Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia

INTRODUCTION: During the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the Western Brazilian Amazon. METHODS: Molecular characterization of the G and P genotypes was performed using RT-PCR and electropherotyping analysis by polyacrylamide gel electrophoresis. RESULTS: A total of 59 samples were confirmed as group A rotavirus. A long electrophoretic profile was exhibited by the G1P[8], G3P[8], and G4P[8] genotypes. The G1P[8] genotype was found in greater proportion. The short electropherotype was exhibited only by G2 genotype strains. CONCLUSIONS: The proportion of the rotavirus genotypes observed was not different from that in other areas of Brazil. This study is the first genotyping of rotavirus in the Western Brazilian Amazon.

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Main Authors: Costa,Maria Sandra Moura, Nogueira,Paulo Afonso, Magalhães,Gleicienne Félix, Taquita,Paula, Mariúba,Luis André, Penatti,Marilene, Orlandi,Patrícia Puccinelli
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2012
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400021
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spelling oai:scielo:S0037-868220120004000212012-08-23Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian AmazoniaCosta,Maria Sandra MouraNogueira,Paulo AfonsoMagalhães,Gleicienne FélixTaquita,PaulaMariúba,Luis AndréPenatti,MarileneOrlandi,Patrícia Puccinelli Group A rotavirus Genotyping Brazilian Amazonia INTRODUCTION: During the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the Western Brazilian Amazon. METHODS: Molecular characterization of the G and P genotypes was performed using RT-PCR and electropherotyping analysis by polyacrylamide gel electrophoresis. RESULTS: A total of 59 samples were confirmed as group A rotavirus. A long electrophoretic profile was exhibited by the G1P[8], G3P[8], and G4P[8] genotypes. The G1P[8] genotype was found in greater proportion. The short electropherotype was exhibited only by G2 genotype strains. CONCLUSIONS: The proportion of the rotavirus genotypes observed was not different from that in other areas of Brazil. This study is the first genotyping of rotavirus in the Western Brazilian Amazon.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.45 n.4 20122012-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400021en10.1590/S0037-86822012000400021
institution SCIELO
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country Brasil
countrycode BR
component Revista
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databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Costa,Maria Sandra Moura
Nogueira,Paulo Afonso
Magalhães,Gleicienne Félix
Taquita,Paula
Mariúba,Luis André
Penatti,Marilene
Orlandi,Patrícia Puccinelli
spellingShingle Costa,Maria Sandra Moura
Nogueira,Paulo Afonso
Magalhães,Gleicienne Félix
Taquita,Paula
Mariúba,Luis André
Penatti,Marilene
Orlandi,Patrícia Puccinelli
Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia
author_facet Costa,Maria Sandra Moura
Nogueira,Paulo Afonso
Magalhães,Gleicienne Félix
Taquita,Paula
Mariúba,Luis André
Penatti,Marilene
Orlandi,Patrícia Puccinelli
author_sort Costa,Maria Sandra Moura
title Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia
title_short Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia
title_full Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia
title_fullStr Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia
title_full_unstemmed Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia
title_sort rotavirus genotyping in gastroenteritis cases of an infantile population from western brazilian amazonia
description INTRODUCTION: During the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the Western Brazilian Amazon. METHODS: Molecular characterization of the G and P genotypes was performed using RT-PCR and electropherotyping analysis by polyacrylamide gel electrophoresis. RESULTS: A total of 59 samples were confirmed as group A rotavirus. A long electrophoretic profile was exhibited by the G1P[8], G3P[8], and G4P[8] genotypes. The G1P[8] genotype was found in greater proportion. The short electropherotype was exhibited only by G2 genotype strains. CONCLUSIONS: The proportion of the rotavirus genotypes observed was not different from that in other areas of Brazil. This study is the first genotyping of rotavirus in the Western Brazilian Amazon.
publisher Sociedade Brasileira de Medicina Tropical - SBMT
publishDate 2012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400021
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