Cutaneous and visceral leishmaniasis co-infection in dogs from Rio de Janeiro, Brazil: evaluation by specific PCR and RFLP-PCR assays

Cutaneous and visceral leishmaniasis co-infection in dogs from Rio de Janeiro, Brazil: evaluation by specific PCR and RFLP-PCR assays

Introduction During a diagnostic evaluation of canine visceral leishmaniasis (VL), two of seventeen dogs were found to be co-infected by Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi. Methods Specific polymerase chain reaction (PCR) and restriction fragment length polymorphism-PCR (RFLP-PCR) assays were performed. Results PCR assays for Leishmania subgenus identification followed by RFLP-PCR analysis in biopsies from cutaneous lesions and the spleen confirmed the presence of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in those fragments. Conclusions This report reinforces the importance of using serological and molecular techniques in the epidemiological surveillance of canine populations in endemic areas in which both diseases are known to co-exist. In such cases, a reassessment of the control measures is required.

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Main Authors: Pires,Marize Quinhones, Madeira,Maria de Fátima, Bittencourt,Vânia Rita Elias Pinheiro, Pacheco,Raquel da Silva
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2014
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000200243
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spelling oai:scielo:S0037-868220140002002432014-05-15Cutaneous and visceral leishmaniasis co-infection in dogs from Rio de Janeiro, Brazil: evaluation by specific PCR and RFLP-PCR assaysPires,Marize QuinhonesMadeira,Maria de FátimaBittencourt,Vânia Rita Elias PinheiroPacheco,Raquel da Silva Visceral leishmaniasis Tegumentary leishmaniasis Restriction fragment length polymorphism-polymerase chain reaction Introduction During a diagnostic evaluation of canine visceral leishmaniasis (VL), two of seventeen dogs were found to be co-infected by Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi. Methods Specific polymerase chain reaction (PCR) and restriction fragment length polymorphism-PCR (RFLP-PCR) assays were performed. Results PCR assays for Leishmania subgenus identification followed by RFLP-PCR analysis in biopsies from cutaneous lesions and the spleen confirmed the presence of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in those fragments. Conclusions This report reinforces the importance of using serological and molecular techniques in the epidemiological surveillance of canine populations in endemic areas in which both diseases are known to co-exist. In such cases, a reassessment of the control measures is required. info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.47 n.2 20142014-04-01text/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000200243en10.1590/0037-8682-0007-2013
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Pires,Marize Quinhones
Madeira,Maria de Fátima
Bittencourt,Vânia Rita Elias Pinheiro
Pacheco,Raquel da Silva
spellingShingle Pires,Marize Quinhones
Madeira,Maria de Fátima
Bittencourt,Vânia Rita Elias Pinheiro
Pacheco,Raquel da Silva
Cutaneous and visceral leishmaniasis co-infection in dogs from Rio de Janeiro, Brazil: evaluation by specific PCR and RFLP-PCR assays
author_facet Pires,Marize Quinhones
Madeira,Maria de Fátima
Bittencourt,Vânia Rita Elias Pinheiro
Pacheco,Raquel da Silva
author_sort Pires,Marize Quinhones
title Cutaneous and visceral leishmaniasis co-infection in dogs from Rio de Janeiro, Brazil: evaluation by specific PCR and RFLP-PCR assays
title_short Cutaneous and visceral leishmaniasis co-infection in dogs from Rio de Janeiro, Brazil: evaluation by specific PCR and RFLP-PCR assays
title_full Cutaneous and visceral leishmaniasis co-infection in dogs from Rio de Janeiro, Brazil: evaluation by specific PCR and RFLP-PCR assays
title_fullStr Cutaneous and visceral leishmaniasis co-infection in dogs from Rio de Janeiro, Brazil: evaluation by specific PCR and RFLP-PCR assays
title_full_unstemmed Cutaneous and visceral leishmaniasis co-infection in dogs from Rio de Janeiro, Brazil: evaluation by specific PCR and RFLP-PCR assays
title_sort cutaneous and visceral leishmaniasis co-infection in dogs from rio de janeiro, brazil: evaluation by specific pcr and rflp-pcr assays
description Introduction During a diagnostic evaluation of canine visceral leishmaniasis (VL), two of seventeen dogs were found to be co-infected by Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi. Methods Specific polymerase chain reaction (PCR) and restriction fragment length polymorphism-PCR (RFLP-PCR) assays were performed. Results PCR assays for Leishmania subgenus identification followed by RFLP-PCR analysis in biopsies from cutaneous lesions and the spleen confirmed the presence of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in those fragments. Conclusions This report reinforces the importance of using serological and molecular techniques in the epidemiological surveillance of canine populations in endemic areas in which both diseases are known to co-exist. In such cases, a reassessment of the control measures is required.
publisher Sociedade Brasileira de Medicina Tropical - SBMT
publishDate 2014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000200243
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AT bittencourtvaniaritaeliaspinheiro cutaneousandvisceralleishmaniasiscoinfectionindogsfromriodejaneirobrazilevaluationbyspecificpcrandrflppcrassays
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