Mutación del gen K-ras en el cáncer de la vesícula biliar

Background: Different K-ras mutation frequencies in gallbladder cancer have been reported. Aim: To study the frequency of K-ras gene mutations in advanced gallbladder carcinoma not associated to anomalous junction of pancreatic-biliary duct (AJPBD). Material and methods: 33 formalin fixed paraffin embedded samples of gallbladder carcinoma (30 women, age range 32-86 years) were selected. Pancreatic cancer tissue with K-ras mutations was used as control. DNA was extracted from the histological section by mean of microdissection and K-ras mutations in codon 12 were detected by polymerase chain reaction and restriction fragment length polymorphism (RFLP), using previously reported technique. Results: Most cases were poorly differentiated adenocarcinomas. K-ras mutation was detected in 10 cases (30%) samples. No differences in K-ras mutation frequency between subserous and serous tumors were detected and no relation between histological features and the mutation was observed. Conclusions: K-ras mutation in codon 12 is present in 30% in our advanced gallbladder carcinomas. The study of K-ras mutation in preneoplastic lesions and early carcinomas will be important to determine the role of this gene in the gallbladder carcinogenesis in Chile (Rev Méd Chile 2004; 132: 955-60)

Bibliographic Details

Main Authors:	Roa S,Juan Carlos, Roa E,Iván, Aretxabala U,Xabier de, Melo A,Angélica, Faría O,Gaspar, Tapia E,Oscar
Format:	Digital revista
Language:	Spanish / Castilian
Published:	Sociedad Médica de Santiago 2004
Online Access:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872004000800007
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