The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient
The hypothesis that Helicobactermight be a risk factor for human liver diseases has arisen after the detection of Helicobacter DNA in hepatic tissue of patients with hepatobiliary diseases. Nevertheless, no explanation that justifies the presence of the bacterium in the human liver has been proposed. We evaluated the presence of Helicobacterin the liver of patients with hepatic diseases of different aetiologies. We prospectively evaluated 147 patients (106 with primary hepatic diseases and 41 with hepatic metastatic tumours) and 20 liver donors as controls. Helicobacter species were investigated in the liver by culture and specific 16S rDNA nested-polymerase chain reaction followed by sequencing. Serum and hepatic levels of representative cytokines of T regulatory cell, T helper (Th)1 and Th17 cell lineages were determined using enzyme linked immunosorbent assay. The data were evaluated using logistic models. Detection of Helicobacter pylori DNA in the liver was independently associated with hepatitis B virus/hepatitis C virus, pancreatic carcinoma and a cytokine pattern characterised by high interleukin (IL)-10, low/absent interferon-γ and decreased IL-17A concentrations (p < 10-3). The bacterial DNA was never detected in the liver of patients with alcoholic cirrhosis and autoimmune hepatitis that are associated with Th1/Th17 polarisation. H. pylori may be observed in the liver of patients with certain hepatic and pancreatic diseases, but this might depend on the patient cytokine profile.
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Instituto Oswaldo Cruz, Ministério da Saúde
2011
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oai:scielo:S0074-027620110006000162015-02-02The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patientSilva,Luciana DinizRocha,Andreia Maria CamargosRocha,Gifone AguiarMoura,Sílvia Beleza deRocha,Márcia Maria Negreiros PintoDani,RenatoMelo,Fabrício Freire deGuerra,Juliana BecattiniCastro,Lúcia Porto Fonseca deMendes,Guilherme SantiagoFerrari,Teresa Cristina de AbreuLima,Agnaldo SoaresQueiroz,Dulciene Maria Magalhães Helicobacter pylori liver diseases IL-10 IL-17 IFN-γ The hypothesis that Helicobactermight be a risk factor for human liver diseases has arisen after the detection of Helicobacter DNA in hepatic tissue of patients with hepatobiliary diseases. Nevertheless, no explanation that justifies the presence of the bacterium in the human liver has been proposed. We evaluated the presence of Helicobacterin the liver of patients with hepatic diseases of different aetiologies. We prospectively evaluated 147 patients (106 with primary hepatic diseases and 41 with hepatic metastatic tumours) and 20 liver donors as controls. Helicobacter species were investigated in the liver by culture and specific 16S rDNA nested-polymerase chain reaction followed by sequencing. Serum and hepatic levels of representative cytokines of T regulatory cell, T helper (Th)1 and Th17 cell lineages were determined using enzyme linked immunosorbent assay. The data were evaluated using logistic models. Detection of Helicobacter pylori DNA in the liver was independently associated with hepatitis B virus/hepatitis C virus, pancreatic carcinoma and a cytokine pattern characterised by high interleukin (IL)-10, low/absent interferon-γ and decreased IL-17A concentrations (p < 10-3). The bacterial DNA was never detected in the liver of patients with alcoholic cirrhosis and autoimmune hepatitis that are associated with Th1/Th17 polarisation. H. pylori may be observed in the liver of patients with certain hepatic and pancreatic diseases, but this might depend on the patient cytokine profile.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.106 n.6 20112011-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000600016en10.1590/S0074-02762011000600016 |
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Silva,Luciana Diniz Rocha,Andreia Maria Camargos Rocha,Gifone Aguiar Moura,Sílvia Beleza de Rocha,Márcia Maria Negreiros Pinto Dani,Renato Melo,Fabrício Freire de Guerra,Juliana Becattini Castro,Lúcia Porto Fonseca de Mendes,Guilherme Santiago Ferrari,Teresa Cristina de Abreu Lima,Agnaldo Soares Queiroz,Dulciene Maria Magalhães |
| spellingShingle |
Silva,Luciana Diniz Rocha,Andreia Maria Camargos Rocha,Gifone Aguiar Moura,Sílvia Beleza de Rocha,Márcia Maria Negreiros Pinto Dani,Renato Melo,Fabrício Freire de Guerra,Juliana Becattini Castro,Lúcia Porto Fonseca de Mendes,Guilherme Santiago Ferrari,Teresa Cristina de Abreu Lima,Agnaldo Soares Queiroz,Dulciene Maria Magalhães The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient |
| author_facet |
Silva,Luciana Diniz Rocha,Andreia Maria Camargos Rocha,Gifone Aguiar Moura,Sílvia Beleza de Rocha,Márcia Maria Negreiros Pinto Dani,Renato Melo,Fabrício Freire de Guerra,Juliana Becattini Castro,Lúcia Porto Fonseca de Mendes,Guilherme Santiago Ferrari,Teresa Cristina de Abreu Lima,Agnaldo Soares Queiroz,Dulciene Maria Magalhães |
| author_sort |
Silva,Luciana Diniz |
| title |
The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient |
| title_short |
The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient |
| title_full |
The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient |
| title_fullStr |
The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient |
| title_full_unstemmed |
The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient |
| title_sort |
presence of helicobacter pylori in the liver depends on the th1, th17 and treg cytokine profile of the patient |
| description |
The hypothesis that Helicobactermight be a risk factor for human liver diseases has arisen after the detection of Helicobacter DNA in hepatic tissue of patients with hepatobiliary diseases. Nevertheless, no explanation that justifies the presence of the bacterium in the human liver has been proposed. We evaluated the presence of Helicobacterin the liver of patients with hepatic diseases of different aetiologies. We prospectively evaluated 147 patients (106 with primary hepatic diseases and 41 with hepatic metastatic tumours) and 20 liver donors as controls. Helicobacter species were investigated in the liver by culture and specific 16S rDNA nested-polymerase chain reaction followed by sequencing. Serum and hepatic levels of representative cytokines of T regulatory cell, T helper (Th)1 and Th17 cell lineages were determined using enzyme linked immunosorbent assay. The data were evaluated using logistic models. Detection of Helicobacter pylori DNA in the liver was independently associated with hepatitis B virus/hepatitis C virus, pancreatic carcinoma and a cytokine pattern characterised by high interleukin (IL)-10, low/absent interferon-γ and decreased IL-17A concentrations (p < 10-3). The bacterial DNA was never detected in the liver of patients with alcoholic cirrhosis and autoimmune hepatitis that are associated with Th1/Th17 polarisation. H. pylori may be observed in the liver of patients with certain hepatic and pancreatic diseases, but this might depend on the patient cytokine profile. |
| publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
| publishDate |
2011 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000600016 |
| work_keys_str_mv |
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