Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota)
ABSTRACT The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the cultures obtained. For both species, Potato Dextrose Agar (PDA) was the most promising of the tested culture media. The highest growth in agar culture ever recorded for this genus is reported (4.9 mm per week for G. lloydianum and 7.5 mm for G. subiculosum). In the PDA culture medium, spores germinated after 35-40 days of incubation and the isolation of monokaryotic cultures of the two species, as well as in vitro sexual crosses, were successfully performed.
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Instituto Nacional de Pesquisas da Amazônia
2020
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oai:scielo:S0044-596720200001000612020-02-19Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota)SANTANA,Marcos Diones FerreiraVARGAS-ISLA,RubyNOGUEIRA,Janaina da CostaACCIOLY,ThiagoSILVA,Bianca Denise Barbosa daCOUCEIRO,Sheyla Regina MarquesBASEIA,Iuri GoulartISHIKAWA,Noemia Kazue earthstars gasteroid fungus Geastrum lloydianum Geastrum subiculosum mycelial growth ABSTRACT The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the cultures obtained. For both species, Potato Dextrose Agar (PDA) was the most promising of the tested culture media. The highest growth in agar culture ever recorded for this genus is reported (4.9 mm per week for G. lloydianum and 7.5 mm for G. subiculosum). In the PDA culture medium, spores germinated after 35-40 days of incubation and the isolation of monokaryotic cultures of the two species, as well as in vitro sexual crosses, were successfully performed.info:eu-repo/semantics/openAccessInstituto Nacional de Pesquisas da AmazôniaActa Amazonica v.50 n.1 20202020-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000100061en10.1590/1809-4392201901341 |
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SANTANA,Marcos Diones Ferreira VARGAS-ISLA,Ruby NOGUEIRA,Janaina da Costa ACCIOLY,Thiago SILVA,Bianca Denise Barbosa da COUCEIRO,Sheyla Regina Marques BASEIA,Iuri Goulart ISHIKAWA,Noemia Kazue |
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SANTANA,Marcos Diones Ferreira VARGAS-ISLA,Ruby NOGUEIRA,Janaina da Costa ACCIOLY,Thiago SILVA,Bianca Denise Barbosa da COUCEIRO,Sheyla Regina Marques BASEIA,Iuri Goulart ISHIKAWA,Noemia Kazue Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota) |
author_facet |
SANTANA,Marcos Diones Ferreira VARGAS-ISLA,Ruby NOGUEIRA,Janaina da Costa ACCIOLY,Thiago SILVA,Bianca Denise Barbosa da COUCEIRO,Sheyla Regina Marques BASEIA,Iuri Goulart ISHIKAWA,Noemia Kazue |
author_sort |
SANTANA,Marcos Diones Ferreira |
title |
Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota) |
title_short |
Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota) |
title_full |
Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota) |
title_fullStr |
Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota) |
title_full_unstemmed |
Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota) |
title_sort |
obtaining monokaryotic and dikaryotic mycelial cultures of two amazonian strains of geastrum (geastraceae, basidiomycota) |
description |
ABSTRACT The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the cultures obtained. For both species, Potato Dextrose Agar (PDA) was the most promising of the tested culture media. The highest growth in agar culture ever recorded for this genus is reported (4.9 mm per week for G. lloydianum and 7.5 mm for G. subiculosum). In the PDA culture medium, spores germinated after 35-40 days of incubation and the isolation of monokaryotic cultures of the two species, as well as in vitro sexual crosses, were successfully performed. |
publisher |
Instituto Nacional de Pesquisas da Amazônia |
publishDate |
2020 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000100061 |
work_keys_str_mv |
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