Evaluation of polymerase chain reaction for the detection of Paracoccidioides brasiliensis DNA on serum samples from patients with paracoccidioidomycosis
The aim of this study was to demonstrate the DNA of Paracoccidioides brasiliensis in human serum samples of patients with paracoccidioidomycosis using the polymerase chain reaction (PCR). The diagnosis of paracoccidioidomycosis (PCM) was defined by microscopic observation of the fungus on direct exam or histopathology, culture, and serological positivity. DNA from serum of 33 patients with PCM was extracted and submitted to nested-PCR using primers from the gp 43 gene. Only one sample was positive on nested-PCR. We conclude that the prevalence of fungemia in patients with different clinical forms of PCM is low, limiting the use of serum DNA detection as an alternative diagnostic tool.
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Digital revista |
| Language: | English |
| Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2006
|
| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000200015 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
oai:scielo:S0074-02762006000200015 |
|---|---|
| record_format |
ojs |
| spelling |
oai:scielo:S0074-027620060002000152006-06-26Evaluation of polymerase chain reaction for the detection of Paracoccidioides brasiliensis DNA on serum samples from patients with paracoccidioidomycosisCharbel,Cecília EugeniaLevi,José EduardoMartins,José Eduardo Costa paracoccidioidomycosis Paracoccidioides brasiliensis molecular diagnosis serum polymerase chain reaction The aim of this study was to demonstrate the DNA of Paracoccidioides brasiliensis in human serum samples of patients with paracoccidioidomycosis using the polymerase chain reaction (PCR). The diagnosis of paracoccidioidomycosis (PCM) was defined by microscopic observation of the fungus on direct exam or histopathology, culture, and serological positivity. DNA from serum of 33 patients with PCM was extracted and submitted to nested-PCR using primers from the gp 43 gene. Only one sample was positive on nested-PCR. We conclude that the prevalence of fungemia in patients with different clinical forms of PCM is low, limiting the use of serum DNA detection as an alternative diagnostic tool.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.101 n.2 20062006-03-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000200015en10.1590/S0074-02762006000200015 |
| institution |
SCIELO |
| collection |
OJS |
| country |
Brasil |
| countrycode |
BR |
| component |
Revista |
| access |
En linea |
| databasecode |
rev-scielo-br |
| tag |
revista |
| region |
America del Sur |
| libraryname |
SciELO |
| language |
English |
| format |
Digital |
| author |
Charbel,Cecília Eugenia Levi,José Eduardo Martins,José Eduardo Costa |
| spellingShingle |
Charbel,Cecília Eugenia Levi,José Eduardo Martins,José Eduardo Costa Evaluation of polymerase chain reaction for the detection of Paracoccidioides brasiliensis DNA on serum samples from patients with paracoccidioidomycosis |
| author_facet |
Charbel,Cecília Eugenia Levi,José Eduardo Martins,José Eduardo Costa |
| author_sort |
Charbel,Cecília Eugenia |
| title |
Evaluation of polymerase chain reaction for the detection of Paracoccidioides brasiliensis DNA on serum samples from patients with paracoccidioidomycosis |
| title_short |
Evaluation of polymerase chain reaction for the detection of Paracoccidioides brasiliensis DNA on serum samples from patients with paracoccidioidomycosis |
| title_full |
Evaluation of polymerase chain reaction for the detection of Paracoccidioides brasiliensis DNA on serum samples from patients with paracoccidioidomycosis |
| title_fullStr |
Evaluation of polymerase chain reaction for the detection of Paracoccidioides brasiliensis DNA on serum samples from patients with paracoccidioidomycosis |
| title_full_unstemmed |
Evaluation of polymerase chain reaction for the detection of Paracoccidioides brasiliensis DNA on serum samples from patients with paracoccidioidomycosis |
| title_sort |
evaluation of polymerase chain reaction for the detection of paracoccidioides brasiliensis dna on serum samples from patients with paracoccidioidomycosis |
| description |
The aim of this study was to demonstrate the DNA of Paracoccidioides brasiliensis in human serum samples of patients with paracoccidioidomycosis using the polymerase chain reaction (PCR). The diagnosis of paracoccidioidomycosis (PCM) was defined by microscopic observation of the fungus on direct exam or histopathology, culture, and serological positivity. DNA from serum of 33 patients with PCM was extracted and submitted to nested-PCR using primers from the gp 43 gene. Only one sample was positive on nested-PCR. We conclude that the prevalence of fungemia in patients with different clinical forms of PCM is low, limiting the use of serum DNA detection as an alternative diagnostic tool. |
| publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
| publishDate |
2006 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000200015 |
| work_keys_str_mv |
AT charbelceciliaeugenia evaluationofpolymerasechainreactionforthedetectionofparacoccidioidesbrasiliensisdnaonserumsamplesfrompatientswithparacoccidioidomycosis AT levijoseeduardo evaluationofpolymerasechainreactionforthedetectionofparacoccidioidesbrasiliensisdnaonserumsamplesfrompatientswithparacoccidioidomycosis AT martinsjoseeduardocosta evaluationofpolymerasechainreactionforthedetectionofparacoccidioidesbrasiliensisdnaonserumsamplesfrompatientswithparacoccidioidomycosis |
| _version_ |
1756383528024539136 |