Use of Kisser glycerol gelatin to prepare microscope slides of Phlebotomine sandflies (Diptera: Psychodidae)
Abstract INTRODUCTION: Appropriate preservation of specimens is important for taxonomic identification. In sandfly research, various methods have been used for slide preparation; however, high cost, low commercial availability, and associated hazards make their use impossible in some studies. Therefore, the efficacy of Kisser glycerol gelatin for sandfly slide preparation was tested. METHODS: Kisser glycerol gelatin, as a substitute for Canada balsam and Berlese’s fluid, was used for mounting sandflies. RESULTS: Forty-two mounted specimens were created and maintained even after 14 months. CONCLUSIONS: Use of Kisser glycerol gelatin is simple and efficient for preparing microscope slides of sandflies.
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Sociedade Brasileira de Medicina Tropical - SBMT
2019
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oai:scielo:S0037-868220190001006122019-02-19Use of Kisser glycerol gelatin to prepare microscope slides of Phlebotomine sandflies (Diptera: Psychodidae)Holanda,Vanusa GonçalvesBarbosa,Diogo Brunno e SilvaPereira,Leudimar AiresSoares,Maria Regiane Araujo Sandfly Taxonomy Kisser glycerol gelatin Abstract INTRODUCTION: Appropriate preservation of specimens is important for taxonomic identification. In sandfly research, various methods have been used for slide preparation; however, high cost, low commercial availability, and associated hazards make their use impossible in some studies. Therefore, the efficacy of Kisser glycerol gelatin for sandfly slide preparation was tested. METHODS: Kisser glycerol gelatin, as a substitute for Canada balsam and Berlese’s fluid, was used for mounting sandflies. RESULTS: Forty-two mounted specimens were created and maintained even after 14 months. CONCLUSIONS: Use of Kisser glycerol gelatin is simple and efficient for preparing microscope slides of sandflies.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.52 20192019-01-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100612en10.1590/0037-8682-0072-2018 |
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Holanda,Vanusa Gonçalves Barbosa,Diogo Brunno e Silva Pereira,Leudimar Aires Soares,Maria Regiane Araujo |
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Holanda,Vanusa Gonçalves Barbosa,Diogo Brunno e Silva Pereira,Leudimar Aires Soares,Maria Regiane Araujo Use of Kisser glycerol gelatin to prepare microscope slides of Phlebotomine sandflies (Diptera: Psychodidae) |
| author_facet |
Holanda,Vanusa Gonçalves Barbosa,Diogo Brunno e Silva Pereira,Leudimar Aires Soares,Maria Regiane Araujo |
| author_sort |
Holanda,Vanusa Gonçalves |
| title |
Use of Kisser glycerol gelatin to prepare microscope slides of Phlebotomine sandflies (Diptera: Psychodidae) |
| title_short |
Use of Kisser glycerol gelatin to prepare microscope slides of Phlebotomine sandflies (Diptera: Psychodidae) |
| title_full |
Use of Kisser glycerol gelatin to prepare microscope slides of Phlebotomine sandflies (Diptera: Psychodidae) |
| title_fullStr |
Use of Kisser glycerol gelatin to prepare microscope slides of Phlebotomine sandflies (Diptera: Psychodidae) |
| title_full_unstemmed |
Use of Kisser glycerol gelatin to prepare microscope slides of Phlebotomine sandflies (Diptera: Psychodidae) |
| title_sort |
use of kisser glycerol gelatin to prepare microscope slides of phlebotomine sandflies (diptera: psychodidae) |
| description |
Abstract INTRODUCTION: Appropriate preservation of specimens is important for taxonomic identification. In sandfly research, various methods have been used for slide preparation; however, high cost, low commercial availability, and associated hazards make their use impossible in some studies. Therefore, the efficacy of Kisser glycerol gelatin for sandfly slide preparation was tested. METHODS: Kisser glycerol gelatin, as a substitute for Canada balsam and Berlese’s fluid, was used for mounting sandflies. RESULTS: Forty-two mounted specimens were created and maintained even after 14 months. CONCLUSIONS: Use of Kisser glycerol gelatin is simple and efficient for preparing microscope slides of sandflies. |
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Sociedade Brasileira de Medicina Tropical - SBMT |
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2019 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100612 |
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1756380650948001792 |