Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti

Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti

IntroductionThe effects of piperonyl butoxide (PBO) on the toxicity of the organophosphate temephos (TE) and the role of esterases in the resistance of Aedes aegypti to this insecticide were evaluated.MethodsA. aegypti L4 larvae susceptible and resistant to TE were pre-treated with PBO solutions in acetone at concentrations of 0.125, 0.25, 0.5, 1, and 2% for 24h and subsequently exposed to a diagnostic concentration of 0.02mg/L aqueous TE solution. The esterase activity of the larvae extracts pre-treated with varying PBO concentrations and exposed to TE for three time periods was determined.ResultsAt concentrations of 0.25, 0.5, 1, and 2%, PBO showed a significant synergistic effect with TE toxicity. High levels of esterase activity were associated with the survival of A. aegypti L4 larvae exposed to TE only.ConclusionsThe results of the biochemical assays suggest that PBO has a significant inhibitory effect on the total esterase activity in A. aegypti larvae.

Saved in:
Bibliographic Details
Main Authors: Pereira,Boscolli Barbosa, Limongi,Jean Ezequiel, Campos Júnior,Edimar Olegário de, Luiz,Denis Prudencio, Kerr,Warwick Estevam
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2014
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500579
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0037-86822014000500579
record_format ojs
spelling oai:scielo:S0037-868220140005005792015-10-26Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegyptiPereira,Boscolli BarbosaLimongi,Jean EzequielCampos Júnior,Edimar Olegário deLuiz,Denis PrudencioKerr,Warwick Estevam Temephos Insecticide resistance Aedes aegypti Esterases IntroductionThe effects of piperonyl butoxide (PBO) on the toxicity of the organophosphate temephos (TE) and the role of esterases in the resistance of Aedes aegypti to this insecticide were evaluated.MethodsA. aegypti L4 larvae susceptible and resistant to TE were pre-treated with PBO solutions in acetone at concentrations of 0.125, 0.25, 0.5, 1, and 2% for 24h and subsequently exposed to a diagnostic concentration of 0.02mg/L aqueous TE solution. The esterase activity of the larvae extracts pre-treated with varying PBO concentrations and exposed to TE for three time periods was determined.ResultsAt concentrations of 0.25, 0.5, 1, and 2%, PBO showed a significant synergistic effect with TE toxicity. High levels of esterase activity were associated with the survival of A. aegypti L4 larvae exposed to TE only.ConclusionsThe results of the biochemical assays suggest that PBO has a significant inhibitory effect on the total esterase activity in A. aegypti larvae.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.47 n.5 20142014-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500579en10.1590/0037-8682-0195-2014
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Pereira,Boscolli Barbosa
Limongi,Jean Ezequiel
Campos Júnior,Edimar Olegário de
Luiz,Denis Prudencio
Kerr,Warwick Estevam
spellingShingle Pereira,Boscolli Barbosa
Limongi,Jean Ezequiel
Campos Júnior,Edimar Olegário de
Luiz,Denis Prudencio
Kerr,Warwick Estevam
Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti
author_facet Pereira,Boscolli Barbosa
Limongi,Jean Ezequiel
Campos Júnior,Edimar Olegário de
Luiz,Denis Prudencio
Kerr,Warwick Estevam
author_sort Pereira,Boscolli Barbosa
title Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti
title_short Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti
title_full Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti
title_fullStr Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti
title_full_unstemmed Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti
title_sort effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of aedes aegypti
description IntroductionThe effects of piperonyl butoxide (PBO) on the toxicity of the organophosphate temephos (TE) and the role of esterases in the resistance of Aedes aegypti to this insecticide were evaluated.MethodsA. aegypti L4 larvae susceptible and resistant to TE were pre-treated with PBO solutions in acetone at concentrations of 0.125, 0.25, 0.5, 1, and 2% for 24h and subsequently exposed to a diagnostic concentration of 0.02mg/L aqueous TE solution. The esterase activity of the larvae extracts pre-treated with varying PBO concentrations and exposed to TE for three time periods was determined.ResultsAt concentrations of 0.25, 0.5, 1, and 2%, PBO showed a significant synergistic effect with TE toxicity. High levels of esterase activity were associated with the survival of A. aegypti L4 larvae exposed to TE only.ConclusionsThe results of the biochemical assays suggest that PBO has a significant inhibitory effect on the total esterase activity in A. aegypti larvae.
publisher Sociedade Brasileira de Medicina Tropical - SBMT
publishDate 2014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500579
work_keys_str_mv AT pereiraboscollibarbosa effectsofpiperonylbutoxideonthetoxicityoftheorganophosphatetemephosandtheroleofesterasesintheinsecticideresistanceofaedesaegypti
AT limongijeanezequiel effectsofpiperonylbutoxideonthetoxicityoftheorganophosphatetemephosandtheroleofesterasesintheinsecticideresistanceofaedesaegypti
AT camposjunioredimarolegariode effectsofpiperonylbutoxideonthetoxicityoftheorganophosphatetemephosandtheroleofesterasesintheinsecticideresistanceofaedesaegypti
AT luizdenisprudencio effectsofpiperonylbutoxideonthetoxicityoftheorganophosphatetemephosandtheroleofesterasesintheinsecticideresistanceofaedesaegypti
AT kerrwarwickestevam effectsofpiperonylbutoxideonthetoxicityoftheorganophosphatetemephosandtheroleofesterasesintheinsecticideresistanceofaedesaegypti
_version_ 1756380538185187328

Similar Items