Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain
Clone CL B5 of Trypanosoma cruzi is a beta-galactosidase expressing organism that was genetically transfected to be used for in vitro pharmacological screening. Biological parameters were determined, evaluating growth kinetics of epimastigotes, metacyclogenesis, infectivity to mammalian cell lines, parasitemia kinetics in mice and sensibility to nifurtimox and benznidazole. Differences in relation to other strains and CL parental strain were found, the most important being the incapability to produce death to mice in spite of the high inoculum used. However, it possesses the required features to be used for in vitro drug screening. Data obtained demonstrate that heterogeneity of T. cruzi appears even among clones of the same strain, and that these differences found do not prevent the use of clone CL B5 for the purpose that was engineered.
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Instituto Oswaldo Cruz, Ministério da Saúde
2002
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oai:scielo:S0074-027620020008000062003-01-20Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strainLe-Senne,AnaMuelas-Serrano,SusanaFernández-Portillo,CarlosEscario,José AntonioGómez-Barrio,Alicia Trypanosoma cruzi clone CL B5 biological characterization Clone CL B5 of Trypanosoma cruzi is a beta-galactosidase expressing organism that was genetically transfected to be used for in vitro pharmacological screening. Biological parameters were determined, evaluating growth kinetics of epimastigotes, metacyclogenesis, infectivity to mammalian cell lines, parasitemia kinetics in mice and sensibility to nifurtimox and benznidazole. Differences in relation to other strains and CL parental strain were found, the most important being the incapability to produce death to mice in spite of the high inoculum used. However, it possesses the required features to be used for in vitro drug screening. Data obtained demonstrate that heterogeneity of T. cruzi appears even among clones of the same strain, and that these differences found do not prevent the use of clone CL B5 for the purpose that was engineered.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.97 n.8 20022002-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800006en10.1590/S0074-02762002000800006 |
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Le-Senne,Ana Muelas-Serrano,Susana Fernández-Portillo,Carlos Escario,José Antonio Gómez-Barrio,Alicia |
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Le-Senne,Ana Muelas-Serrano,Susana Fernández-Portillo,Carlos Escario,José Antonio Gómez-Barrio,Alicia Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain |
author_facet |
Le-Senne,Ana Muelas-Serrano,Susana Fernández-Portillo,Carlos Escario,José Antonio Gómez-Barrio,Alicia |
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Le-Senne,Ana |
title |
Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain |
title_short |
Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain |
title_full |
Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain |
title_fullStr |
Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain |
title_full_unstemmed |
Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain |
title_sort |
biological characterization of a beta-galactosidase expressing clone of trypanosoma cruzi cl strain |
description |
Clone CL B5 of Trypanosoma cruzi is a beta-galactosidase expressing organism that was genetically transfected to be used for in vitro pharmacological screening. Biological parameters were determined, evaluating growth kinetics of epimastigotes, metacyclogenesis, infectivity to mammalian cell lines, parasitemia kinetics in mice and sensibility to nifurtimox and benznidazole. Differences in relation to other strains and CL parental strain were found, the most important being the incapability to produce death to mice in spite of the high inoculum used. However, it possesses the required features to be used for in vitro drug screening. Data obtained demonstrate that heterogeneity of T. cruzi appears even among clones of the same strain, and that these differences found do not prevent the use of clone CL B5 for the purpose that was engineered. |
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Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2002 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800006 |
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