Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method

Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method

A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.

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Main Authors: Rubiano,Claudia C, Wasserman,Moises
Format: Digital revista
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2003
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500018
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spelling oai:scielo:S0074-027620030005000182003-09-04Detection of telomerase activity in Plasmodium falciparum using a nonradioactive methodRubiano,Claudia CWasserman,Moises Plasmodium malaria telomerase assay nonradioactive A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.98 n.5 20032003-07-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500018en10.1590/S0074-02762003000500018
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Rubiano,Claudia C
Wasserman,Moises
spellingShingle Rubiano,Claudia C
Wasserman,Moises
Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
author_facet Rubiano,Claudia C
Wasserman,Moises
author_sort Rubiano,Claudia C
title Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
title_short Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
title_full Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
title_fullStr Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
title_full_unstemmed Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
title_sort detection of telomerase activity in plasmodium falciparum using a nonradioactive method
description A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500018
work_keys_str_mv AT rubianoclaudiac detectionoftelomeraseactivityinplasmodiumfalciparumusinganonradioactivemethod
AT wassermanmoises detectionoftelomeraseactivityinplasmodiumfalciparumusinganonradioactivemethod
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