Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia
INTRODUCTION: During the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the Western Brazilian Amazon. METHODS: Molecular characterization of the G and P genotypes was performed using RT-PCR and electropherotyping analysis by polyacrylamide gel electrophoresis. RESULTS: A total of 59 samples were confirmed as group A rotavirus. A long electrophoretic profile was exhibited by the G1P[8], G3P[8], and G4P[8] genotypes. The G1P[8] genotype was found in greater proportion. The short electropherotype was exhibited only by G2 genotype strains. CONCLUSIONS: The proportion of the rotavirus genotypes observed was not different from that in other areas of Brazil. This study is the first genotyping of rotavirus in the Western Brazilian Amazon.
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Sociedade Brasileira de Medicina Tropical - SBMT
2012
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oai:scielo:S0037-868220120004000212012-08-23Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian AmazoniaCosta,Maria Sandra MouraNogueira,Paulo AfonsoMagalhães,Gleicienne FélixTaquita,PaulaMariúba,Luis AndréPenatti,MarileneOrlandi,Patrícia Puccinelli Group A rotavirus Genotyping Brazilian Amazonia INTRODUCTION: During the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the Western Brazilian Amazon. METHODS: Molecular characterization of the G and P genotypes was performed using RT-PCR and electropherotyping analysis by polyacrylamide gel electrophoresis. RESULTS: A total of 59 samples were confirmed as group A rotavirus. A long electrophoretic profile was exhibited by the G1P[8], G3P[8], and G4P[8] genotypes. The G1P[8] genotype was found in greater proportion. The short electropherotype was exhibited only by G2 genotype strains. CONCLUSIONS: The proportion of the rotavirus genotypes observed was not different from that in other areas of Brazil. This study is the first genotyping of rotavirus in the Western Brazilian Amazon.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.45 n.4 20122012-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400021en10.1590/S0037-86822012000400021 |
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Costa,Maria Sandra Moura Nogueira,Paulo Afonso Magalhães,Gleicienne Félix Taquita,Paula Mariúba,Luis André Penatti,Marilene Orlandi,Patrícia Puccinelli |
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Costa,Maria Sandra Moura Nogueira,Paulo Afonso Magalhães,Gleicienne Félix Taquita,Paula Mariúba,Luis André Penatti,Marilene Orlandi,Patrícia Puccinelli Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia |
author_facet |
Costa,Maria Sandra Moura Nogueira,Paulo Afonso Magalhães,Gleicienne Félix Taquita,Paula Mariúba,Luis André Penatti,Marilene Orlandi,Patrícia Puccinelli |
author_sort |
Costa,Maria Sandra Moura |
title |
Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia |
title_short |
Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia |
title_full |
Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia |
title_fullStr |
Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia |
title_full_unstemmed |
Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia |
title_sort |
rotavirus genotyping in gastroenteritis cases of an infantile population from western brazilian amazonia |
description |
INTRODUCTION: During the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the Western Brazilian Amazon. METHODS: Molecular characterization of the G and P genotypes was performed using RT-PCR and electropherotyping analysis by polyacrylamide gel electrophoresis. RESULTS: A total of 59 samples were confirmed as group A rotavirus. A long electrophoretic profile was exhibited by the G1P[8], G3P[8], and G4P[8] genotypes. The G1P[8] genotype was found in greater proportion. The short electropherotype was exhibited only by G2 genotype strains. CONCLUSIONS: The proportion of the rotavirus genotypes observed was not different from that in other areas of Brazil. This study is the first genotyping of rotavirus in the Western Brazilian Amazon. |
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Sociedade Brasileira de Medicina Tropical - SBMT |
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2012 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400021 |
work_keys_str_mv |
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