Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil

We report the frequency of the different diarrheagenic Escherichia coli (DEC) categories isolated from children with acute endemic diarrhea in Salvador, Bahia. The E. coli isolates were investigated by colony blot hibridization whit the following genes probes: eae, EAF, bfpA, Stx1, Stx2, ST-Ih, ST-Ip, LT-I, LT-II, INV, and EAEC, as virulence markers to distinguish typical and atypical EPEC, EHEC/STEC, ETEC, EIEC, and EAEC. Seven of the eight categories of DEC were detected. The most frequently isolated was atypical EPEC (10.1%) followed by ETEC (7.5%), and EAEC (4.2%). EHEC, STEC, EIEC, and typical EPEC were each detected once. The strains of ETEC, EAEC, and atypical EPEC belonged to a wide variety of serotypes. The serotypes of the others categories were O26:H11 (EHEC), O21:H21 (STEC), O142:H34 (typical EPEC), and O?H55 (EIEC). We also present the clinical manifestations and other pathogenic species observed in children with DEC. This is the first report of EHEC and STEC in Salvador, and one of the first in Brazil.

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Main Authors: Franzolin,Marcia Regina, Alves,Rosely Cabette Barbosa, Keller,Rogéria, Gomes,Tânia Aparecida Tardelli, Beutin,Lothar, Barreto,Mauricio Lima, Milroy,Craig, Strina,Agostino, Ribeiro,Hugo, Trabulsi,Luiz Rachid
Format: Digital revista
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2005
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000400004
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spelling oai:scielo:S0074-027620050004000042005-08-17Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, BrazilFranzolin,Marcia ReginaAlves,Rosely Cabette BarbosaKeller,RogériaGomes,Tânia Aparecida TardelliBeutin,LotharBarreto,Mauricio LimaMilroy,CraigStrina,AgostinoRibeiro,HugoTrabulsi,Luiz Rachid typical and atypical enteropathogenic Escherichia coli (EPEC) Shiga toxin producing E. coli (STEC) diarrhea virulence factors We report the frequency of the different diarrheagenic Escherichia coli (DEC) categories isolated from children with acute endemic diarrhea in Salvador, Bahia. The E. coli isolates were investigated by colony blot hibridization whit the following genes probes: eae, EAF, bfpA, Stx1, Stx2, ST-Ih, ST-Ip, LT-I, LT-II, INV, and EAEC, as virulence markers to distinguish typical and atypical EPEC, EHEC/STEC, ETEC, EIEC, and EAEC. Seven of the eight categories of DEC were detected. The most frequently isolated was atypical EPEC (10.1%) followed by ETEC (7.5%), and EAEC (4.2%). EHEC, STEC, EIEC, and typical EPEC were each detected once. The strains of ETEC, EAEC, and atypical EPEC belonged to a wide variety of serotypes. The serotypes of the others categories were O26:H11 (EHEC), O21:H21 (STEC), O142:H34 (typical EPEC), and O?H55 (EIEC). We also present the clinical manifestations and other pathogenic species observed in children with DEC. This is the first report of EHEC and STEC in Salvador, and one of the first in Brazil.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.100 n.4 20052005-07-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000400004en10.1590/S0074-02762005000400004
institution SCIELO
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country Brasil
countrycode BR
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access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
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author Franzolin,Marcia Regina
Alves,Rosely Cabette Barbosa
Keller,Rogéria
Gomes,Tânia Aparecida Tardelli
Beutin,Lothar
Barreto,Mauricio Lima
Milroy,Craig
Strina,Agostino
Ribeiro,Hugo
Trabulsi,Luiz Rachid
spellingShingle Franzolin,Marcia Regina
Alves,Rosely Cabette Barbosa
Keller,Rogéria
Gomes,Tânia Aparecida Tardelli
Beutin,Lothar
Barreto,Mauricio Lima
Milroy,Craig
Strina,Agostino
Ribeiro,Hugo
Trabulsi,Luiz Rachid
Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil
author_facet Franzolin,Marcia Regina
Alves,Rosely Cabette Barbosa
Keller,Rogéria
Gomes,Tânia Aparecida Tardelli
Beutin,Lothar
Barreto,Mauricio Lima
Milroy,Craig
Strina,Agostino
Ribeiro,Hugo
Trabulsi,Luiz Rachid
author_sort Franzolin,Marcia Regina
title Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil
title_short Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil
title_full Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil
title_fullStr Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil
title_full_unstemmed Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil
title_sort prevalence of diarrheagenic escherichia coli in children with diarrhea in salvador, bahia, brazil
description We report the frequency of the different diarrheagenic Escherichia coli (DEC) categories isolated from children with acute endemic diarrhea in Salvador, Bahia. The E. coli isolates were investigated by colony blot hibridization whit the following genes probes: eae, EAF, bfpA, Stx1, Stx2, ST-Ih, ST-Ip, LT-I, LT-II, INV, and EAEC, as virulence markers to distinguish typical and atypical EPEC, EHEC/STEC, ETEC, EIEC, and EAEC. Seven of the eight categories of DEC were detected. The most frequently isolated was atypical EPEC (10.1%) followed by ETEC (7.5%), and EAEC (4.2%). EHEC, STEC, EIEC, and typical EPEC were each detected once. The strains of ETEC, EAEC, and atypical EPEC belonged to a wide variety of serotypes. The serotypes of the others categories were O26:H11 (EHEC), O21:H21 (STEC), O142:H34 (typical EPEC), and O?H55 (EIEC). We also present the clinical manifestations and other pathogenic species observed in children with DEC. This is the first report of EHEC and STEC in Salvador, and one of the first in Brazil.
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000400004
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