Genetic structure of Triatoma venosa (Hemiptera: Reduviidae): molecular and morphometric evidence
Triatoma venosa presents a restricted geographical distribution in America and is considered as a secondary vector of Chagas disease in Colombia and Ecuador. A total of 120 adult insects were collected in domestic and peridomestic habitats in an endemic area of the department of Boyacá, Colombia, in order to determine their genetic structure through morphometric and molecular techniques. The head and wings of each specimen were used for the analyses of size, shape, and sexual dimorphism. A significant sexual dimorphism was found, although no differences in size among the studied groups were detected. Differences were found in the analyzed structures except for male heads. DNA was extracted from the legs in order to carry out the internal transcriber space-2 (ITS-2) amplification and the randon amplified polymorphic DNA (RAPD) analyses. Length polymorphisms were not detected in the ITS-2. Fst and Nm values were estimated (0.047 and 3.4, respectively). The high genetic flow found among the insects captured in the domicile and peridomiciliary environment does not permit a genetic differentiation, thus establishing the peridomicile as an important place for epidemiological surveillance.
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Instituto Oswaldo Cruz, Ministério da Saúde
2006
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oai:scielo:S0074-027620060001000082006-05-12Genetic structure of Triatoma venosa (Hemiptera: Reduviidae): molecular and morphometric evidenceVargas,ErikaEspitia,ClaudiaPatiño,CarlosPinto,NestorAguilera,GermánJaramillo,CarlosBargues,María DoloresGuhl,Felipe Triatoma venosa geometric morphometry internal transcriber space-2 random amplified polymorphic DNA vector control Triatoma venosa presents a restricted geographical distribution in America and is considered as a secondary vector of Chagas disease in Colombia and Ecuador. A total of 120 adult insects were collected in domestic and peridomestic habitats in an endemic area of the department of Boyacá, Colombia, in order to determine their genetic structure through morphometric and molecular techniques. The head and wings of each specimen were used for the analyses of size, shape, and sexual dimorphism. A significant sexual dimorphism was found, although no differences in size among the studied groups were detected. Differences were found in the analyzed structures except for male heads. DNA was extracted from the legs in order to carry out the internal transcriber space-2 (ITS-2) amplification and the randon amplified polymorphic DNA (RAPD) analyses. Length polymorphisms were not detected in the ITS-2. Fst and Nm values were estimated (0.047 and 3.4, respectively). The high genetic flow found among the insects captured in the domicile and peridomiciliary environment does not permit a genetic differentiation, thus establishing the peridomicile as an important place for epidemiological surveillance.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.101 n.1 20062006-02-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000100008en10.1590/S0074-02762006000100008 |
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Vargas,Erika Espitia,Claudia Patiño,Carlos Pinto,Nestor Aguilera,Germán Jaramillo,Carlos Bargues,María Dolores Guhl,Felipe |
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Vargas,Erika Espitia,Claudia Patiño,Carlos Pinto,Nestor Aguilera,Germán Jaramillo,Carlos Bargues,María Dolores Guhl,Felipe Genetic structure of Triatoma venosa (Hemiptera: Reduviidae): molecular and morphometric evidence |
author_facet |
Vargas,Erika Espitia,Claudia Patiño,Carlos Pinto,Nestor Aguilera,Germán Jaramillo,Carlos Bargues,María Dolores Guhl,Felipe |
author_sort |
Vargas,Erika |
title |
Genetic structure of Triatoma venosa (Hemiptera: Reduviidae): molecular and morphometric evidence |
title_short |
Genetic structure of Triatoma venosa (Hemiptera: Reduviidae): molecular and morphometric evidence |
title_full |
Genetic structure of Triatoma venosa (Hemiptera: Reduviidae): molecular and morphometric evidence |
title_fullStr |
Genetic structure of Triatoma venosa (Hemiptera: Reduviidae): molecular and morphometric evidence |
title_full_unstemmed |
Genetic structure of Triatoma venosa (Hemiptera: Reduviidae): molecular and morphometric evidence |
title_sort |
genetic structure of triatoma venosa (hemiptera: reduviidae): molecular and morphometric evidence |
description |
Triatoma venosa presents a restricted geographical distribution in America and is considered as a secondary vector of Chagas disease in Colombia and Ecuador. A total of 120 adult insects were collected in domestic and peridomestic habitats in an endemic area of the department of Boyacá, Colombia, in order to determine their genetic structure through morphometric and molecular techniques. The head and wings of each specimen were used for the analyses of size, shape, and sexual dimorphism. A significant sexual dimorphism was found, although no differences in size among the studied groups were detected. Differences were found in the analyzed structures except for male heads. DNA was extracted from the legs in order to carry out the internal transcriber space-2 (ITS-2) amplification and the randon amplified polymorphic DNA (RAPD) analyses. Length polymorphisms were not detected in the ITS-2. Fst and Nm values were estimated (0.047 and 3.4, respectively). The high genetic flow found among the insects captured in the domicile and peridomiciliary environment does not permit a genetic differentiation, thus establishing the peridomicile as an important place for epidemiological surveillance. |
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Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2006 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000100008 |
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