Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites
Sera from 29 individuals residing in a malaria-endemic region of Colombia were evaluated by an inhibition assay for their capacity to retard the growth of Plasmodium falciparum in vitro. The inhibitory activity was found to be independent of antibody activity. Furthermore, the degree of inhibition of parasite development was variable, depending on the parasite isolate used for the assay and the season of malaria transmission. We selected sera with high inhibitory activity and carried out partial analytical characterization by anion exchange fast protein liquid chromatography (FPLC) to identify the chemical nature of the inhibitory factor(s). The results suggested that the in vitro inhibitory activity might result from the additive effect of different molecules. It appears that these molecules could be non-specifically induced by stimulation of the immune system, they seem to play a role in the immunity to malaria.
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Instituto Oswaldo Cruz, Ministério da Saúde
1994
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oai:scielo:S0074-027619940006000052009-06-15Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasitesPerlaza,Blanca L.Plata,Cecilia deZapata,ConstanzaRamírez,Jaime A.Herrera,Socrates malaria Plasmodium falciparum immunity crisis factor inhibition Sera from 29 individuals residing in a malaria-endemic region of Colombia were evaluated by an inhibition assay for their capacity to retard the growth of Plasmodium falciparum in vitro. The inhibitory activity was found to be independent of antibody activity. Furthermore, the degree of inhibition of parasite development was variable, depending on the parasite isolate used for the assay and the season of malaria transmission. We selected sera with high inhibitory activity and carried out partial analytical characterization by anion exchange fast protein liquid chromatography (FPLC) to identify the chemical nature of the inhibitory factor(s). The results suggested that the in vitro inhibitory activity might result from the additive effect of different molecules. It appears that these molecules could be non-specifically induced by stimulation of the immune system, they seem to play a role in the immunity to malaria.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.89 suppl.2 19941994-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000600005en10.1590/S0074-02761994000600005 |
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Perlaza,Blanca L. Plata,Cecilia de Zapata,Constanza Ramírez,Jaime A. Herrera,Socrates |
| spellingShingle |
Perlaza,Blanca L. Plata,Cecilia de Zapata,Constanza Ramírez,Jaime A. Herrera,Socrates Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites |
| author_facet |
Perlaza,Blanca L. Plata,Cecilia de Zapata,Constanza Ramírez,Jaime A. Herrera,Socrates |
| author_sort |
Perlaza,Blanca L. |
| title |
Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites |
| title_short |
Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites |
| title_full |
Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites |
| title_fullStr |
Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites |
| title_full_unstemmed |
Serum factors inhibitory for in vitro development of Plasmodium falciparum blood-stage parasites |
| title_sort |
serum factors inhibitory for in vitro development of plasmodium falciparum blood-stage parasites |
| description |
Sera from 29 individuals residing in a malaria-endemic region of Colombia were evaluated by an inhibition assay for their capacity to retard the growth of Plasmodium falciparum in vitro. The inhibitory activity was found to be independent of antibody activity. Furthermore, the degree of inhibition of parasite development was variable, depending on the parasite isolate used for the assay and the season of malaria transmission. We selected sera with high inhibitory activity and carried out partial analytical characterization by anion exchange fast protein liquid chromatography (FPLC) to identify the chemical nature of the inhibitory factor(s). The results suggested that the in vitro inhibitory activity might result from the additive effect of different molecules. It appears that these molecules could be non-specifically induced by stimulation of the immune system, they seem to play a role in the immunity to malaria. |
| publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
| publishDate |
1994 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000600005 |
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| _version_ |
1756383206932742144 |