Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.
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Instituto Oswaldo Cruz, Ministério da Saúde
2003
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oai:scielo:S0074-027620030005000182003-09-04Detection of telomerase activity in Plasmodium falciparum using a nonradioactive methodRubiano,Claudia CWasserman,Moises Plasmodium malaria telomerase assay nonradioactive A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.98 n.5 20032003-07-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500018en10.1590/S0074-02762003000500018 |
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Rubiano,Claudia C Wasserman,Moises |
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Rubiano,Claudia C Wasserman,Moises Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method |
author_facet |
Rubiano,Claudia C Wasserman,Moises |
author_sort |
Rubiano,Claudia C |
title |
Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method |
title_short |
Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method |
title_full |
Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method |
title_fullStr |
Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method |
title_full_unstemmed |
Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method |
title_sort |
detection of telomerase activity in plasmodium falciparum using a nonradioactive method |
description |
A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells. |
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Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2003 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500018 |
work_keys_str_mv |
AT rubianoclaudiac detectionoftelomeraseactivityinplasmodiumfalciparumusinganonradioactivemethod AT wassermanmoises detectionoftelomeraseactivityinplasmodiumfalciparumusinganonradioactivemethod |
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1756383457751072768 |