Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection

Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection

A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100% agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivity of this technique even more and confers specificity to the diagnosis. The technique described in this work will be useful in rabies diagnosis laboratories, once the cost is compatible with traditional rabies diagnostic techniques.

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Bibliographic Details
Main Authors: Carnieli Junior,Pedro, Ventura,Armando Moraes, Durigon,Edison Luiz
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2006
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822006000200005
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spelling oai:scielo:S0037-868220060002000052006-05-05Digoxigenin-labeled probe for rabies virus nucleoprotein gene detectionCarnieli Junior,PedroVentura,Armando MoraesDurigon,Edison Luiz Rabies N Gene Reverse transcription polymerase chain reaction Probe Hybridization A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100% agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivity of this technique even more and confers specificity to the diagnosis. The technique described in this work will be useful in rabies diagnosis laboratories, once the cost is compatible with traditional rabies diagnostic techniques.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.39 n.2 20062006-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822006000200005en10.1590/S0037-86822006000200005
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Carnieli Junior,Pedro
Ventura,Armando Moraes
Durigon,Edison Luiz
spellingShingle Carnieli Junior,Pedro
Ventura,Armando Moraes
Durigon,Edison Luiz
Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection
author_facet Carnieli Junior,Pedro
Ventura,Armando Moraes
Durigon,Edison Luiz
author_sort Carnieli Junior,Pedro
title Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection
title_short Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection
title_full Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection
title_fullStr Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection
title_full_unstemmed Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection
title_sort digoxigenin-labeled probe for rabies virus nucleoprotein gene detection
description A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100% agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivity of this technique even more and confers specificity to the diagnosis. The technique described in this work will be useful in rabies diagnosis laboratories, once the cost is compatible with traditional rabies diagnostic techniques.
publisher Sociedade Brasileira de Medicina Tropical - SBMT
publishDate 2006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822006000200005
work_keys_str_mv AT carnielijuniorpedro digoxigeninlabeledprobeforrabiesvirusnucleoproteingenedetection
AT venturaarmandomoraes digoxigeninlabeledprobeforrabiesvirusnucleoproteingenedetection
AT durigonedisonluiz digoxigeninlabeledprobeforrabiesvirusnucleoproteingenedetection
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