Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae

Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae

Background: Integrons are responsible for the capture and dissemination of resistance genes in Gram-negative bacteria. Aim: To characterize the variable region within class 1 integrons in nosocomial strains of Klebsiella pneumoniae. Material and Methods: Twenty-nine Klebsiella pneumoniae strains isolated from hospitalized patients were analyzed. The variable region of class 1 integrons was characterized by polymerase chain reaction and direct sequencing. Genetic localization of class 1 integrons was determined by bacterial conjugation. Results: Ten strains contained class 1 integrons, with sizes ranging from 750 to 2000 base pairs. One integrant element was present in nine strains and two elements in one single strain. Integrons were associated to plasmids. Cassettes aadA, aac(6)-Iq, orfD, dfrA]7, aadA5 and aadB were found. Conclusions: The presence of class 1 integrons may play an important role in the dissemination of hospital resistance against amino glycosides.

Saved in:
Bibliographic Details
Main Authors: GUZMÁN,MILITZA, ALONSO,GUILLERMINA
Format: Digital revista
Language:Spanish / Castilian
Published: Sociedad Médica de Santiago 2010
Online Access:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872010000300010
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0034-98872010000300010
record_format ojs
spelling oai:scielo:S0034-988720100003000102010-05-19Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniaeGUZMÁN,MILITZAALONSO,GUILLERMINA Bacterial infections Integrons Klebsiella pneumoniae Background: Integrons are responsible for the capture and dissemination of resistance genes in Gram-negative bacteria. Aim: To characterize the variable region within class 1 integrons in nosocomial strains of Klebsiella pneumoniae. Material and Methods: Twenty-nine Klebsiella pneumoniae strains isolated from hospitalized patients were analyzed. The variable region of class 1 integrons was characterized by polymerase chain reaction and direct sequencing. Genetic localization of class 1 integrons was determined by bacterial conjugation. Results: Ten strains contained class 1 integrons, with sizes ranging from 750 to 2000 base pairs. One integrant element was present in nine strains and two elements in one single strain. Integrons were associated to plasmids. Cassettes aadA, aac(6)-Iq, orfD, dfrA]7, aadA5 and aadB were found. Conclusions: The presence of class 1 integrons may play an important role in the dissemination of hospital resistance against amino glycosides.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.138 n.3 20102010-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872010000300010es10.4067/S0034-98872010000300010
institution SCIELO
collection OJS
country Chile
countrycode CL
component Revista
access En linea
databasecode rev-scielo-cl
tag revista
region America del Sur
libraryname SciELO
language Spanish / Castilian
format Digital
author GUZMÁN,MILITZA
ALONSO,GUILLERMINA
spellingShingle GUZMÁN,MILITZA
ALONSO,GUILLERMINA
Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae
author_facet GUZMÁN,MILITZA
ALONSO,GUILLERMINA
author_sort GUZMÁN,MILITZA
title Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae
title_short Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae
title_full Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae
title_fullStr Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae
title_full_unstemmed Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae
title_sort caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de klebsiella pneumoniae
description Background: Integrons are responsible for the capture and dissemination of resistance genes in Gram-negative bacteria. Aim: To characterize the variable region within class 1 integrons in nosocomial strains of Klebsiella pneumoniae. Material and Methods: Twenty-nine Klebsiella pneumoniae strains isolated from hospitalized patients were analyzed. The variable region of class 1 integrons was characterized by polymerase chain reaction and direct sequencing. Genetic localization of class 1 integrons was determined by bacterial conjugation. Results: Ten strains contained class 1 integrons, with sizes ranging from 750 to 2000 base pairs. One integrant element was present in nine strains and two elements in one single strain. Integrons were associated to plasmids. Cassettes aadA, aac(6)-Iq, orfD, dfrA]7, aadA5 and aadB were found. Conclusions: The presence of class 1 integrons may play an important role in the dissemination of hospital resistance against amino glycosides.
publisher Sociedad Médica de Santiago
publishDate 2010
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872010000300010
work_keys_str_mv AT guzmanmilitza caracterizaciondelaregionvariabledeintegronesclase1presentesencepasnosocomialesdeklebsiellapneumoniae
AT alonsoguillermina caracterizaciondelaregionvariabledeintegronesclase1presentesencepasnosocomialesdeklebsiellapneumoniae
_version_ 1755988316021325824

Similar Items