Viral etiology of acute respiratory infections in children in Southern Iran
Abstract INTRODUCTION: Prevalence of influenza A virus (Flu-A), respiratory syncytial virus (RSV), and human metapneumovirus (hMPV) was assessed in children with acute respiratory infections (ARIs). METHODS: Nasopharyngeal aspirates and throat swabs were subjected to real-time polymerase chain reaction (PCR) to detect RSV and Flu-A and to conventional PCR to detect hMPV. RESULTS: Of the 156 children assessed, 93 (59.6%) carried at least one virus, with 35.9% positive for RSV, 14.1% for hMPV, and 9.6% for Flu-A. The prevalence of co-infections was 2.6%. CONCLUSIONS: The high detection rate may reflect increased sensitivity of real-time PCR compared to traditional PCR and viral culture.
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Sociedade Brasileira de Medicina Tropical - SBMT
2019
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oai:scielo:S0037-868220190001006822019-07-29Viral etiology of acute respiratory infections in children in Southern IranHalaji,MehrdadHashempour,TayebehMoayedi,JavadPouladfar,Gholam RezaKhansarinejad,BehzadKhashei,RezaMoattari,AfaghMusavi,ZahraGhassabi,FarzanehPirbonyeh,Neda Acute respiratory infections Respiratory syncytial vírus Influenza A vírus Human metapneumovirus Real-time polymerase chain reaction Abstract INTRODUCTION: Prevalence of influenza A virus (Flu-A), respiratory syncytial virus (RSV), and human metapneumovirus (hMPV) was assessed in children with acute respiratory infections (ARIs). METHODS: Nasopharyngeal aspirates and throat swabs were subjected to real-time polymerase chain reaction (PCR) to detect RSV and Flu-A and to conventional PCR to detect hMPV. RESULTS: Of the 156 children assessed, 93 (59.6%) carried at least one virus, with 35.9% positive for RSV, 14.1% for hMPV, and 9.6% for Flu-A. The prevalence of co-infections was 2.6%. CONCLUSIONS: The high detection rate may reflect increased sensitivity of real-time PCR compared to traditional PCR and viral culture.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.52 20192019-01-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100682en10.1590/0037-8682-0249-2018 |
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Halaji,Mehrdad Hashempour,Tayebeh Moayedi,Javad Pouladfar,Gholam Reza Khansarinejad,Behzad Khashei,Reza Moattari,Afagh Musavi,Zahra Ghassabi,Farzaneh Pirbonyeh,Neda |
| spellingShingle |
Halaji,Mehrdad Hashempour,Tayebeh Moayedi,Javad Pouladfar,Gholam Reza Khansarinejad,Behzad Khashei,Reza Moattari,Afagh Musavi,Zahra Ghassabi,Farzaneh Pirbonyeh,Neda Viral etiology of acute respiratory infections in children in Southern Iran |
| author_facet |
Halaji,Mehrdad Hashempour,Tayebeh Moayedi,Javad Pouladfar,Gholam Reza Khansarinejad,Behzad Khashei,Reza Moattari,Afagh Musavi,Zahra Ghassabi,Farzaneh Pirbonyeh,Neda |
| author_sort |
Halaji,Mehrdad |
| title |
Viral etiology of acute respiratory infections in children in Southern Iran |
| title_short |
Viral etiology of acute respiratory infections in children in Southern Iran |
| title_full |
Viral etiology of acute respiratory infections in children in Southern Iran |
| title_fullStr |
Viral etiology of acute respiratory infections in children in Southern Iran |
| title_full_unstemmed |
Viral etiology of acute respiratory infections in children in Southern Iran |
| title_sort |
viral etiology of acute respiratory infections in children in southern iran |
| description |
Abstract INTRODUCTION: Prevalence of influenza A virus (Flu-A), respiratory syncytial virus (RSV), and human metapneumovirus (hMPV) was assessed in children with acute respiratory infections (ARIs). METHODS: Nasopharyngeal aspirates and throat swabs were subjected to real-time polymerase chain reaction (PCR) to detect RSV and Flu-A and to conventional PCR to detect hMPV. RESULTS: Of the 156 children assessed, 93 (59.6%) carried at least one virus, with 35.9% positive for RSV, 14.1% for hMPV, and 9.6% for Flu-A. The prevalence of co-infections was 2.6%. CONCLUSIONS: The high detection rate may reflect increased sensitivity of real-time PCR compared to traditional PCR and viral culture. |
| publisher |
Sociedade Brasileira de Medicina Tropical - SBMT |
| publishDate |
2019 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100682 |
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