Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota)

ABSTRACT The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the cultures obtained. For both species, Potato Dextrose Agar (PDA) was the most promising of the tested culture media. The highest growth in agar culture ever recorded for this genus is reported (4.9 mm per week for G. lloydianum and 7.5 mm for G. subiculosum). In the PDA culture medium, spores germinated after 35-40 days of incubation and the isolation of monokaryotic cultures of the two species, as well as in vitro sexual crosses, were successfully performed.

Bibliographic Details

Main Authors:	SANTANA,Marcos Diones Ferreira, VARGAS-ISLA,Ruby, NOGUEIRA,Janaina da Costa, ACCIOLY,Thiago, SILVA,Bianca Denise Barbosa da, COUCEIRO,Sheyla Regina Marques, BASEIA,Iuri Goulart, ISHIKAWA,Noemia Kazue
Format:	Digital revista
Language:	English
Published:	Instituto Nacional de Pesquisas da Amazônia 2020
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000100061
Tags:	Add Tag No Tags, Be the first to tag this record!