Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients
Abdominal angiostrongyliasis is a zoonotic infection caused by an intra-vascular nematode parasitic of wild rodents, Angiostrongylus costaricensis. No parasitological diagnosis is currently available and immunodiagnosis presents several drawbacks. Primers constructed based on a congeneric species, A. cantonensis, were able to amplify a 232 bp fragment from serum samples of 3 patients with histopathological diagnosis. Extraction was better performed with DNAzol and the specificity of the primers was confirmed by Southern blot. This disease has been diagnosed with frequency in south of Brazil, thus, this method appears like the important and unpublished alternative to improve diagnostic of disease.
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Instituto de Medicina Tropical de São Paulo
2003
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oai:scielo:S0036-466520030005000112003-11-11Diagnosis of abdominal angiostrongyliasis by PCR from sera of patientsSilva,Ana Cristina Arámburu daGraeff-Teixeira,CarlosZaha,Arnaldo Angiostrongylus costaricensis Eosinophilic gastroenteritis PCR Abdominal angiostrongyliasis is a zoonotic infection caused by an intra-vascular nematode parasitic of wild rodents, Angiostrongylus costaricensis. No parasitological diagnosis is currently available and immunodiagnosis presents several drawbacks. Primers constructed based on a congeneric species, A. cantonensis, were able to amplify a 232 bp fragment from serum samples of 3 patients with histopathological diagnosis. Extraction was better performed with DNAzol and the specificity of the primers was confirmed by Southern blot. This disease has been diagnosed with frequency in south of Brazil, thus, this method appears like the important and unpublished alternative to improve diagnostic of disease.info:eu-repo/semantics/openAccessInstituto de Medicina Tropical de São PauloRevista do Instituto de Medicina Tropical de São Paulo v.45 n.5 20032003-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652003000500011en10.1590/S0036-46652003000500011 |
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Silva,Ana Cristina Arámburu da Graeff-Teixeira,Carlos Zaha,Arnaldo |
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Silva,Ana Cristina Arámburu da Graeff-Teixeira,Carlos Zaha,Arnaldo Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients |
| author_facet |
Silva,Ana Cristina Arámburu da Graeff-Teixeira,Carlos Zaha,Arnaldo |
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Silva,Ana Cristina Arámburu da |
| title |
Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients |
| title_short |
Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients |
| title_full |
Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients |
| title_fullStr |
Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients |
| title_full_unstemmed |
Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients |
| title_sort |
diagnosis of abdominal angiostrongyliasis by pcr from sera of patients |
| description |
Abdominal angiostrongyliasis is a zoonotic infection caused by an intra-vascular nematode parasitic of wild rodents, Angiostrongylus costaricensis. No parasitological diagnosis is currently available and immunodiagnosis presents several drawbacks. Primers constructed based on a congeneric species, A. cantonensis, were able to amplify a 232 bp fragment from serum samples of 3 patients with histopathological diagnosis. Extraction was better performed with DNAzol and the specificity of the primers was confirmed by Southern blot. This disease has been diagnosed with frequency in south of Brazil, thus, this method appears like the important and unpublished alternative to improve diagnostic of disease. |
| publisher |
Instituto de Medicina Tropical de São Paulo |
| publishDate |
2003 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652003000500011 |
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