Dexmedetomidine preconditioning protects against lipopolysaccharides-induced injury in the human alveolar epithelial cells
Abstract Background and objectives Dexmedetomidine (DEX) has demonstrated the preconditioning effect and shown protective effects against organize injury. In this study, using A549 (human alveolar epithelial cell) cell lines, we investigated whether DEX preconditioning protected against acute lung injury (ALI) in vitro. Methods A549 were randomly divided into four groups (n = 5): control group, DEX group, lipopolysaccharides (LPS) group, and D-LPS (DEX + LPS) group. Phosphate buffer saline (PBS) or DEX were administered. After 2 h preconditioning, the medium was refreshed and the cells were challenged with LPS for 24 h on the LPS and D-LPS group. Then the malondialdehyde (MDA), superoxide dismutase (SOD), Bcl-2, Bax, caspase-3 and the cytochrome c in the A549 were tested. The apoptosis was also evaluated in the cells. Results Compare with LPS group, DEX preconditioning reduced the apoptosis (26.43% ± 1.05% vs. 33.58% ± 1.16%, p < 0.05) in the A549, which is correlated with decreased MDA (12.84 ± 1.05 vs. 19.16 ± 1.89 nmoL.mg-1 protein, p < 0.05) and increased SOD activity (30.28 ± 2.38 vs. 20.86 ± 2.19 U.mg-1 protein, p < 0.05). DEX preconditioning also increased the Bcl-2 level (0.53 ± 0.03 vs. 0.32 ± 0.04, p < 0.05) and decreased the level of Bax (0.49 ± 0.04 vs. 0.65 ± 0.04, p < 0.05), caspase-3 (0.54 ± 0.04 vs. 0.76 ± 0.04, p < 0.05) and cytochrome c. Conclusion DEX preconditioning has a protective effect against ALI in vitro. The potential mechanisms involved are the inhibition of cell death and improvement of antioxidation.
Main Authors: | , , , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Anestesiologia
2017
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-70942017000600600 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0034-70942017000600600 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0034-709420170006006002017-11-29Dexmedetomidine preconditioning protects against lipopolysaccharides-induced injury in the human alveolar epithelial cellsZhang,LeiZhou,Xian-JinZhan,Li-YingWu,Xiao-JingLi,Wen-LanZhao,BoMeng,Qing-TaoXia,Zhong-Yuan Dexmedetomidine Lipopolysaccharides Preconditioning Acute lung injury Alveolar epithelial cell Abstract Background and objectives Dexmedetomidine (DEX) has demonstrated the preconditioning effect and shown protective effects against organize injury. In this study, using A549 (human alveolar epithelial cell) cell lines, we investigated whether DEX preconditioning protected against acute lung injury (ALI) in vitro. Methods A549 were randomly divided into four groups (n = 5): control group, DEX group, lipopolysaccharides (LPS) group, and D-LPS (DEX + LPS) group. Phosphate buffer saline (PBS) or DEX were administered. After 2 h preconditioning, the medium was refreshed and the cells were challenged with LPS for 24 h on the LPS and D-LPS group. Then the malondialdehyde (MDA), superoxide dismutase (SOD), Bcl-2, Bax, caspase-3 and the cytochrome c in the A549 were tested. The apoptosis was also evaluated in the cells. Results Compare with LPS group, DEX preconditioning reduced the apoptosis (26.43% ± 1.05% vs. 33.58% ± 1.16%, p < 0.05) in the A549, which is correlated with decreased MDA (12.84 ± 1.05 vs. 19.16 ± 1.89 nmoL.mg-1 protein, p < 0.05) and increased SOD activity (30.28 ± 2.38 vs. 20.86 ± 2.19 U.mg-1 protein, p < 0.05). DEX preconditioning also increased the Bcl-2 level (0.53 ± 0.03 vs. 0.32 ± 0.04, p < 0.05) and decreased the level of Bax (0.49 ± 0.04 vs. 0.65 ± 0.04, p < 0.05), caspase-3 (0.54 ± 0.04 vs. 0.76 ± 0.04, p < 0.05) and cytochrome c. Conclusion DEX preconditioning has a protective effect against ALI in vitro. The potential mechanisms involved are the inhibition of cell death and improvement of antioxidation.info:eu-repo/semantics/openAccessSociedade Brasileira de AnestesiologiaRevista Brasileira de Anestesiologia v.67 n.6 20172017-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-70942017000600600en10.1016/j.bjane.2017.02.002 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Zhang,Lei Zhou,Xian-Jin Zhan,Li-Ying Wu,Xiao-Jing Li,Wen-Lan Zhao,Bo Meng,Qing-Tao Xia,Zhong-Yuan |
spellingShingle |
Zhang,Lei Zhou,Xian-Jin Zhan,Li-Ying Wu,Xiao-Jing Li,Wen-Lan Zhao,Bo Meng,Qing-Tao Xia,Zhong-Yuan Dexmedetomidine preconditioning protects against lipopolysaccharides-induced injury in the human alveolar epithelial cells |
author_facet |
Zhang,Lei Zhou,Xian-Jin Zhan,Li-Ying Wu,Xiao-Jing Li,Wen-Lan Zhao,Bo Meng,Qing-Tao Xia,Zhong-Yuan |
author_sort |
Zhang,Lei |
title |
Dexmedetomidine preconditioning protects against lipopolysaccharides-induced injury in the human alveolar epithelial cells |
title_short |
Dexmedetomidine preconditioning protects against lipopolysaccharides-induced injury in the human alveolar epithelial cells |
title_full |
Dexmedetomidine preconditioning protects against lipopolysaccharides-induced injury in the human alveolar epithelial cells |
title_fullStr |
Dexmedetomidine preconditioning protects against lipopolysaccharides-induced injury in the human alveolar epithelial cells |
title_full_unstemmed |
Dexmedetomidine preconditioning protects against lipopolysaccharides-induced injury in the human alveolar epithelial cells |
title_sort |
dexmedetomidine preconditioning protects against lipopolysaccharides-induced injury in the human alveolar epithelial cells |
description |
Abstract Background and objectives Dexmedetomidine (DEX) has demonstrated the preconditioning effect and shown protective effects against organize injury. In this study, using A549 (human alveolar epithelial cell) cell lines, we investigated whether DEX preconditioning protected against acute lung injury (ALI) in vitro. Methods A549 were randomly divided into four groups (n = 5): control group, DEX group, lipopolysaccharides (LPS) group, and D-LPS (DEX + LPS) group. Phosphate buffer saline (PBS) or DEX were administered. After 2 h preconditioning, the medium was refreshed and the cells were challenged with LPS for 24 h on the LPS and D-LPS group. Then the malondialdehyde (MDA), superoxide dismutase (SOD), Bcl-2, Bax, caspase-3 and the cytochrome c in the A549 were tested. The apoptosis was also evaluated in the cells. Results Compare with LPS group, DEX preconditioning reduced the apoptosis (26.43% ± 1.05% vs. 33.58% ± 1.16%, p < 0.05) in the A549, which is correlated with decreased MDA (12.84 ± 1.05 vs. 19.16 ± 1.89 nmoL.mg-1 protein, p < 0.05) and increased SOD activity (30.28 ± 2.38 vs. 20.86 ± 2.19 U.mg-1 protein, p < 0.05). DEX preconditioning also increased the Bcl-2 level (0.53 ± 0.03 vs. 0.32 ± 0.04, p < 0.05) and decreased the level of Bax (0.49 ± 0.04 vs. 0.65 ± 0.04, p < 0.05), caspase-3 (0.54 ± 0.04 vs. 0.76 ± 0.04, p < 0.05) and cytochrome c. Conclusion DEX preconditioning has a protective effect against ALI in vitro. The potential mechanisms involved are the inhibition of cell death and improvement of antioxidation. |
publisher |
Sociedade Brasileira de Anestesiologia |
publishDate |
2017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-70942017000600600 |
work_keys_str_mv |
AT zhanglei dexmedetomidinepreconditioningprotectsagainstlipopolysaccharidesinducedinjuryinthehumanalveolarepithelialcells AT zhouxianjin dexmedetomidinepreconditioningprotectsagainstlipopolysaccharidesinducedinjuryinthehumanalveolarepithelialcells AT zhanliying dexmedetomidinepreconditioningprotectsagainstlipopolysaccharidesinducedinjuryinthehumanalveolarepithelialcells AT wuxiaojing dexmedetomidinepreconditioningprotectsagainstlipopolysaccharidesinducedinjuryinthehumanalveolarepithelialcells AT liwenlan dexmedetomidinepreconditioningprotectsagainstlipopolysaccharidesinducedinjuryinthehumanalveolarepithelialcells AT zhaobo dexmedetomidinepreconditioningprotectsagainstlipopolysaccharidesinducedinjuryinthehumanalveolarepithelialcells AT mengqingtao dexmedetomidinepreconditioningprotectsagainstlipopolysaccharidesinducedinjuryinthehumanalveolarepithelialcells AT xiazhongyuan dexmedetomidinepreconditioningprotectsagainstlipopolysaccharidesinducedinjuryinthehumanalveolarepithelialcells |
_version_ |
1756376353289011200 |