Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors
INTRODUCTION: HTLV-1/2 screening among blood donors commonly utilizes an enzyme-linked immunosorbent assay (EIA), followed by a confirmatory method such as Western blot (WB) if the EIA is positive. However, this algorithm yields a high rate of inconclusive results, and is expensive. METHODS: Two qualitative real-time PCR assays were developed to detect HTLV-1 and 2, and a total of 318 samples were tested (152 blood donors, 108 asymptomatic carriers, 26 HAM/TSP patients and 30 seronegative individuals). RESULTS: The sensitivity and specificity of PCR in comparison with WB results were 99.4% and 98.5%, respectively. PCR tests were more efficient for identifying the virus type, detecting HTLV-2 infection and defining inconclusive cases. CONCLUSIONS: Because real-time PCR is sensitive and practical and costs much less than WB, this technique can be used as a confirmatory test for HTLV in blood banks, as a replacement for WB.
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Sociedade Brasileira de Medicina Tropical - SBMT
2010
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oai:scielo:S0037-868220100002000012010-04-20Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donorsAndrade,Rafaela GomesRibeiro,Maísa AparecidaNamen-Lopes,Maria Sueli SilvaSilva,Sônia Mara NunesBasques,Fernando ValadaresRibas,João GabrielCarneiro-Proietti,Anna Bárbara de FreitasMartins,Marina Lobato HTLV Blood donors Real-time PCR Western blot INTRODUCTION: HTLV-1/2 screening among blood donors commonly utilizes an enzyme-linked immunosorbent assay (EIA), followed by a confirmatory method such as Western blot (WB) if the EIA is positive. However, this algorithm yields a high rate of inconclusive results, and is expensive. METHODS: Two qualitative real-time PCR assays were developed to detect HTLV-1 and 2, and a total of 318 samples were tested (152 blood donors, 108 asymptomatic carriers, 26 HAM/TSP patients and 30 seronegative individuals). RESULTS: The sensitivity and specificity of PCR in comparison with WB results were 99.4% and 98.5%, respectively. PCR tests were more efficient for identifying the virus type, detecting HTLV-2 infection and defining inconclusive cases. CONCLUSIONS: Because real-time PCR is sensitive and practical and costs much less than WB, this technique can be used as a confirmatory test for HTLV in blood banks, as a replacement for WB.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.43 n.2 20102010-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822010000200001en10.1590/S0037-86822010000200001 |
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Andrade,Rafaela Gomes Ribeiro,Maísa Aparecida Namen-Lopes,Maria Sueli Silva Silva,Sônia Mara Nunes Basques,Fernando Valadares Ribas,João Gabriel Carneiro-Proietti,Anna Bárbara de Freitas Martins,Marina Lobato |
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Andrade,Rafaela Gomes Ribeiro,Maísa Aparecida Namen-Lopes,Maria Sueli Silva Silva,Sônia Mara Nunes Basques,Fernando Valadares Ribas,João Gabriel Carneiro-Proietti,Anna Bárbara de Freitas Martins,Marina Lobato Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors |
author_facet |
Andrade,Rafaela Gomes Ribeiro,Maísa Aparecida Namen-Lopes,Maria Sueli Silva Silva,Sônia Mara Nunes Basques,Fernando Valadares Ribas,João Gabriel Carneiro-Proietti,Anna Bárbara de Freitas Martins,Marina Lobato |
author_sort |
Andrade,Rafaela Gomes |
title |
Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors |
title_short |
Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors |
title_full |
Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors |
title_fullStr |
Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors |
title_full_unstemmed |
Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors |
title_sort |
evaluation of the use of real-time pcr for human t cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors |
description |
INTRODUCTION: HTLV-1/2 screening among blood donors commonly utilizes an enzyme-linked immunosorbent assay (EIA), followed by a confirmatory method such as Western blot (WB) if the EIA is positive. However, this algorithm yields a high rate of inconclusive results, and is expensive. METHODS: Two qualitative real-time PCR assays were developed to detect HTLV-1 and 2, and a total of 318 samples were tested (152 blood donors, 108 asymptomatic carriers, 26 HAM/TSP patients and 30 seronegative individuals). RESULTS: The sensitivity and specificity of PCR in comparison with WB results were 99.4% and 98.5%, respectively. PCR tests were more efficient for identifying the virus type, detecting HTLV-2 infection and defining inconclusive cases. CONCLUSIONS: Because real-time PCR is sensitive and practical and costs much less than WB, this technique can be used as a confirmatory test for HTLV in blood banks, as a replacement for WB. |
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Sociedade Brasileira de Medicina Tropical - SBMT |
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2010 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822010000200001 |
work_keys_str_mv |
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