Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols

Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols

Abstract INTRODUCTION Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS After testing several different modifications, the adapted protocol with the best results in preliminary experiments was statistically evaluated in comparison with an automated robotic protocol. RESULTS Processing and testing of 119 nasopharyngeal samples ultimately yielded near-perfect agreement with the automated protocol (κ = 0.981 [95% confidence interval 0.943-1.000]). CONCLUSIONS The low cost and rapidity of the enhanced protocol makes it suitable for adoption in laboratories diagnosing COVID-19, especially those with high demand for examinations.

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Main Authors: Pessoa-e-Silva,Rômulo, Oliveira,Priscilla Stela Santana de, Gonçalves,Sayonara Maria Calado, Guarines,Klarissa Miranda, Carvalho,Lidiane Vasconcelos do Nascimento, Correia,Maria Andreza Bezerra, Rosa,Michelle Melgarejo da, Rêgo,Moacyr Jesus Barreto de Melo, Pitta,Maira Galdino da Rocha, Pereira,Michelly Cristiny
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2021
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822021000100657
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spelling oai:scielo:S0037-868220210001006572021-11-23Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocolsPessoa-e-Silva,RômuloOliveira,Priscilla Stela Santana deGonçalves,Sayonara Maria CaladoGuarines,Klarissa MirandaCarvalho,Lidiane Vasconcelos do NascimentoCorreia,Maria Andreza BezerraRosa,Michelle Melgarejo daRêgo,Moacyr Jesus Barreto de MeloPitta,Maira Galdino da RochaPereira,Michelly Cristiny COVID-19 SARS-CoV-2 Rapid extraction protocol RT-qPCR Abstract INTRODUCTION Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS After testing several different modifications, the adapted protocol with the best results in preliminary experiments was statistically evaluated in comparison with an automated robotic protocol. RESULTS Processing and testing of 119 nasopharyngeal samples ultimately yielded near-perfect agreement with the automated protocol (κ = 0.981 [95% confidence interval 0.943-1.000]). CONCLUSIONS The low cost and rapidity of the enhanced protocol makes it suitable for adoption in laboratories diagnosing COVID-19, especially those with high demand for examinations.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.54 20212021-01-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822021000100657en10.1590/0037-8682-0270-2021
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Pessoa-e-Silva,Rômulo
Oliveira,Priscilla Stela Santana de
Gonçalves,Sayonara Maria Calado
Guarines,Klarissa Miranda
Carvalho,Lidiane Vasconcelos do Nascimento
Correia,Maria Andreza Bezerra
Rosa,Michelle Melgarejo da
Rêgo,Moacyr Jesus Barreto de Melo
Pitta,Maira Galdino da Rocha
Pereira,Michelly Cristiny
spellingShingle Pessoa-e-Silva,Rômulo
Oliveira,Priscilla Stela Santana de
Gonçalves,Sayonara Maria Calado
Guarines,Klarissa Miranda
Carvalho,Lidiane Vasconcelos do Nascimento
Correia,Maria Andreza Bezerra
Rosa,Michelle Melgarejo da
Rêgo,Moacyr Jesus Barreto de Melo
Pitta,Maira Galdino da Rocha
Pereira,Michelly Cristiny
Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols
author_facet Pessoa-e-Silva,Rômulo
Oliveira,Priscilla Stela Santana de
Gonçalves,Sayonara Maria Calado
Guarines,Klarissa Miranda
Carvalho,Lidiane Vasconcelos do Nascimento
Correia,Maria Andreza Bezerra
Rosa,Michelle Melgarejo da
Rêgo,Moacyr Jesus Barreto de Melo
Pitta,Maira Galdino da Rocha
Pereira,Michelly Cristiny
author_sort Pessoa-e-Silva,Rômulo
title Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols
title_short Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols
title_full Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols
title_fullStr Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols
title_full_unstemmed Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols
title_sort enhanced rapid commercial dna extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: easy adaptation to current protocols
description Abstract INTRODUCTION Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS After testing several different modifications, the adapted protocol with the best results in preliminary experiments was statistically evaluated in comparison with an automated robotic protocol. RESULTS Processing and testing of 119 nasopharyngeal samples ultimately yielded near-perfect agreement with the automated protocol (κ = 0.981 [95% confidence interval 0.943-1.000]). CONCLUSIONS The low cost and rapidity of the enhanced protocol makes it suitable for adoption in laboratories diagnosing COVID-19, especially those with high demand for examinations.
publisher Sociedade Brasileira de Medicina Tropical - SBMT
publishDate 2021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822021000100657
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