Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran

Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran

AbstractINTRODUCTIONThe aim of this study was to detect the prevalence of the extended-spectrum beta-lactamase (ESBL)-encoding CTX-M gene in Escherichia coliisolates.METHODS:Phenotypic screening of 376 E. coli isolates for ESBL was conducted using disk diffusion. ESBL-producing isolates were tested using PCR and specific primers. The blaCTX-M cluster was identified using the RFLP method, and its genotype was sequenced.RESULTS:From 202 ESBL-producing E. coli , 185 (91.5%) possessed CTX-M genes. CTX-M-1 subtypes were found in 98% of the isolates. The blaCTX-M gene was identical to CTX-M-15.CONCLUSIONS:A high prevalence of CTX-M-1-producing E. coli apparently exists in Shiraz, Iran.

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Main Authors: Zamani,Khosrow, Emami,Amir, Bazargani,Abdollah, Moattari,Afagh
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2015
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000400479
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spelling oai:scielo:S0037-868220150004004792015-08-25Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, IranZamani,KhosrowEmami,AmirBazargani,AbdollahMoattari,Afagh Escherichia coli CTX-M gene Iran. AbstractINTRODUCTIONThe aim of this study was to detect the prevalence of the extended-spectrum beta-lactamase (ESBL)-encoding CTX-M gene in Escherichia coliisolates.METHODS:Phenotypic screening of 376 E. coli isolates for ESBL was conducted using disk diffusion. ESBL-producing isolates were tested using PCR and specific primers. The blaCTX-M cluster was identified using the RFLP method, and its genotype was sequenced.RESULTS:From 202 ESBL-producing E. coli , 185 (91.5%) possessed CTX-M genes. CTX-M-1 subtypes were found in 98% of the isolates. The blaCTX-M gene was identical to CTX-M-15.CONCLUSIONS:A high prevalence of CTX-M-1-producing E. coli apparently exists in Shiraz, Iran.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.48 n.4 20152015-08-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000400479en10.1590/0037-8682-0315-2014
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Zamani,Khosrow
Emami,Amir
Bazargani,Abdollah
Moattari,Afagh
spellingShingle Zamani,Khosrow
Emami,Amir
Bazargani,Abdollah
Moattari,Afagh
Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran
author_facet Zamani,Khosrow
Emami,Amir
Bazargani,Abdollah
Moattari,Afagh
author_sort Zamani,Khosrow
title Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran
title_short Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran
title_full Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran
title_fullStr Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran
title_full_unstemmed Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran
title_sort phenotypic and molecular characterization of ctx-m extended-spectrum beta-lactamase-producing escherichia coli isolates in shiraz, iran
description AbstractINTRODUCTIONThe aim of this study was to detect the prevalence of the extended-spectrum beta-lactamase (ESBL)-encoding CTX-M gene in Escherichia coliisolates.METHODS:Phenotypic screening of 376 E. coli isolates for ESBL was conducted using disk diffusion. ESBL-producing isolates were tested using PCR and specific primers. The blaCTX-M cluster was identified using the RFLP method, and its genotype was sequenced.RESULTS:From 202 ESBL-producing E. coli , 185 (91.5%) possessed CTX-M genes. CTX-M-1 subtypes were found in 98% of the isolates. The blaCTX-M gene was identical to CTX-M-15.CONCLUSIONS:A high prevalence of CTX-M-1-producing E. coli apparently exists in Shiraz, Iran.
publisher Sociedade Brasileira de Medicina Tropical - SBMT
publishDate 2015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000400479
work_keys_str_mv AT zamanikhosrow phenotypicandmolecularcharacterizationofctxmextendedspectrumbetalactamaseproducingescherichiacoliisolatesinshiraziran
AT emamiamir phenotypicandmolecularcharacterizationofctxmextendedspectrumbetalactamaseproducingescherichiacoliisolatesinshiraziran
AT bazarganiabdollah phenotypicandmolecularcharacterizationofctxmextendedspectrumbetalactamaseproducingescherichiacoliisolatesinshiraziran
AT moattariafagh phenotypicandmolecularcharacterizationofctxmextendedspectrumbetalactamaseproducingescherichiacoliisolatesinshiraziran
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