Differences between in vitro and in vivo obtained schistosomules

Differences between in vitro and in vivo obtained schistosomules

The injection of cercariae of Schistosoma mansoni into the peritoneal cavity of naive mice induces cell adhesion to these larvae, and this adherence sharply decreases when the infecting larva changes to schistosomule. This procedure was used to detect differences between schistosomules obtained in vivo and in vitro. Reinoculation of schistosomules obtained in vivo into the peritoneal cavity of mice did not trigger cell adhesion. In contrast, adherent cells were found in 4 and 24-hour-in vitro schistosomules. Our data on schistosomules obtained in vitro indicate that more than 24 hours are needed for complete remotion of molecules involved in the phenomenon of cell adhesion.

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Main Authors: Melo,Alan L., Machado,Conceição R. S., Pereira,Leógenes H.
Format: Digital revista
Language:English
Published: Instituto de Medicina Tropical de São Paulo 1990
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651990000200003
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spelling oai:scielo:S0036-466519900002000032006-09-13Differences between in vitro and in vivo obtained schistosomulesMelo,Alan L.Machado,Conceição R. S.Pereira,Leógenes H. Schistosoma mansoni transformation Schistosomula in vivo in vitro The injection of cercariae of Schistosoma mansoni into the peritoneal cavity of naive mice induces cell adhesion to these larvae, and this adherence sharply decreases when the infecting larva changes to schistosomule. This procedure was used to detect differences between schistosomules obtained in vivo and in vitro. Reinoculation of schistosomules obtained in vivo into the peritoneal cavity of mice did not trigger cell adhesion. In contrast, adherent cells were found in 4 and 24-hour-in vitro schistosomules. Our data on schistosomules obtained in vitro indicate that more than 24 hours are needed for complete remotion of molecules involved in the phenomenon of cell adhesion.info:eu-repo/semantics/openAccessInstituto de Medicina Tropical de São PauloRevista do Instituto de Medicina Tropical de São Paulo v.32 n.2 19901990-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651990000200003en10.1590/S0036-46651990000200003
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Melo,Alan L.
Machado,Conceição R. S.
Pereira,Leógenes H.
spellingShingle Melo,Alan L.
Machado,Conceição R. S.
Pereira,Leógenes H.
Differences between in vitro and in vivo obtained schistosomules
author_facet Melo,Alan L.
Machado,Conceição R. S.
Pereira,Leógenes H.
author_sort Melo,Alan L.
title Differences between in vitro and in vivo obtained schistosomules
title_short Differences between in vitro and in vivo obtained schistosomules
title_full Differences between in vitro and in vivo obtained schistosomules
title_fullStr Differences between in vitro and in vivo obtained schistosomules
title_full_unstemmed Differences between in vitro and in vivo obtained schistosomules
title_sort differences between in vitro and in vivo obtained schistosomules
description The injection of cercariae of Schistosoma mansoni into the peritoneal cavity of naive mice induces cell adhesion to these larvae, and this adherence sharply decreases when the infecting larva changes to schistosomule. This procedure was used to detect differences between schistosomules obtained in vivo and in vitro. Reinoculation of schistosomules obtained in vivo into the peritoneal cavity of mice did not trigger cell adhesion. In contrast, adherent cells were found in 4 and 24-hour-in vitro schistosomules. Our data on schistosomules obtained in vitro indicate that more than 24 hours are needed for complete remotion of molecules involved in the phenomenon of cell adhesion.
publisher Instituto de Medicina Tropical de São Paulo
publishDate 1990
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651990000200003
work_keys_str_mv AT meloalanl differencesbetweeninvitroandinvivoobtainedschistosomules
AT machadoconceicaors differencesbetweeninvitroandinvivoobtainedschistosomules
AT pereiraleogenesh differencesbetweeninvitroandinvivoobtainedschistosomules
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