A feasible method to extract DNA from the cambium of high-canopy trees: from harvest to assessment
ABSTRACT Many tropical trees have high canopies and their leaves are not accessible. Thus, the use of tissue from a more accessible organ (cambium) for DNA extraction may be an alternative for molecular studies. We adapted a feasible methodology for extracting genomic DNA from cambium tissue harvested in the field for the assessment with PCR. We tested three storage conditions (two buffers and a silica gel) and four periods of time after harvest. We used previously described protocols and tested them on three species that occur in Amazonian forests and other biomes: Anadenanthera peregrina var. peregrina, Cedrela fissilis, and Ceiba speciosa. Our protocol obtained suitable PCR-grade genomic DNA for DNA sequencing and microsatellite genotyping. We recommend the use of silica for long-term storage and the buffer with ascorbic acid for short-term storage.
Main Authors: | , , , , , |
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Format: | Digital revista |
Language: | English |
Published: |
Instituto Nacional de Pesquisas da Amazônia
2020
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Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400335 |
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