruses in hospitalised children in southern brazil: aetiology, seasonality and clinical outcomes
description Viral acute gastroenteritis (AG) is a significant cause of hospitalisation in children younger than five years. Group A rotavirus (RVA) is responsible for 30% of these cases. Following the introduction of RVA immunisation in Brazil in 2006, a decreased circulation of this virus has been observed. However, AG remains an important cause of hospitalisation of paediatric patients and only limited data are available regarding the role of other enteric viruses in these cases. We conducted a prospective study of paediatric patients hospitalised for AG. Stool samples were collected to investigate human adenovirus (HAdV), RVA, norovirus (NoV) and astrovirus (AstV). NoV typing was performed by nucleotide sequencing and phylogenetic analysis. From the 225 samples tested, 60 (26%) were positive for at least one viral agent. HAdV, NoV, RVA and AstV were detected in 16%, 8%, 6% and 0% of the samples, respectively. Mixed infections were found in nine patients: HAdV/RVA (5), HAdV/NoV (3) and HAdV/NoV/RVA (1). The frequency of fever and lymphocytosis was significantly higher in virus-infected patients. Phylogenetic analysis of NoV indicated that all of these viruses belonged to genotype GII.4. The significant frequency of these pathogens in patients with AG highlights the need to routinely implement laboratory investigations.
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000400428
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