Ultrastructural study of the in vitro interaction between Biomphalaria glabrata hemocytes and Schistosoma mansoni miracidia

Ultrastructural study of the in vitro interaction between Biomphalaria glabrata hemocytes and Schistosoma mansoni miracidia

Biomphalaria glabrata and Schistosoma mansoni relationship was studied by light microscopy (LM) and freeze-fracture replica technique (FFR). We observed very thin cytoplasmic extensions of hemocytes in the LM, which then surround immobilize the miracidia. FFR images showed that the contact site between hemocytes cytoplasmic extensions and the external tegumentary coat involved only superficial layers of miracidia. Numerous vacuoles and filopodia were observed in the hemocyte cytoplasm, the latter binding with those from neighboring cells. In spite of the close interfilopodia contact, no cellular junctions were seen at these sites nor between filopodia-miracidia contact areas. The observed migration of hemocytes and their disposition in layers surrounding the miracidia in vitro correspond to previous studies.

Saved in:
Bibliographic Details
Main Authors: Araque,Wolfan, Barrios,Emilia E, Rodríguez,Pedro, Delgado,Víctor S, Finol,Héctor J
Format: Digital revista
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2003
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000700008
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0074-02762003000700008
record_format ojs
spelling oai:scielo:S0074-027620030007000082004-01-07Ultrastructural study of the in vitro interaction between Biomphalaria glabrata hemocytes and Schistosoma mansoni miracidiaAraque,WolfanBarrios,Emilia ERodríguez,PedroDelgado,Víctor SFinol,Héctor J Biomphalaria glabrata Schistosoma mansoni freeze-fracture replica Biomphalaria glabrata and Schistosoma mansoni relationship was studied by light microscopy (LM) and freeze-fracture replica technique (FFR). We observed very thin cytoplasmic extensions of hemocytes in the LM, which then surround immobilize the miracidia. FFR images showed that the contact site between hemocytes cytoplasmic extensions and the external tegumentary coat involved only superficial layers of miracidia. Numerous vacuoles and filopodia were observed in the hemocyte cytoplasm, the latter binding with those from neighboring cells. In spite of the close interfilopodia contact, no cellular junctions were seen at these sites nor between filopodia-miracidia contact areas. The observed migration of hemocytes and their disposition in layers surrounding the miracidia in vitro correspond to previous studies.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.98 n.7 20032003-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000700008en10.1590/S0074-02762003000700008
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Araque,Wolfan
Barrios,Emilia E
Rodríguez,Pedro
Delgado,Víctor S
Finol,Héctor J
spellingShingle Araque,Wolfan
Barrios,Emilia E
Rodríguez,Pedro
Delgado,Víctor S
Finol,Héctor J
Ultrastructural study of the in vitro interaction between Biomphalaria glabrata hemocytes and Schistosoma mansoni miracidia
author_facet Araque,Wolfan
Barrios,Emilia E
Rodríguez,Pedro
Delgado,Víctor S
Finol,Héctor J
author_sort Araque,Wolfan
title Ultrastructural study of the in vitro interaction between Biomphalaria glabrata hemocytes and Schistosoma mansoni miracidia
title_short Ultrastructural study of the in vitro interaction between Biomphalaria glabrata hemocytes and Schistosoma mansoni miracidia
title_full Ultrastructural study of the in vitro interaction between Biomphalaria glabrata hemocytes and Schistosoma mansoni miracidia
title_fullStr Ultrastructural study of the in vitro interaction between Biomphalaria glabrata hemocytes and Schistosoma mansoni miracidia
title_full_unstemmed Ultrastructural study of the in vitro interaction between Biomphalaria glabrata hemocytes and Schistosoma mansoni miracidia
title_sort ultrastructural study of the in vitro interaction between biomphalaria glabrata hemocytes and schistosoma mansoni miracidia
description Biomphalaria glabrata and Schistosoma mansoni relationship was studied by light microscopy (LM) and freeze-fracture replica technique (FFR). We observed very thin cytoplasmic extensions of hemocytes in the LM, which then surround immobilize the miracidia. FFR images showed that the contact site between hemocytes cytoplasmic extensions and the external tegumentary coat involved only superficial layers of miracidia. Numerous vacuoles and filopodia were observed in the hemocyte cytoplasm, the latter binding with those from neighboring cells. In spite of the close interfilopodia contact, no cellular junctions were seen at these sites nor between filopodia-miracidia contact areas. The observed migration of hemocytes and their disposition in layers surrounding the miracidia in vitro correspond to previous studies.
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000700008
work_keys_str_mv AT araquewolfan ultrastructuralstudyoftheinvitrointeractionbetweenbiomphalariaglabratahemocytesandschistosomamansonimiracidia
AT barriosemiliae ultrastructuralstudyoftheinvitrointeractionbetweenbiomphalariaglabratahemocytesandschistosomamansonimiracidia
AT rodriguezpedro ultrastructuralstudyoftheinvitrointeractionbetweenbiomphalariaglabratahemocytesandschistosomamansonimiracidia
AT delgadovictors ultrastructuralstudyoftheinvitrointeractionbetweenbiomphalariaglabratahemocytesandschistosomamansonimiracidia
AT finolhectorj ultrastructuralstudyoftheinvitrointeractionbetweenbiomphalariaglabratahemocytesandschistosomamansonimiracidia
_version_ 1756383462842957824

Similar Items