Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil

Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil

We performed spoligotyping on 114 strains of the Mycobacterium tuberculosis (Mtb) complex that had been isolated from patients in Minas Gerais Health Units during 2004. A total of 82/114 (72%) clinical isolates were clustered and 32/114 (28%) were unique. Seven shared types containing nine strains were newly created. A total of nine patterns corresponded to unreported orphan strains, as evaluated against all of the strains recorded in the SITVIT2 proprietary database in the Institut Pasteur de la Guadeloupe. The major clades were composed of isolates that belong to the following genotypes: Latin-America and Mediterranean (63/114, 55.3%) (the ill-defined T superfamily) (12/114, 10.5%), Haarlem (8/114, 7%), X clade (6/114, 5.3%), S clade (3/114, 2.6%) and the East-African Indian and Manu types, each with 1/114 (0.9%) isolates. A considerable number of strains (n = 20, 17.5%) showed patterns that did not fall within any of the previously described major clades. We conclude the bulk of tuberculosis (TB) (92/114, 80.7%) in our location is recent evolutionary strains that belong to the principal genetic groups 2/3. Further studies on epidemiology of TB are required to understand Mtb biodiversity and TB transmission in this region.

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Main Authors: Miranda,Silvana Spíndola de, Carvalho,Wânia da Silva, Suffys,Philip Noel, Kritski,Afrânio Lineu, Oliveira,Martha, Zarate,Noemi, Zozio,Thierry, Rastogi,Nalin, Gicquel,Brigitte
Format: Digital revista
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2011
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000300003
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spelling oai:scielo:S0074-027620110003000032011-05-26Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, BrazilMiranda,Silvana Spíndola deCarvalho,Wânia da SilvaSuffys,Philip NoelKritski,Afrânio LineuOliveira,MarthaZarate,NoemiZozio,ThierryRastogi,NalinGicquel,Brigitte Mycobacterium tuberculosis spoligotyping Brazil Minas Gerais We performed spoligotyping on 114 strains of the Mycobacterium tuberculosis (Mtb) complex that had been isolated from patients in Minas Gerais Health Units during 2004. A total of 82/114 (72%) clinical isolates were clustered and 32/114 (28%) were unique. Seven shared types containing nine strains were newly created. A total of nine patterns corresponded to unreported orphan strains, as evaluated against all of the strains recorded in the SITVIT2 proprietary database in the Institut Pasteur de la Guadeloupe. The major clades were composed of isolates that belong to the following genotypes: Latin-America and Mediterranean (63/114, 55.3%) (the ill-defined T superfamily) (12/114, 10.5%), Haarlem (8/114, 7%), X clade (6/114, 5.3%), S clade (3/114, 2.6%) and the East-African Indian and Manu types, each with 1/114 (0.9%) isolates. A considerable number of strains (n = 20, 17.5%) showed patterns that did not fall within any of the previously described major clades. We conclude the bulk of tuberculosis (TB) (92/114, 80.7%) in our location is recent evolutionary strains that belong to the principal genetic groups 2/3. Further studies on epidemiology of TB are required to understand Mtb biodiversity and TB transmission in this region.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.106 n.3 20112011-05-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000300003en10.1590/S0074-02762011000300003
institution SCIELO
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country Brasil
countrycode BR
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region America del Sur
libraryname SciELO
language English
format Digital
author Miranda,Silvana Spíndola de
Carvalho,Wânia da Silva
Suffys,Philip Noel
Kritski,Afrânio Lineu
Oliveira,Martha
Zarate,Noemi
Zozio,Thierry
Rastogi,Nalin
Gicquel,Brigitte
spellingShingle Miranda,Silvana Spíndola de
Carvalho,Wânia da Silva
Suffys,Philip Noel
Kritski,Afrânio Lineu
Oliveira,Martha
Zarate,Noemi
Zozio,Thierry
Rastogi,Nalin
Gicquel,Brigitte
Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil
author_facet Miranda,Silvana Spíndola de
Carvalho,Wânia da Silva
Suffys,Philip Noel
Kritski,Afrânio Lineu
Oliveira,Martha
Zarate,Noemi
Zozio,Thierry
Rastogi,Nalin
Gicquel,Brigitte
author_sort Miranda,Silvana Spíndola de
title Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil
title_short Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil
title_full Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil
title_fullStr Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil
title_full_unstemmed Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil
title_sort spoligotyping of clinical mycobacterium tuberculosis isolates from the state of minas gerais, brazil
description We performed spoligotyping on 114 strains of the Mycobacterium tuberculosis (Mtb) complex that had been isolated from patients in Minas Gerais Health Units during 2004. A total of 82/114 (72%) clinical isolates were clustered and 32/114 (28%) were unique. Seven shared types containing nine strains were newly created. A total of nine patterns corresponded to unreported orphan strains, as evaluated against all of the strains recorded in the SITVIT2 proprietary database in the Institut Pasteur de la Guadeloupe. The major clades were composed of isolates that belong to the following genotypes: Latin-America and Mediterranean (63/114, 55.3%) (the ill-defined T superfamily) (12/114, 10.5%), Haarlem (8/114, 7%), X clade (6/114, 5.3%), S clade (3/114, 2.6%) and the East-African Indian and Manu types, each with 1/114 (0.9%) isolates. A considerable number of strains (n = 20, 17.5%) showed patterns that did not fall within any of the previously described major clades. We conclude the bulk of tuberculosis (TB) (92/114, 80.7%) in our location is recent evolutionary strains that belong to the principal genetic groups 2/3. Further studies on epidemiology of TB are required to understand Mtb biodiversity and TB transmission in this region.
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000300003
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