Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil
We report the frequency of the different diarrheagenic Escherichia coli (DEC) categories isolated from children with acute endemic diarrhea in Salvador, Bahia. The E. coli isolates were investigated by colony blot hibridization whit the following genes probes: eae, EAF, bfpA, Stx1, Stx2, ST-Ih, ST-Ip, LT-I, LT-II, INV, and EAEC, as virulence markers to distinguish typical and atypical EPEC, EHEC/STEC, ETEC, EIEC, and EAEC. Seven of the eight categories of DEC were detected. The most frequently isolated was atypical EPEC (10.1%) followed by ETEC (7.5%), and EAEC (4.2%). EHEC, STEC, EIEC, and typical EPEC were each detected once. The strains of ETEC, EAEC, and atypical EPEC belonged to a wide variety of serotypes. The serotypes of the others categories were O26:H11 (EHEC), O21:H21 (STEC), O142:H34 (typical EPEC), and O?H55 (EIEC). We also present the clinical manifestations and other pathogenic species observed in children with DEC. This is the first report of EHEC and STEC in Salvador, and one of the first in Brazil.
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Instituto Oswaldo Cruz, Ministério da Saúde
2005
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oai:scielo:S0074-027620050004000042005-08-17Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, BrazilFranzolin,Marcia ReginaAlves,Rosely Cabette BarbosaKeller,RogériaGomes,Tânia Aparecida TardelliBeutin,LotharBarreto,Mauricio LimaMilroy,CraigStrina,AgostinoRibeiro,HugoTrabulsi,Luiz Rachid typical and atypical enteropathogenic Escherichia coli (EPEC) Shiga toxin producing E. coli (STEC) diarrhea virulence factors We report the frequency of the different diarrheagenic Escherichia coli (DEC) categories isolated from children with acute endemic diarrhea in Salvador, Bahia. The E. coli isolates were investigated by colony blot hibridization whit the following genes probes: eae, EAF, bfpA, Stx1, Stx2, ST-Ih, ST-Ip, LT-I, LT-II, INV, and EAEC, as virulence markers to distinguish typical and atypical EPEC, EHEC/STEC, ETEC, EIEC, and EAEC. Seven of the eight categories of DEC were detected. The most frequently isolated was atypical EPEC (10.1%) followed by ETEC (7.5%), and EAEC (4.2%). EHEC, STEC, EIEC, and typical EPEC were each detected once. The strains of ETEC, EAEC, and atypical EPEC belonged to a wide variety of serotypes. The serotypes of the others categories were O26:H11 (EHEC), O21:H21 (STEC), O142:H34 (typical EPEC), and O?H55 (EIEC). We also present the clinical manifestations and other pathogenic species observed in children with DEC. This is the first report of EHEC and STEC in Salvador, and one of the first in Brazil.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.100 n.4 20052005-07-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000400004en10.1590/S0074-02762005000400004 |
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Franzolin,Marcia Regina Alves,Rosely Cabette Barbosa Keller,Rogéria Gomes,Tânia Aparecida Tardelli Beutin,Lothar Barreto,Mauricio Lima Milroy,Craig Strina,Agostino Ribeiro,Hugo Trabulsi,Luiz Rachid |
spellingShingle |
Franzolin,Marcia Regina Alves,Rosely Cabette Barbosa Keller,Rogéria Gomes,Tânia Aparecida Tardelli Beutin,Lothar Barreto,Mauricio Lima Milroy,Craig Strina,Agostino Ribeiro,Hugo Trabulsi,Luiz Rachid Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil |
author_facet |
Franzolin,Marcia Regina Alves,Rosely Cabette Barbosa Keller,Rogéria Gomes,Tânia Aparecida Tardelli Beutin,Lothar Barreto,Mauricio Lima Milroy,Craig Strina,Agostino Ribeiro,Hugo Trabulsi,Luiz Rachid |
author_sort |
Franzolin,Marcia Regina |
title |
Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil |
title_short |
Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil |
title_full |
Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil |
title_fullStr |
Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil |
title_full_unstemmed |
Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil |
title_sort |
prevalence of diarrheagenic escherichia coli in children with diarrhea in salvador, bahia, brazil |
description |
We report the frequency of the different diarrheagenic Escherichia coli (DEC) categories isolated from children with acute endemic diarrhea in Salvador, Bahia. The E. coli isolates were investigated by colony blot hibridization whit the following genes probes: eae, EAF, bfpA, Stx1, Stx2, ST-Ih, ST-Ip, LT-I, LT-II, INV, and EAEC, as virulence markers to distinguish typical and atypical EPEC, EHEC/STEC, ETEC, EIEC, and EAEC. Seven of the eight categories of DEC were detected. The most frequently isolated was atypical EPEC (10.1%) followed by ETEC (7.5%), and EAEC (4.2%). EHEC, STEC, EIEC, and typical EPEC were each detected once. The strains of ETEC, EAEC, and atypical EPEC belonged to a wide variety of serotypes. The serotypes of the others categories were O26:H11 (EHEC), O21:H21 (STEC), O142:H34 (typical EPEC), and O?H55 (EIEC). We also present the clinical manifestations and other pathogenic species observed in children with DEC. This is the first report of EHEC and STEC in Salvador, and one of the first in Brazil. |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000400004 |
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